1887

Abstract

A Gram-negative strain, B11, was isolated from tidal flat sediment in Yeosu, Republic of Korea. Strain B11 did not require NaCl for growth and grew between 18 and 44 °C. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain B11 was associated with the genus and was closely related to the type strain of (98.3 % similarity). Within the phylogenetic tree, the novel isolate shared a branching point with . Analysis of 16S rRNA gene sequences and DNA–DNA relatedness, as well as physiological and biochemical tests, indicated genotypic and phenotypic differences between strain B11 and the type strain of . Thus, strain B11 is proposed as a representative of a novel species, sp. nov.; the type strain is B11 (=KCTC 22051 =DSM 19476).

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2009-02-01
2019-12-05
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References

  1. Baker, G. C., Smith, J. J. & Cowan, D. A. ( 2003; ). Review and re-analysis of domain-specific 16S primers. J Microbiol Methods 55, 541–555.[CrossRef]
    [Google Scholar]
  2. Brettar, I., Christen, R. & Höfle, M. G. ( 2006; ). Rheinheimera perlucida sp. nov., a marine bacterium of the Gammaproteobacteria isolated from surface water of the central Baltic Sea. Int J Syst Evol Microbiol 56, 2177–2183.[CrossRef]
    [Google Scholar]
  3. Buck, J. D. ( 1982; ). Nonstaining (KOH) method for determination of gram reactions of marine bacteria. Appl Environ Microbiol 44, 992–993.
    [Google Scholar]
  4. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  5. Fonnesbech Vogel, B. F., Venkateswaran, K., Christensen, H., Falsen, E., Christiansen, G. & Gram, L. ( 2000; ). Polyphasic taxonomic approach in the description of Alishewanella fetalis gen. nov., sp. nov., isolated from a human foetus. Int J Syst Evol Microbiol 50, 1133–1142.[CrossRef]
    [Google Scholar]
  6. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  7. Kluge, A. G. & Farris, J. S. ( 1969; ). Quantitative phyletics and the evolution of anurans. Syst Zool 18, 1–32.[CrossRef]
    [Google Scholar]
  8. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  9. Mesbah, M. & Whitman, W. B. ( 1989; ). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. J Chromatogr 479, 297–306.[CrossRef]
    [Google Scholar]
  10. MIDI ( 1999; ). Sherlock Microbial Identification System Operating Manual, version 3.0. Newark, DE: MIDI Inc.
  11. Rice, P., Longden, I. & Bleasby, A. ( 2000; ). emboss: the European Molecular Biology Open Software Suite. Trends Genet 16, 276–277.[CrossRef]
    [Google Scholar]
  12. Roh, S. W., Sung, Y., Nam, Y. D., Chang, H. W., Kim, K. H., Yoon, J. H., Jeon, C. O., Oh, H. M. & Bae, J. W. ( 2008; ). Arthrobacter soli sp. nov., a novel bacterium isolated from wastewater reservoir sediment. J Microbiol 46, 40–44.[CrossRef]
    [Google Scholar]
  13. Romanenko, L. A., Uchino, M., Falsen, E., Zhukova, N. V., Mikhailov, V. V. & Uchimura, T. ( 2003; ). Rheinheimera pacifica sp. nov., a novel halotolerant bacterium isolated from deep sea water of the Pacific. Int J Syst Evol Microbiol 53, 1973–1977.[CrossRef]
    [Google Scholar]
  14. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  15. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark: DE: MIDI Inc.
  16. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Kreig. Washington, DC: American Society for Microbiology.
  17. Swofford, D. L. ( 1998; ). paup*: Phylogenetic analysis using parsimony (and other methods), version 4. Sunderland, MA: Sinauer Associates.
  18. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  19. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  20. Yoon, J. H., Bae, S. E., Kang, S. J. & Oh, T. K. ( 2007; ). Rheinheimera aquimaris sp. nov., isolated from seawater of the East Sea in Korea. Int J Syst Evol Microbiol 57, 1386–1390.[CrossRef]
    [Google Scholar]
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