Three actinomycete strains, MC5-1, MC7-1 and R1-1, were isolated from soil samples collected in Thailand. Their taxonomic positions were determined using a polyphasic approach. The chemotaxonomic characteristics of these strains coincided with those of the genus , i.e. -diaminopimelic acid and glycolyl muramic acid were present in the cell-wall peptidoglycan, the whole-cell sugars were of pattern D, the phospholipids were of type II and the cellular fatty acids were of type 3b. Phylogenetic analysis of the 16S rRNA gene sequences revealed a close relationship between strains MC5-1, MC7-1 and R1-1 (99.8 % sequence similarity) and JCM 12357 (99.3 %). The three novel strains were clearly distinguishable from JCM 12357 from the low DNA–DNA relatedness (≤43.4 %). On the basis of the data presented, strain MC5-1 represents a novel species of the genus , for which the name is proposed. The type strain is MC5-1 (=KCTC 19332=JCM 12873=PCU 267=TISTR 1564).


Article metrics loading...

Loading full text...

Full text loading...



  1. Ara, I. & Kudo, T.(2007). Two new species of the genus Micromonospora: Micromonospora chokoriensis sp. nov. and Micromonospora coxensis sp. nov., isolated from sandy soil. J Gen Appl Microbiol 53, 29–37.[CrossRef] [Google Scholar]
  2. Arai, T.(1975).Culture Media for Actinomycetes. Tokyo: The Society for Actinomycetes, Japan.
  3. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E.(1977). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef] [Google Scholar]
  4. Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef] [Google Scholar]
  5. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  6. Gordon, R. E., Barnett, D. A., Handerhan, J. E. & Pang, C. H.-N.(1974).Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24, 54–63.[CrossRef] [Google Scholar]
  7. Hirsch, P., Mevs, U., Kroppenstedt, R. M., Schumann, P. & Stackebrandt, E.(2004). Crytoendolithic actinomycetes from Antarctic sandstone rock samples: Micromonospora endolithica sp. nov. and two isolates related to Micromonospora coerulea Jensen 1932. Syst Appl Microbiol 27, 166–174.[CrossRef] [Google Scholar]
  8. Itoh, T., Kudo, T., Parenti, F. & Seino, A.(1989). Amended description of the genus Kineosporia, based on chemotaxonomic and morphological studies. Int J Syst Bacteriol 39, 168–173.[CrossRef] [Google Scholar]
  9. Jacobson, E., Grauville, W. C. & Fogs, C. E.(1958).Color Harmony Manual, 4th edn. Chicago: Container Corporation of America.
  10. Kämpfer, P. & Kroppenstedt, R. M.(1996). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef] [Google Scholar]
  11. Kasai, H., Tamura, T. & Harayama, S.(2000). Intrageneric relationships among Micromonospora species deduced from gyrB-based phylogeny and DNA relatedness. Int J Syst Evol Microbiol 50, 127–134.[CrossRef] [Google Scholar]
  12. Kawamoto, I.(1989). Genus Micromonospora Ørskov 1923, 147AL. In Bergey's Manual of Systematic Bacteriology, vol. 4, pp. 2442–2450. Edited by S. T. Williams, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  13. Kawamoto, I., Oka, T. & Nara, T.(1981). Cell wall composition of Micromonospora olivasterospora, Micromonospora sagamiensis, and related organisms. J Bacteriol 146, 527–534. [Google Scholar]
  14. Kroppenstedt, R. M.(1985). Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics (Society for Applied Bacteriology Technical Series vol. 20), pp. 173–199. Edited by M. Goodfellow & D. E. Minnikin. New York: Academic Press.
  15. Kroppenstedt, R. M., Mayilraj, S., Wink, J. M., Kallow, W., Schumann, P., Secondini, C. & Stackebrandt, E.(2005). Eight new species of the genus Micromonospora, Micromonospora citrea sp. nov., Micromonospora echinaurantiaca sp. nov., Micromonospora echinofusca sp. nov. Micromonospora fulviviridis sp. nov., Micromonospora inyonensis sp. nov., Micromonospora peucetia sp. nov., Micromonospora sagamiensis sp. nov., and Micromonospora viridifaciens sp. nov. Syst Appl Microbiol 28, 328–339.[CrossRef] [Google Scholar]
