The taxonomic position of a lactic acid bacterium, strain L13, isolated from senmaizuke, a Japanese traditional pickle, was studied. This strain was a heterofermentative, facultatively anaerobic, Gram-positive, non-spore-forming, non-motile, rod-shaped bacterium. It produced -lactic acid from glucose. Phylogenetic analysis of its 16S rRNA gene sequence and physiological and biochemical characteristics indicated that the strain was a member of the genus . Based on 16S rRNA gene sequence similarity, strain L13 was closely related to CIP 108387 (98.9 %) and ATCC 53295 (98.5 %). In addition, the gene sequence of strain L13 was closely related to those of CIP 108387 (92.2 %) and ATCC 53295 (92.0 %). However, DNA–DNA hybridization of strain L13 with these two strains revealed that strain L13 represents a separate genomic species. The DNA GC content of strain L13 was 46 mol% and the peptidoglycan was of the A4 -Lys–-Asp type. Thus, these data indicate that strain L13 represents a novel species of the genus , for which the name is proposed. The type strain is L13 (=NBRC 103853=TISTR 1847).


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vol. , part 7, pp. 1625 - 1629

Phylogenetic trees based on 16S rRNA gene sequences derived by the neighbour-joining, maximum-likelihood and maximum-parsimony methods, respectively, showing the position of L13 among selected lactobacilli. [ PDF] 108 KB

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