A Gram-positive, endospore-forming, rod-shaped bacterium, designated strain B519, was isolated from a desert sand sample of Gansu Province, China. Strain B519 was strictly aerobic and cells were motile by means of peritrichous flagella. The strain grew optimally at 32–35 °C and pH 6.5–7.0. Chemotaxonomic data supported the affiliation of the new isolate to the genus , including menaquinone-7 (MK-7) as the major isoprenoid quinone, DNA G+C content of 49.9 mol%, cell-wall type A1 (-diaminopimelic acid as the diagnostic diamino acid) and anteiso-C, iso-C, C and iso-C as the major fatty acids. Comparative 16S rRNA gene sequence analysis showed that strain B519 was most closely related to KSL-134 (98.0 % similarity). DNA–DNA relatedness between strain B519 and KSL-134 was about 12.3 %. On the basis of phenotypic characteristics and molecular properties, strain B519 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is B519 (=KCTC 3951 =DSM 16969).


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Transmission electron micrograph showing general morphology of a negatively stained cell of strain B519 after growth for 2 days at 32 °C on R2A. Bar, 1 µm.


Extended neighbour-joining and maximum-parsimony trees showing phylogenetic relationships of strain B519 and related taxa. [PDF](22 KB)

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