A novel aerobic, Gram-negative bacterial strain, designated 5IX/A01/131, was isolated from waters in the coastal north-western Mediterranean Sea. The cells were motile, straight rods, 1.6 μm long and 0.5 μm wide, and formed cream colonies on marine 2216 agar. The G+C content of the genomic DNA was 57 mol %. Phylogenetic analysis of the 16S rRNA gene sequence placed the strain in the class . On the basis of the 16S rRNA gene sequence comparisons and physiological and biochemical characteristics, strain 5IX/A01/131 represents a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain of is 5IX/A01/131 (=DSM 19753 =CIP 109757 =MOLA 225).


Article metrics loading...

Loading full text...

Full text loading...



  1. Agogué, H., Casamayor, E. O., Joux, F., Obernosterer, I., Dupuy, C., Lantoine, F., Catala, P., Weinbauer, M. G., Reinthaler, T. & other authors(2004). Comparison of samplers for the biological characterization of the sea surface microlayer. Limnol Oceanogr Methods 2, 213–225.[CrossRef] [Google Scholar]
  2. Agogué, H., Casamayor, E. O., Bourrain, M., Obernosterer, I., Joux, F., Herndl, G. J. & Lebaron, P.(2005). A survey on bacteria inhabiting the sea surface microlayer of coastal ecosystems. FEMS Microbiol Ecol 54, 269–280.[CrossRef] [Google Scholar]
  3. Gonzalez, J. M., Mayer, F., Moran, M. A., Hodson, R. E. & Whitman, W. B.(1997).Microbulbifer hydrolyticus gen. nov., sp. nov., and Marinobacterium georgiense gen. nov., sp. nov., two marine bacteria from a lignin-rich pulp mill waste enrichment community. Int J Syst Bacteriol 47, 369–376.[CrossRef] [Google Scholar]
  4. Humm, H. J.(1946). Marine agar-digesting bacteria of the South Atlantic coast. Duke Univ Mar Stn Bull 3, 45–75. [Google Scholar]
  5. Marmur, J. & Doty, P.(1962). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef] [Google Scholar]
  6. Palleroni, N. J.(1984). Genus Pseudomonas Migula 1894. In Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 141–199. Edited by N. R. Krieg & J. G. Holt. Baltimore: Williams & Wilkins.
  7. Powers, E. M.(1995). Efficacy of the Ryu nonstaining KOH technique for rapidly determining Gram reactions of food-borne and waterborne bacteria and yeasts. Appl Environ Microbiol 61, 3756–3758. [Google Scholar]
  8. Raguénès, G., Christen, R., Guézennec, J., Pignet, P. & Barbier, G.(1997).Vibrio diabolicus sp. nov., a new polysaccharide-secreting organism isolated from a deep-sea hydrothermal vent polychete annelid, Alvinella pompejana. Int J Syst Bacteriol 47, 989–995.[CrossRef] [Google Scholar]
  9. Tang, Y. W., Ellis, N. M., Hopkins, M. K., Smith, D. H., Dodge, D. E. & Persing, D. H.(1998). Comparison of phenotypic and genotypic techniques for identification of unusual aerobic pathogenic gram-negative bacilli. J Clin Microbiol 36, 3674–3679. [Google Scholar]
  10. Urios, L., Agogué, H., Lesongeur, F., Stackebrandt, E. & Lebaron, P.(2006).Balneola vulgaris gen. nov., sp. nov., a member of the phylum Bacteroidetes from the north-western Mediterranean Sea. Int J Syst Evol Microbiol 56, 1883–1887.[CrossRef] [Google Scholar]
  11. Urios, L., Intertaglia, L., Lesongeur, F. & Lebaron, P.(2008).Haliea salexigens gen. nov., sp. nov., a member of the Gammaproteobacteria from the Mediterranean Sea. Int J Syst Evol Microbiol 58, 1233–1237.[CrossRef] [Google Scholar]
  12. Wery, N., Lesongeur, F., Pignet, P., Derennes, V., Cambon-Bonavita, M.-A., Godfroy, A. & Barbier, G.(2001a).Marinitoga camini gen. nov., sp. nov., a rod-shaped bacterium belonging to the order Thermotogales, isolated from a deep-sea hydrothermal vent. Int J Syst Evol Microbiol 51, 495–504. [Google Scholar]
  13. Wery, N., Moricet, J., Cueff, V., Jean, J., Pignet, P., Lesongeur, F., Cambon-Bonavita, M.-A. & Barbier, G.(2001b).Caloranaerobacter azorensis gen. nov., sp. nov., an anaerobic thermophilic bacterium isolated from a deep-sea hydrothermal vent. Int J Syst Evol Microbiol 51, 1789–1796.[CrossRef] [Google Scholar]
  14. Yoon, J. H., Kim, I. G., Shin, D. Y., Kang, K. H. & Park, Y. H.(2003a).Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. Int J Syst Evol Microbiol 53, 53–57.[CrossRef] [Google Scholar]
  15. Yoon, J. H., Kim, I. G., Kang, K. H., Oh, T. K. & Park, Y. H.(2003b). Transfer of Pseudomonas elongata Humm 1946 to the genus Microbulbifer as Microbulbifer elongatus comb. nov. Int J Syst Evol Microbiol 53, 1357–1361.[CrossRef] [Google Scholar]
  16. Yoon, J. H., Kim, I. G., Oh, T. K. & Park, Y. H.(2004).Microbulbifer maritimus sp. nov., isolated from an intertidal sediment from the Yellow Sea, Korea. Int J Syst Evol Microbiol 54, 1111–1116.[CrossRef] [Google Scholar]
  17. Yoon, J. H., Jung, S. Y., Kang, S. J. & Oh, T. K.(2007).Microbulbifer celer sp. nov., isolated from a marine solar saltern of the Yellow Sea in Korea. Int J Syst Evol Microbiol 57, 2365–2369.[CrossRef] [Google Scholar]

Data & Media loading...


Electron micrograph of a negatively stained cell of strain 5IX/A01/131 harvested during the exponential phase. A single polar flagellum is visible. Bar, 0.5 µm.


Effects of pH, salinity and temperature on growth of strain 5IX/A01/131 . [PDF](23 KB)


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error