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Abstract

A novel ovoid, phototrophic, purple non-sulfur bacterium was isolated in pure culture from a sample of sediment from an industrially polluted pond (Noor Mohammad Kunta) in Hyderabad, India. Strain JA234 was found to be Gram-negative and non-motile and grew photoheterotrophically with a number of organic compounds serving as carbon source/electron donor. Photo-organoheterotrophic and chemo-organoheterotrophic growth were demonstrated. Biotin and thiamine were required for growth of strain JA234. Phylogenetic analysis on the basis of 16S rRNA gene sequences showed that strain JA234 is most closely related to (97.3 % sequence similarity) and clusters with other species of the genus of the family . However, DNA–DNA hybridization with JCM 9340 showed a relatedness of only 31.3 % with respect to strain JA234. On the basis of 16S rRNA gene sequence analysis, DNA–DNA hybridization data and morphological and physiological characteristics, strain JA234 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JA234 (=JCM 14779=CCUG 55049).

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2008-06-01
2019-12-06
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References

  1. Anil Kumar, P., Srinivas, T. N. R., Sasikala, Ch. & Ramana, Ch. V. ( 2007; ). Rhodobacter changlensis sp. nov., a novel psychrophototrophic alphaproteobacterium from the Himalayas of India. Int J Syst Evol Microbiol 57, 2568–2571.[CrossRef]
    [Google Scholar]
  2. Cashion, P., Holder-Franklin, M. A., McCully, J. & Franklin, M. ( 1977; ). A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81, 461–466.[CrossRef]
    [Google Scholar]
  3. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  4. Felsenstein, J. ( 1989; ). phylip – phylogeny inference package (version 3.2). Cladistics 5, 164–166.
    [Google Scholar]
  5. Hanada, S., Takaichi, S., Matsuura, K. & Nakamura, K. ( 2002; ). Roseiflexus castenholzii gen. nov., sp. nov., a thermophilic, filamentous, photosynthetic bacterium that lacks chlorosomes. Int J Syst Evol Microbiol 52, 187–193.
    [Google Scholar]
  6. Hansen, T. A. & Imhoff, J. F. ( 1985; ). Rhodobacter veldkampii, a new species of phototrophic purple nonsulfur bacteria. Int J Syst Bacteriol 35, 115–116.[CrossRef]
    [Google Scholar]
  7. Hiraishi, A., Muramatsu, K. & Ueda, Y. ( 1996; ). Molecular genetic analysis of Rhodobacter azotoformans sp. nov. and related species of phototrophic bacteria. Syst Appl Microbiol 19, 168–177.[CrossRef]
    [Google Scholar]
  8. Huß, V. A. R., Festl, H. & Schleifer, K. H. ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef]
    [Google Scholar]
  9. Imhoff, J. F. ( 2005; ). Genus Rhodobacter Imhoff, Trüper and Pfennig 1984, 342VP. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, part C, pp. 161–167. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  10. Srinivas, T. N. R., Anil Kumar, P., Sasikala, Ch. & Ramana, Ch. V. ( 2007a; ). Rhodovulum imhoffii sp. nov. Int J Syst Evol Microbiol 57, 228–232.[CrossRef]
    [Google Scholar]
  11. Srinivas, T. N. R., Anil Kumar, P., Sasikala, Ch., Ramana, Ch. V. & Imhoff, J. F. ( 2007b; ). Rhodobacter vinaykumarii sp. nov., a marine phototrophic alphaproteobacterium from tidal waters, and emended description of the genus Rhodobacter. Int J Syst Evol Microbiol 57, 1984–1987.[CrossRef]
    [Google Scholar]
  12. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
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vol. , part 6, pp. 1379 - 1383

Electron microphotograph of an ultrathin section of a cell of strain JA234 showing the vesicular nature of the photosynthetic membranes. [PDF](51 KB)



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