1887

Abstract

Three strains of anaerobic, variably pigmenting, Gram-negative bacilli isolated from human oral mucosal tissue were subjected to a comprehensive range of phenotypic and genotypic tests and were found to comprise a homogeneous group. 16S rRNA gene sequence analysis and DNA–DNA hybridization revealed that the strains constituted a novel group within the genus , being most closely related to and . A novel species, sp. nov., is proposed to accommodate these strains. is saccharolytic and produces acetic acid and succinic acid as major end products of fermentation and trace to minor amounts of isovaleric acid and lactic acid. The G+C content of the DNA of the type strain is 43 mol%. The type strain of is T05-04 (=DSM 19854=CCUG 55407).

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2008-08-01
2019-10-20
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References

  1. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. ( 1990; ). Basic local alignment search tool. J Mol Biol 215, 403–410.[CrossRef]
    [Google Scholar]
  2. Downes, J., Sutcliffe, I. C., Tanner, A. C. R. & Wade, W. G. ( 2005; ). Prevotella marshii sp. nov. and Prevotella baroniae sp. nov., isolated from the human oral cavity. Int J Syst Evol Microbiol 55, 1551–1555.[CrossRef]
    [Google Scholar]
  3. Hall, T. ( 2004; ). BioEdit. Carlsbad, CA: Ibis Therapeutics.
  4. Holdeman, L. V. H., Cato, E. P. & Moore, W. E. C. ( 1977; ). Anaerobe Laboratory Manual, 4th edn. Blacksburg, VA: Virginia Polytechnic Institute and State University.
  5. Hooper, S. J., Crean, S. J., Lewis, M. A., Spratt, D. A., Wade, W. G. & Wilson, M. J. ( 2006; ). Viable bacteria present within oral squamous cell carcinoma tissue. J Clin Microbiol 44, 1719–1725.[CrossRef]
    [Google Scholar]
  6. Huß, V. A. R., Festl, H. & Schleifer, K. H. ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef]
    [Google Scholar]
  7. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  8. Jousimies-Somer, H., Summanen, P., Citron, D. M., Baron, E. J., Wexler, H. M. & Finegold, S. M. ( 2002; ). Wadsworth Anaerobic Bacteriology Manual, 6th edn. Belmont, CA: Star Publishing.
  9. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  10. Rudney, J. D., Chen, R. & Zhang, G. ( 2005; ). Streptococci dominate the diverse flora within buccal cells. J Dent Res 84, 1165–1171.[CrossRef]
    [Google Scholar]
  11. Wade, W. G., Downes, J., Dymock, D., Hiom, S. J., Weightman, A. J., Dewhirst, F. E., Paster, B. J., Tzellas, N. & Coleman, B. ( 1999; ). The family Coriobacteriaceae: reclassification of Eubacterium exiguum (Poco et al. 1996) and Peptostreptococcus heliotrinreducens (Lanigan 1976) as Slackia exigua gen. nov., comb. nov. and Slackia heliotrinireducens gen. nov., comb. nov., and Eubacterium lentum (Prevot 1938) as Eggerthella lenta gen. nov., comb. nov. Int J Syst Bacteriol 49, 595–600.[CrossRef]
    [Google Scholar]
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Extended neighbour-joining tree, based on 16S rRNA gene sequence comparisons over 1415 aligned bases, showing the relationships between strains T05-04 , N19-30 and N12-20 ( sp. nov.) and related species. [PDF](18 KB)

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Characteristics of sp. nov. [PDF](19 KB)

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