A Gram-negative, pleomorphic, rod-shaped, non-spore-forming bacterium, designated strain NS05, was isolated from soil after enrichment with oxalate. On the basis of 16S rRNA gene sequence similarity, strain NS05 was shown to be phylogenetically related to the genera , and (96.3–98.1 % sequence similarity), class . Strain NS05 was most closely related to AS 1.1761 (98.1 % sequence similarity). The whole-cell fatty acid pattern of strain NS05 was typical of those found in members of the genus . Its main components were C 7 (60.4 %), C 8 cyclo (28.3 %) and C (7.4 %) and hydroxylated compounds were absent. The results of DNA–DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain NS05 with respect to the four species with validly published names. Therefore, it is concluded that NS05 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NS05 (=DSM 18746 =CCM 7435). Two other oxalate-utilizing strains, NS03 and NS04, isolated from paper-mill effluents, were shown by 16S rRNA gene sequence analysis to be affiliated to the species . The study of their physiological properties extends the knowledge of the physiological variability within this species.


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