1887

Abstract

A novel, Gram-positive, motile, coccus-shaped, orange-pigmented organism, designated strain KKD096, was isolated from the roots of a Thai medicinal plant, DC. var. Thwaites. Growth of strain KKD096 occurred at temperatures of 14–34 °C, at pH 5.0–9.0 and at NaCl concentrations up to 7 % (w/v). Whole-cell hydrolysates contained arabinose and galactose as the characteristic sugars. The diagnostic diamino acid of the peptidoglycan was -diaminopimelic acid. The glycan moiety of the murein contained acetyl residues. The predominant menaquinone was MK-9(H); mycolic acids were not detected. The genomic DNA G+C content was 73.3 mol%. The major cellular fatty acid was anteiso-C (81.42 % of the total). Strain KKD096 was assigned to the genus on the basis of 16S rRNA gene sequence analysis; it was most closely related to DSM 14245 (97.1 % similarity). DNA–DNA hybridization revealed 39.4 % relatedness between these two taxa. On the basis of the genotypic and phenotypic data presented, strain KKD096 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is KKD096 (=TISTR 1856=NRRL B-24568=BCC 26245=NBRC 103943).

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2008-10-01
2022-01-27
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