1887

Abstract

A marine, Gram-negative, deep-orange-coloured, rod/coccoid-shaped, strictly aerobic, non-motile, oxidase- and catalase-positive bacterial strain, designated YM6-073, was isolated from sediment collected in Okinawa, Japan. Strain YM6-073 produced the carotenoid (3,2′)-myxol. A phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YM6-073 belonged to a distinct lineage in the family , the closest relative being HTCC2501 (93.7 % sequence similarity). Strain YM6-073 could be distinguished from other members of the family by means of a number of phenotypic characteristics. The DNA G+C content was 50.1 mol%. The predominant cellular fatty acids were iso-C, iso-C 10, iso-C 3-OH and C and menaquinone-6 was the only respiratory quinone. DNA–DNA hybridization between strain YM6-073 and DSM 15991 showed less than 10 % DNA–DNA relatedness. On the basis of phenotypic, genotypic and phylogenetic features, strain YM6-073 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YM6-073 (=MBIC 06409=KCTC 22216=DSM 21019).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65598-0
2008-07-01
2019-12-11
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/7/1660.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65598-0&mimeType=html&fmt=ahah

References

  1. Bernardet, J.-F. & Nakagawa, Y. ( 2006; ). An introduction to the family Flavobacteriaceae. In The Prokaryotes, a Handbook on the Biology of Bacteria, 3rd edn, vol. 7, pp. 455–480. Edited by M. Dworkin, S. Falkow, E. Rosenberg, K.-H. Schleifer & E. Stackebrandt. New York: Springer.
  2. Bernardet, J.-F., Nakagawa, Y. & Holmes, B. ( 2002; ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52, 1049–1070.[CrossRef]
    [Google Scholar]
  3. Bowman, J. P. ( 2006; ). The marine clade of the family Flavobacteriaceae: the genera Aequorivita, Arenibacter, Cellulophaga, Croceibacter, Formosa, Gelidibacter, Gillisia, Maribacter, Mesonia, Muricauda, Polaribacter, Psychroflexus, Psychroserpens, Robiginitalea, Salegentibacter, Tenacibaculum, Ulvibacter, Vitellibacter, and Zobellia. In The Prokaryotes, a Handbook on the Biology of Bacteria, 3rd edn, vol. 7, pp. 677–694. Edited by M. Dworkin, S. Falkow, E. Rosenberg, K.-H. Schleifer & E. Stackebrandt. New York: Springer.
  4. Cho, J.-C. & Giovannoni, S. J. ( 2004; ). Robiginitalea biformata gen. nov., sp. nov., a novel marine bacterium in the family Flavobacteriaceae with a higher G+C content. Int J Syst Evol Microbiol 54, 1101–1106.[CrossRef]
    [Google Scholar]
  5. Cole, J. R., Chai, B., Farris, R. J., Wang, Q., Kulam-Syed-Mohideen, A. S., McGarrell, D. M., Bandela, A. M., Cardenas, E., Garrity, G. M., Tiedje, J. M. & other authors ( 2007; ). The ribosomal database project (RDP-II): introducing myRDP space and quality controlled public data. Nucleic Acids Res 35, (Database issue). D169–D172.[CrossRef]
    [Google Scholar]
  6. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  7. Felsenstein, J. ( 1993; ). phylip (phylogeny inference package), version 3.5. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  8. Garrity, G. M. & Holt, J. G. ( 2001; ). The road map to the Manual. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, pp. 119–166. Edited by D. R. Boone, R. W. Castenholz & G. M. Garrity. New York: Springer.
  9. Guindon, S., Lethiec, F., Duroux, P. & Gascuel, O. ( 2005; ). PHYML Online–a web server for fast maximum likelihood-based phylogenetic inference. Nucleic Acids Res 33, (Web Server issue). W557–W559.[CrossRef]
    [Google Scholar]
  10. Ivanova, E. P., Nedashkovskaya, O. I., Chun, J., Lysenko, A. M., Frolova, G. M., Svetashev, V. I., Vysotskii, M. V., Mikhailov, V. V., Huq, A. & Colwell, R. R. ( 2001; ). Arenibacter gen. nov., new genus of the family Flavobacteriaceae and description of a new species, Arenibacter latericius sp. nov. Int J Syst Evol Microbiol 51, 1987–1995.[CrossRef]
    [Google Scholar]
  11. Katsuta, A., Adachi, K., Matsuda, S., Shizuri, Y. & Kasai, H. ( 2005; ). Ferrimonas marina sp. nov. Int J Syst Evol Microbiol 55, 1851–1855.[CrossRef]
    [Google Scholar]
  12. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  13. Llobet-Brossa, E., Rosselló-Mora, R. & Amann, R. ( 1998; ). Microbial community composition of Wadden Sea sediments as revealed by fluorescence in situ hybridization. Appl Environ Microbiol 64, 2691–2696.
    [Google Scholar]
  14. Mesbah, M. & Whitman, W. B. ( 1989; ). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. J Chromatogr 479, 297–306.[CrossRef]
    [Google Scholar]
  15. Murray, R. G. E., Doetsch, R. N. & Robinow, F. ( 1994; ). Determinative and cytological light microscopy. In Methods for General and Molecular Bacteriology, pp. 21–41. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  16. O'Brien, M. & Colwell, R. R. ( 1987; ). A rapid test for chitinase activity that uses 4-methylumbelliferyl-N-acetyl-β-d-glucosaminide. Appl Environ Microbiol 53, 1718–1720.
    [Google Scholar]
  17. Shindo, K., Kikuta, K., Suzuki, A., Katsuta, A., Kasai, H., Yasumoto-Hirose, M., Matsuo, Y., Misawa, N. & Takaichi, S. ( 2007; ). Rare carotenoids, (3R)-saproxanthin and (3R,2′S)-myxol, isolated from novel marine bacteria (Flavobacteriaceae) and their antioxidative activities. Appl Microbiol Biotechnol 74, 1350–1357.[CrossRef]
    [Google Scholar]
  18. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  19. Weisburg, W. G., Barns, S. M., Pelletier, D. A. & Lane, D. J. ( 1991; ). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173, 697–703.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65598-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65598-0
Loading

Data & Media loading...

Supplements

Unrooted neighbour-joining phylogenetic tree based on the 16S rRNA gene sequences of strain YM6-073 and related organisms. [ PDF] 27 KB

PDF

Detailed compositions of the isolation media used in this study. [ PDF] 32 KB

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error