1887

Abstract

The taxonomic status of the species , and was analysed in this study on the basis of their molecular and phenotypic characteristics. According to the results, the type strain ATCC 14482 does not belong to any of the already described species of the genus and it should therefore be considered as a different species. In contrast, the strains of examined belonged to and thus is a later synonym of . The results of the analysis of 16S–23S intergenic spacer region and , and gene sequences as well as those of DNA–DNA hybridization experiments and phenotypic characterizations showed that the type strains USDA 2370 and DSM 30132 do not belong to the same species. Taking into account that strain USDA 2370 corresponds to the original strain of this species, 3Hoq18, this strain should be considered as the true type strain of whereas strain DSM 30132 should be reclassified as a different species, for which the name sp. nov. is proposed (type strain, DSM 30132=NCIMB 11478).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65621-0
2008-11-01
2020-01-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/11/2484.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65621-0&mimeType=html&fmt=ahah

References

  1. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. ( 1990; ). Basic local alignment search tool. J Mol Biol 215, 403–410.[CrossRef]
    [Google Scholar]
  2. Bergersen, F. J. ( 1961; ). The growth of Rhizobium in synthetic media. Aust J Biol Sci 14, 349–360.
    [Google Scholar]
  3. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  4. Gaunt, M. W., Turner, S. L., Rigottier-Gois, L., Lloyd-Macgilp, S. A. & Young, J. W. P. ( 2001; ). Phylogenies of atpD and recA support the small subunit rRNA-based classification of rhizobia. Int J Syst Evol Microbiol 51, 2037–2048.[CrossRef]
    [Google Scholar]
  5. Jordan, D. C. ( 1984; ). Family III. Rhizobiaceae. In Bergey's Manual of Systematic Bacteriology, vol. I, pp. 234–242. Edited by N. R. Krieg & J. G. Holt. Baltimore: Williams and Wilkins Co.
  6. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  7. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M. ( 2001; ). Molecular Evolutionary Genetics Analysis Software. Tempe, AZ: Arizona State University.
  8. Kuykendall, L. D. ( 2005; ). Family I. Rhizobiaceae Conn 1938, 321AL. In Bergey's Manual of Systematic Bacteriology, vol. 2, part C, pp. 324–361. Edited by D. J. Brenner, N. R. Krieg & J. T. Stanley. New York, NY: Springer.
  9. Kwon, S. W., Park, J. Y., Kim, J. S., Kang, J. W., Cho, Y. H., Lim, C. K., Parker, M. A. & Lee, G. B. ( 2005; ). Phylogenetic analysis of the genera Bradyrhizobium, Mesorhizobium, Rhizobium and Sinorhizobium on the basis of 16S rRNA gene and internally transcribed spacer region sequences. Int J Syst Evol Microbiol 55, 263–270.[CrossRef]
    [Google Scholar]
  10. Rivas, R., Velázquez, E., Willems, A., Vizcaíno, N., Subba-Rao, N. S., Mateos, P. F., Gillis, M., Dazzo, F. B. & Martínez-Molina, E. ( 2002; ). A new species of Devosia that forms a nitrogen-fixing root-nodule symbiosis with the aquatic legume Neptunia natans (L. f.) Druce. Appl Environ Microbiol 68, 5217–5222.[CrossRef]
    [Google Scholar]
  11. Saitou, N. & Nei, M. ( 1987; ). The neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  12. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  13. Valverde, A., Igual, J. M., Peix, A., Cervantes, E. & Velázquez, E. ( 2006; ). Rhizobium lusitanum sp. nov. a bacterium that nodulates Phaseolus vulgaris. Int J Syst Evol Microbiol 56, 2631–2637.[CrossRef]
    [Google Scholar]
  14. van Berkum, P., Beyene, D. & Eardly, B. D. ( 1996; ). Phylogenetic relationships among Rhizobium species nodulating the common bean (Phaseolus vulgaris L.). Int J Syst Bacteriol 46, 240–244.[CrossRef]
    [Google Scholar]
  15. Velázquez, E., Igual, J. M., Willems, A., Fernández, M. P., Muñoz, E., Mateos, P. F., Abril, A., Toro, N., Normand, P. & other authors ( 2001a; ). Mesorhizobium chacoense sp. nov., a novel species that nodulates Prosopis alba in the Chaco Arido region (Argentina). Int J Syst Evol Microbiol 51, 1011–1021.[CrossRef]
    [Google Scholar]
  16. Velázquez, E., Martínez-Romero, E., Rodríguez-Navarro, D. N., Trujillo, M. E., Daza, A., Mateos, P. F., Martinez-Molina, E. & Van Berkum, P. ( 2001b; ). Characterization of rhizobial isolates of Phaseolus vulgaris by staircase electrophoresis of low molecular weight RNA. Appl Environ Microbiol 67, 1008–1010.[CrossRef]
    [Google Scholar]
  17. Vincent, J. M. ( 1970; ). The cultivation, isolation and maintenance of rhizobia. In A Manual for the Practical Study of Root-Nodule Bacteria, pp. 1–13. Edited by J. M. Vincent. Oxford: Blackwell Scientific.
  18. Willems, A., Doignon-Bourcier, F., Goris, J., Coopman, R., De Lajudie, P. & Gillis, M. ( 2001; ). DNA-DNA hybridization study of Bradyrhizobium strains. Int J Syst Evol Microbiol 51, 1315–1322.
    [Google Scholar]
  19. Zurdo-Piñeiro, J. L., Velázquez, E., Lorite, M. J., Brelles-Mariño, G., Schröder, E. C., Bedmar, E. J., Mateos, P. F. & Martínez-Molina, E. ( 2004; ). Identification of fast-growing rhizobia nodulating tropical legumes from Puerto Rico as Rhizobium gallicum and Rhizobium tropici. Syst Appl Microbiol 27, 469–477.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65621-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65621-0
Loading

Data & Media loading...

Supplements

vol. , part 11, pp. 2484 - 2490

Gene sequence similarities (%) among the strains analysed in this study and their closest relative . Results of DNA-DNA hybridization experiments. Means of DNA-DNA hybridization values.

Combined file [ PDF] 36 KB



PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error