A Gram-stain-positive, strictly anaerobic, mesophilic, amylolytic, rod-shaped bacterium, designated strain SW408, was isolated from a laboratory-scale H-producing upflow anaerobic sludge blanket reactor. The strain grew at 24–45 °C (no growth at or below 22 °C or at or above 47 °C), with optimum growth at 37 °C. The pH range for growth was 4.0–9.0 (no growth at or below pH 3.6 or at or above pH 9.3), with optimum growth at pH 7.0. Starch, cellobiose, glucose, fructose, galactose, lactose, maltose, mannose, ribose and sucrose supported growth. The major end products from glucose fermentation were ethanol, acetate, hydrogen and carbon dioxide. Abundant H was produced from starch fermentation. The DNA G+C content was 33.1 mol% ( method). Phylogenetic analysis based on 16S rRNA gene sequence analysis showed that the bacterium represents a previously unrecognized species within rRNA cluster I and is most closely related to the type strain of (94.9 % similarity). On the basis of phenotypic, genotypic and phylogenetic characteristics, strain SW408 was identified as a representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SW408 (=JCM 14823=AS 1.5069=CGMCC 1.5069).


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vol. , part 9, pp. 2132 - 2135

Electron micrograph of negatively stained cell of strain SW408 showing peritrichous flagella.

Differences in cellular fatty acid composition of strain SW408 and phylogenetically related clostridia.

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