1887

Abstract

A Gram-negative, aerobic, rod-shaped bacterium, designated strain JA3-B52, was isolated from the roots of fresh rice plants (). The cells were motile by means of polar single or lateral flagella. The colonies were non-pigmented. On the basis of 16S rRNA gene sequence comparisons, the strain was phylogenetically related to species of the genus , having the greatest level of sequence similarity with respect to A62-14B (97.16 %). The bacterium grew at temperatures from 10 to 37 °C, with an optimum at 30 °C. The strain exhibited growth with 0–3.0 % (w/v) NaCl and at pH 6.0–8.5. The main cellular fatty acids were C 7, C 8, C , C 7 and C 3-OH. The DNA G+C content was 47.0 mol%. The levels of similarity between the 16S rRNA gene sequence of strain JA3-B52 and those of the type strains of species ranged from 95.38 to 97.16 %. The mean level of DNA–DNA relatedness between strain JA3-B52 and A62-14B, the strain most closely related to the isolate, was 20.4 %. On the basis of physiological and biochemical characteristics and genotypic data obtained in this work, strain JA3-B52 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JA3-B52 (=CGMCC 1.6362 =DSM 19460).

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2008-10-01
2019-09-22
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References

  1. Brettar, I., Christen, R. & Höfle, M. G. ( 2002; ). Rheinheimera baltica gen. nov., sp. nov., a blue-coloured bacterium isolated from the central Baltic Sea. Int J Syst Evol Microbiol 52, 1851–1857.[CrossRef]
    [Google Scholar]
  2. Brettar, I., Christen, R. & Höfle, M. G. ( 2006; ). Rheinheimera perlucida sp. nov., a marine bacterium of the Gammaproteobacteria isolated from surface water of the central Baltic Sea. Int J Syst Evol Microbiol 56, 2177–2183.[CrossRef]
    [Google Scholar]
  3. Dong, X.-Z. & Cai, M.-Y. ( 2001; ). Determinative Manual for Routine Bacteriology. Beijing: Scientific Press (English translation).
  4. Dong, X., Xin, Y., Jian, W., Liu, X. & Ling, D. ( 2000; ). Bifidobacterium thermacidophilum sp. nov., isolated from an anaerobic digester. Int J Syst Evol Microbiol 50, 119–125.[CrossRef]
    [Google Scholar]
  5. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  6. Fonnesbech Vogel, B., Venkateswaran, K., Christensen, H., Falsen, E., Christiansen, G. & Gram, L. ( 2000; ). Polyphasic taxonomic approach in the description of Alishewanella fetalis gen. nov., sp. nov., isolated from a human foetus. Int J Syst Evol Microbiol 50, 1133–1142.[CrossRef]
    [Google Scholar]
  7. Halpern, M., Senderovich, Y. & Snir, S. ( 2007; ). Rheinheimera chironomi sp. nov., isolated from a chironomid (Diptera; Chironomidae) egg mass. Int J Syst Evol Microbiol 57, 1872–1875.[CrossRef]
    [Google Scholar]
  8. Kämpfer, P. & Kroppenstedt, R. M. ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef]
    [Google Scholar]
  9. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitution through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  10. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  11. Lake, J. A. ( 1987; ). A rate-independent technique for analysis of nucleic acid sequences: evolutionary parsimony. Mol Biol Evol 4, 167–191.
    [Google Scholar]
  12. Lane, D. J. ( 1991; ). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. Chichester: Wiley.
  13. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  14. Marmur, J. & Doty, P. ( 1962; ). Determination of base composition of deoxyribonucleic acid from its denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  15. Merchant, M. M., Welsh, A. K. & McLean, R. J. C. ( 2007; ). Rheinheimera texasensis sp. nov., a halointolerant freshwater oligotroph. Int J Syst Evol Microbiol 57, 2376–2380.[CrossRef]
    [Google Scholar]
  16. Romanenko, L. A., Uchino, M., Falsen, E., Zhukova, N. V., Mikhailov, V. V. & Uchimura, T. ( 2003; ). Rheinheimera pacifica sp. nov., a novel halotolerant bacterium isolated from deep sea water of the Pacific. Int J Syst Evol Microbiol 53, 1973–1977.[CrossRef]
    [Google Scholar]
  17. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  18. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: Microbial MIDI Inc.
  19. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  20. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  21. Yoon, J. H., Park, S. E., Kang, S. J. & Oh, T. K. ( 2007; ). Rheinheimera aquimaris sp. nov., isolated from seawater of the East Sea in Korea. Int J Syst Evol Microbiol 57, 1386–1390.[CrossRef]
    [Google Scholar]
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