1887

Abstract

A Gram-negative, pleomorphic, rod-shaped, non-spore-forming bacterium, designated strain NS05, was isolated from soil after enrichment with oxalate. On the basis of 16S rRNA gene sequence similarity, strain NS05 was shown to be phylogenetically related to the genera , and (96.3–98.1 % sequence similarity), class . Strain NS05 was most closely related to AS 1.1761 (98.1 % sequence similarity). The whole-cell fatty acid pattern of strain NS05 was typical of those found in members of the genus . Its main components were C 7 (60.4 %), C 8 cyclo (28.3 %) and C (7.4 %) and hydroxylated compounds were absent. The results of DNA–DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain NS05 with respect to the four species with validly published names. Therefore, it is concluded that NS05 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NS05 (=DSM 18746 =CCM 7435). Two other oxalate-utilizing strains, NS03 and NS04, isolated from paper-mill effluents, were shown by 16S rRNA gene sequence analysis to be affiliated to the species . The study of their physiological properties extends the knowledge of the physiological variability within this species.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65666-0
2008-09-01
2020-10-01
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/9/1997.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65666-0&mimeType=html&fmt=ahah

References

  1. Aragno, M. & Schlegel, H. G. ( 1992; ). The mesophilic hydrogen-oxidizing (Knallgas) bacteria. In The Prokaryotes, 2nd edn, pp. 344–384. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. Berlin: Springer.
  2. Cashion, P., Holder-Franklin, M. A., McCully, J. & Franklin, M. ( 1977; ). A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81, 461–466.[CrossRef]
    [Google Scholar]
  3. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E. ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef]
    [Google Scholar]
  4. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  5. De Soete, G. ( 1983; ). A least squares algorithm for fitting additive trees to proximity data. Psychometrika 48, 621–626.[CrossRef]
    [Google Scholar]
  6. Doronina, N. V., Darmaeva, Ts. D. & Trotsenko, Yu. A. ( 2001; ). Novel aerobic methylotrophic isolates from the soda lakes of the southern Transbaikal region. Microbiology (English translation of Mikrobiologiia) 70, 342–348.[CrossRef]
    [Google Scholar]
  7. Fritz, I., Strömpl, C. & Abraham, W.-R. ( 2004; ). Phylogenetic relationships of the genera Stella, Labrys and Angulomicrobium within the ‘Alphaproteobacteria’ and description of Angulomicrobium amanitiforme sp. nov. Int J Syst Evol Microbiol 54, 651–657.[CrossRef]
    [Google Scholar]
  8. Garrity, G. M., Bell, J. A. & Lilburn, T. ( 2005; ). Family VIII. Hyphomicrobiaceae Babudieri 1950, 589. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, part C, p. 476. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  9. Groth, I., Schumann, P., Rainey, F. A., Martin, K., Schuetze, B. & Augsten, K. ( 1997; ). Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol 47, 1129–1133.[CrossRef]
    [Google Scholar]
  10. Huß, V. A. R., Festl, H. & Schleifer, K. H. ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef]
    [Google Scholar]
  11. Kämpfer, P. & Kroppenstedt, R. M. ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef]
    [Google Scholar]
  12. Lee, K.-B., Liu, C.-T., Anzai, Y., Kim, H., Aono, T. & Oyaizu, H. ( 2005; ). The hierarchical system of the ‘Alphaproteobacteria’: description of Hyphomonadaceae fam. nov., Xanthobacteraceae fam. nov. and Erythrobacteraceae fam. nov. Int J Syst Evol Microbiol 55, 1907–1919.[CrossRef]
    [Google Scholar]
  13. Malik, K. A. ( 1988; ). A new freeze-drying method for the preservation of nitrogen-fixing and other fragile bacteria. J Microbiol Methods 8, 259–271.[CrossRef]
    [Google Scholar]
  14. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  15. Ørskov, J. ( 1928; ). Beschreibung eines neuen Mikroben, Microcyclus aquaticus, mit eigentuemlicher Morphologie. Zentralbl Bakteriol Parasitenkd Infektionskr Hyg Abt I 107, 180–184 (in German).
    [Google Scholar]
  16. Raj, H. D. ( 1983; ). Proposal of Ancylobacter gen. nov. as a substitute for the bacterial genus Microcyclus Ørskov 1928. Int J Syst Bacteriol 33, 397–398.[CrossRef]
    [Google Scholar]
  17. Raj, H. D. ( 1989; ). Oligotrophic methylotrophs: Ancylobacter (basonym “Microcyclus” Ørskov) Raj gen. nov. Crit Rev Microbiol 17, 89–106.[CrossRef]
    [Google Scholar]
  18. Sahin, N. ( 2003; ). Oxalotrophic bacteria. Res Microbiol 154, 399–407.[CrossRef]
    [Google Scholar]
  19. Sahin, N., Gökler, I. & Tamer, A. Ü. ( 2002; ). Isolation, characterization and numerical taxonomy of novel oxalate-oxidizing bacteria. J Microbiol 40, 109–118.
    [Google Scholar]
  20. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  21. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20, 16
    [Google Scholar]
  22. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–655. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  23. Somvanshi, V. S., Lang, E., Sträubler, B., Spröer, C., Schumann, P., Ganguly, S., Saxena, A. K. & Stackebrandt, E. ( 2006; ). Providencia vermicola sp. nov., isolated from infective juveniles of the entomopathogenic nematode Steinernema thermophilum. Int J Syst Evol Microbiol 56, 629–633.[CrossRef]
    [Google Scholar]
  24. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  25. Urakami, T. & Komagata, K. ( 1973; ). Cellular fatty acid composition and coenzyme Q system in Gram-negative methanol-utilizing bacteria. J Gen Appl Microbiol 25, 343–360.
    [Google Scholar]
  26. Urakami, T. & Komagata, K. ( 1986; ). Methanol-utilizing Ancylobacter strains and comparison of their cellular fatty acid compositions and quinone systems with those of Spirosoma, Flectobacillus, and Runella species. Int J Syst Bacteriol 36, 415–421.[CrossRef]
    [Google Scholar]
  27. Xin, Y. H., Zhou, Y. G., Zhou, H. L. & Chen, W. X. ( 2004; ). Ancylobacter rudongensis sp. nov., isolated from roots of Spartina anglica. Int J Syst Evol Microbiol 54, 385–388.[CrossRef]
    [Google Scholar]
  28. Xin, Y. H., Zhou, Y. G. & Chen, W. X. ( 2006; ). Ancylobacter polymorphus sp. nov. and Ancylobacter vacuolatus sp. nov. Int J Syst Evol Microbiol 56, 1185–1188.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65666-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65666-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error