  16. Kudo, T., Itoh, T., Miyadoh, S., Shomura, T. & Seino, A.(1993).Herbidospora gen. nov., a new genus of the family Streptosporangiaceae Goodfellow et al. 1990. Int J Syst Bacteriol 43, 319–328.[CrossRef] [Google Scholar]
  17. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  18. Lechevalier, M. P. & Lechevalier, H.(1970). Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20, 435–443.[CrossRef] [Google Scholar]
  19. Lechevalier, M. P., De Bièvre, C. & Lechevalier, H. A.(1977). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5, 249–260.[CrossRef] [Google Scholar]
  20. Mikami, H. & Ishida, Y.(1983). Post-column fluorometric detection of reducing sugars in high-performance liquid chromatography using arginine. Bunseki Kagaku 32, E207–E210.[CrossRef] [Google Scholar]
  21. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H.(1984). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef] [Google Scholar]
  22. Nakajima, Y., Kitpreechavanich, V., Suzuki, K. & Kudo, T.(1999).Microbispora corallina sp. nov., a new species of the genus Microbispora isolated from Thai soil. Int J Syst Bacteriol 49, 1761–1767.[CrossRef] [Google Scholar]
  23. Ørskov, J.(1923).Investigations into the Morphology of the Ray Fungi. Copenhagen: Levin & Munksgaard.
  24. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  25. Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  26. Shirling, E. B. & Gottlieb, D.(1966). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef] [Google Scholar]
  27. Staneck, J. L. & Roberts, G. D.(1974). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226–231. [Google Scholar]
  28. Stevenson, I. L.(1967). Utilization of aromatic hydrocarbons by Arthrobacter spp. Can J Microbiol 13, 205–211.[CrossRef] [Google Scholar]
  29. Suzuki, K. & Komagata, K.(1983). Taxonomic significance of cellular fatty acid composition in some coryneform bacteria. Int J Syst Bacteriol 33, 188–200.[CrossRef] [Google Scholar]
  30. Tamaoka, J.(1994). Determination of DNA base composition. In Chemical Methods in Prokaryotic Systematics, pp. 463–470. Edited by M. Goodfellow. & A. G. O'Donnell. Chichester: Wiley.
  31. Tamaoka, J. & Komagata, K.(1984). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef] [Google Scholar]
  32. Thawai, C., Tanasupawat, S., Itoh, T., Suwanborirux, K. & Kudo, T.(2004).Micromonospora auratinigra sp. nov., isolated from a peat swamp forest in Thailand. Actinomycetologica 18, 8–14.[CrossRef] [Google Scholar]
  33. Thawai, C., Tanasupawat, S., Itoh, T., Suwanborirux, K. & Kudo, T.(2005a).Micromonospora eburnea sp. nov., isolated from a Thai peat swamp forest. Int J Syst Evol Microbiol 55, 417–422.[CrossRef] [Google Scholar]
  34. Thawai, C., Tanasupawat, S., Itoh, T., Suwanborirux, K. & Kudo, T.(2005b).Micromonospora siamensis sp. nov., isolated from Thai peat swamp forest. J Gen Appl Microbiol 51, 229–234.[CrossRef] [Google Scholar]
  35. Thompson, J. D., Higgins, D. G. & Gibson, T. J.(1994).clustalw: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef] [Google Scholar]
  36. Trujillo, M. E., Fernândez-Molinero, C., Velâzquez, E., Kroppenstedt, R. M., Schumann, P., Mateos, P. F. & Martínez-Molina, E.(2005).Micromonospora mirobrigensis sp. nov. Int J Syst Evol Microbiol 55, 877–880.[CrossRef] [Google Scholar]
  37. Trujillo, M. E., Kroppenstedt, R. M., Schumann, P., Carro, L. & Martínez-Molina, E.(2006).Micromonospora coriariae sp. nov., isolated from root nodules of Coriaria myrtifolia. Int J Syst Evol Microbiol 56, 2381–2385.[CrossRef] [Google Scholar]
  38. Uchida, K. & Aida, K.(1984). An improved method for the glycolate test for simple identification of acyl type of bacterial cell walls. J Gen Appl Microbiol 30, 131–134.[CrossRef] [Google Scholar]
  39. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  40. Williams, S. T. & Cross, T.(1971). Actinomycetes. Methods Microbiol 4, 295–334. [Google Scholar]

Data & Media loading...


Combined file [ PDF] 39 KB




Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error