sp. nov., isolated from a tidal flat of the Yellow Sea in Korea Free

Abstract

A Gram-negative, motile, ovoid to rod-shaped bacterial strain, designated strain SMK-122, was isolated from a Yellow Sea tidal flat located on the coast of Korea. Strain SMK-122 grew optimally at pH 7.0–8.0 and 30 °C. It contained Q-10 as the predominant ubiquinone and possessed C 7 and C as the major fatty acids. The DNA G+C content was 58.6 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain SMK-122 fell within the genus , being closest to ISM; the sequence similarities with respect to species ranged from 94.9 to 97.3 %. The mean value for DNA–DNA relatedness between strain SMK-122 and DSM 15170 was 13 %. Differential phenotypic properties of SMK-122, together with its phylogenetic and genetic distinctiveness, revealed that this strain is distinct from recognized species. On this basis, strain SMK-122 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SMK-122 (=KCTC 22174 =CCUG 55325).

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2008-05-01
2024-03-29
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References

  1. Baumann, P. & Baumann, L.(1981). The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes. In The Prokaryotes, pp. 1302–1331. Edited by M. P. Starr, H. Stolp, H. G. Trüper, A. Balows & H. G. Schlegel. Berlin: Springer.
  2. Biebl, H., Allgaier, M., Lünsdorf, H., Pukall, R., Tindall, B. J. & Wagner-Döbler, I.(2005).Roseovarius mucosus sp. nov., a member of the Roseobacter clade with trace amounts of bacteriochlorophyll a. Int J Syst Evol Microbiol 55, 2377–2383.[CrossRef] [Google Scholar]
  3. Boettcher, K. J., Geaghan, K. K., Maloy, A. P. & Barber, B. J.(2005).Roseovarius crassostreae sp. nov., a member of the Roseobacter clade and the apparent cause of juvenile oyster disease (JOD) in cultured Eastern oysters. Int J Syst Evol Microbiol 55, 1531–1537.[CrossRef] [Google Scholar]
  4. Bruns, A., Rohde, M. & Berthe-Corti, L.(2001).Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51, 1997–2006.[CrossRef] [Google Scholar]
  5. Cohen-Bazire, G., Sistrom, W. R. & Stanier, R. Y.(1957). Kinetic studies of pigment synthesis by nonsulfur purple bacteria. J Cell Comp Physiol 49, 25–68.[CrossRef] [Google Scholar]
  6. Cowan, S. T. & Steel, K. J.(1965).Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  7. Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef] [Google Scholar]
  8. González, J. M., Covert, J. S., Whitman, W. B., Henriksen, J. R., Mayer, F., Scharf, B., Schmitt, R., Buchan, A., Fuhrman, J. A. & other authors(2003).Silicibacter pomeroyi sp. nov. and Roseovarius nubinhibens sp. nov., dimethylsulfoniopropionate-demethylating bacteria from marine environments. Int J Syst Evol Microbiol 53, 1261–1269.[CrossRef] [Google Scholar]
  9. Komagata, K. & Suzuki, K.(1987). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207. [Google Scholar]
  10. Labrenz, M., Collins, M. D., Lawson, P. A., Tindall, B. J., Schumann, P. & Hirsch, P.(1999).Roseovarius tolerans gen. nov., sp. nov., a budding bacterium with variable bacteriochlorophyll a production from hypersaline Ekho Lake. Int J Syst Bacteriol 49, 137–147.[CrossRef] [Google Scholar]
  11. Lányí, B.(1987). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67. [Google Scholar]
  12. Leifson, E.(1963). Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85, 1183–1184. [Google Scholar]
  13. Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  14. Stackebrandt, E. & Goebel, B. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef] [Google Scholar]
  15. Staley, J. T.(1968).Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. J Bacteriol 95, 1921–1942. [Google Scholar]
  16. Tamaoka, J. & Komagata, K.(1984). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef] [Google Scholar]
  17. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  18. Yoon, J.-H., Kim, H., Kim, S.-B., Kim, H.-J., Kim, W. Y., Lee, S. T., Goodfellow, M. & Park, Y.-H.(1996). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46, 502–505.[CrossRef] [Google Scholar]
  19. Yoon, J.-H., Lee, S. T. & Park, Y.-H.(1998). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rRNA gene sequences. Int J Syst Bacteriol 48, 187–194.[CrossRef] [Google Scholar]
  20. Yoon, J.-H., Kang, K. H. & Park, Y.-H.(2003).Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53, 449–454.[CrossRef] [Google Scholar]
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