1887

Abstract

A Gram-negative, coccoid to rod-shaped, strictly aerobic bacterium, strain CL-GR66, was isolated from coastal seawater collected off Korea. The strain grew optimally in the presence of 3–5 % sea salts, at a temperature of 30 °C and at pH 7. The polar lipid profile of strain CL-GR66 comprised phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, two unidentified aminolipids, an unidentified glycolipid and an unidentified lipid. The major fatty acids were C 7 (71.4 %), 11-methyl C 7 (11.3 %), C (6.4 %) C (4.6 %) and C 3-OH (3.0 %). Ubiquinone 10 was the major quinone. The DNA G+C content was 67.9 mol%. 16S rRNA gene sequence analysis revealed that strain CL-GR66 belonged to the clade within the family . Strain CL-GR66 was related most closely to the type strain of (95.5 % 16S rRNA gene sequence similarity), and sequence similarities between strain CL-GR66 and other type species of the clade ranged from 91.8 to 95.4 %. Phylogenetic analyses of 16S rRNA gene sequences showed that strain CL-GR66 was not associated with any known genus in the family . The distinct phylogenetic position of strain CL-GR66, its phylogenetic distance from genera of the family , together with phenotypic data, suggest that the organism represents a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is CL-GR66 (=KCCM 90028 =DSM 18986).

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2008-06-01
2020-01-24
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Transmission electron micrograph of negatively stained cells of strain CL-GR66 . Bar, 1 µm.

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Two-dimensional thin-layer chromatogram of the polar lipids of strain CL-GR66 . Total polar lipids were stained with 5% ethanolic molybdophosphoric acid. Solvents: chloroform/methanol/water (65:25:4 by vol.), first direction; chloroform/methanol/acetic acid/water (80:12:15:4 by vol.), second direction. 1, Phosphatidylcholine; 2, unidentified glycolipid; 3, phosphatidylglycerol; 4, unidentified aminolipid I; 5, phosphatidylethanolamine; 6, unidentified aminolipid II; 7, unidentified lipid.

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Neighbour-joining tree derived from 16S rRNA gene sequences for strain CL-GR66 and most members of the family . [PDF](29 KB)

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Cellular fatty acid compositions of strain CL-GR66 and other phylogenetically related genera in the family . [PDF](136 KB)

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