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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 58, Issue 10

sp. nov., isolated from a Thai medicinal plant Free

Abstract

A novel, Gram-positive, motile, coccus-shaped, orange-pigmented organism, designated strain KKD096, was isolated from the roots of a Thai medicinal plant, DC. var. Thwaites. Growth of strain KKD096 occurred at temperatures of 14–34 °C, at pH 5.0–9.0 and at NaCl concentrations up to 7 % (w/v). Whole-cell hydrolysates contained arabinose and galactose as the characteristic sugars. The diagnostic diamino acid of the peptidoglycan was -diaminopimelic acid. The glycan moiety of the murein contained acetyl residues. The predominant menaquinone was MK-9(H); mycolic acids were not detected. The genomic DNA G+C content was 73.3 mol%. The major cellular fatty acid was anteiso-C (81.42 % of the total). Strain KKD096 was assigned to the genus on the basis of 16S rRNA gene sequence analysis; it was most closely related to DSM 14245 (97.1 % similarity). DNA–DNA hybridization revealed 39.4 % relatedness between these two taxa. On the basis of the genotypic and phenotypic data presented, strain KKD096 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is KKD096 (=TISTR 1856=NRRL B-24568=BCC 26245=NBRC 103943).

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Keyword(s): TEM, transmission electron microscopy
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2008-10-01
2025-05-20
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References

  1. Becker, B., Lechevalier, M. P. & Lechevalier, H. A.(1965). Chemical composition of cell-wall preparations from strains of various form-genera of aerobic actinomycetes. Appl Microbiol 13, 236–243. [Google Scholar]
  2. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E.(1977). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef] [Google Scholar]
  3. Duangmal, K., Ward, A. C. & Goodfellow, M.(2005). Selective isolation of members of the Streptomyces violaceoruber clade from soil. FEMS Microbiol Lett 245, 321–327.[CrossRef] [Google Scholar]
  4. Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in micro-dilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef] [Google Scholar]
  5. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  6. Gordon, R. E., Barnett, D. A., Handerhan, J. E. & Pang, C. H.-N.(1974).Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24, 54–63.[CrossRef] [Google Scholar]
  7. Hasegawa, T., Takizawa, M. & Tanida, S.(1983). A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29, 319–322.[CrossRef] [Google Scholar]
  8. Jukes, T. H. & Cantor, C. R.(1969). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  9. Küster, E. & Williams, S. T.(1964). Media for the isolation of streptomycetes: starch casein medium. Nature 202, 928–929.[CrossRef] [Google Scholar]
  10. Lee, S. D.(2006).Kineococcus marinus sp. nov., isolated from marine sediment of the coast of Jeju, Korea. Int J Syst Evol Microbiol 56, 1279–1283.[CrossRef] [Google Scholar]
  11. Marmur, J.(1961). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef] [Google Scholar]
  12. Phillips, R. W., Wiegel, J., Berry, C. J., Filermans, C., Peacock, A. D., White, D. C. & Shimkets, L. J.(2002).Kineococcus radiotolerans sp. nov., a radiation-resistant, Gram-positive bacterium. Int J Syst Evol Microbiol 52, 933–938.[CrossRef] [Google Scholar]
  13. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  14. Shirling, E. B. & Gottlieb, D.(1966). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef] [Google Scholar]
  15. Tamaoka, J. & Komagata, K.(1984). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef] [Google Scholar]
  16. Tamaoka, J., Katayara-Fujimura, Y. & Kuraishi, H.(1983). Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. J Appl Bacteriol 54, 31–36.[CrossRef] [Google Scholar]
  17. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  18. Tomiyasu, I.(1982). Mycolic acid composition and thermally adaptative changes in Nocardia asteroides. J Bacteriol 151, 828–837. [Google Scholar]
  19. Uchida, K. & Aida, K.(1984). An improved method for the glycolate test for simple identification of the acyl type of bacterial cell walls. J Gen Appl Microbiol 30, 131–134.[CrossRef] [Google Scholar]
  20. Van de Peer, Y. & De Wachter, R.(1994).treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10, 569–570. [Google Scholar]
  21. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on the reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  22. Yokota, A., Tamura, T., Nishii, T. & Hasegawa, T.(1993).Kineococcus aurantiacus gen. nov., sp. nov., a new aerobic, gram-positive, motile coccus with meso-diaminopimelic acid and arabinogalactan in the cell wall. Int J Syst Bacteriol 43, 52–57.[CrossRef] [Google Scholar]
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Kineococcus gynurae sp. nov., isolated from a Thai medicinal plant
Int J Syst Evol Microbiol 58, 2439 (2008); https://doi.org/10.1099/ijs.0.65671-0
/content/journal/ijsem/10.1099/ijs.0.65671-0
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