1887

Abstract

A psychrotolerant bacterium, designated strain Tibet-IIU11, was isolated from the Qinghai--Tibet Plateau permafrost region, China. A taxonomic study was conducted using a polyphasic approach, with determination of physiological and biochemical properties and phylogenetic analysis based on 16S rRNA gene sequences. The novel isolate was found to belong to the genus and was distinct from the recognized species of this genus. The major fatty acids were iso-C (24.5 %), summed feature 3 (C 7 and/or iso C 2-OH; 20.0 %), summed feature 4 (iso-C I and/or anteiso-C B; 14.0 %), C 5 (12.7 %) and anteiso-C (8.4 %). Phosphatidylethanolamine and an unknown aminophospholipid were predominant in the polar lipid profile. The quinone system consisted exclusively of menaquinone MK-7 and -homospermidine was the major polyamine present. These chemotaxonomic traits are in good agreement with the characteristics of the genus . The assignment of the novel isolate to this genus was further supported by a DNA G+C content of 60 mol%. On the basis of the polyphasic evidence presented in this study, it is proposed that strain Tibet-IIU11 (=DSM 18569=CGMCC 1.6365) represents a novel species, s sp. nov.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65588-0
2008-05-01
2019-08-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/5/1215.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65588-0&mimeType=html&fmt=ahah

References

  1. Altenburger, P., Kämpfer, P., Makristathis, A., Lubitz, W. & Busse, H.-J. ( 1996; ). Classification of bacteria isolated from a medieval wall painting. J Biotechnol 47, 39–52.[CrossRef]
    [Google Scholar]
  2. Baik, K. S., Seong, C. N., Moon, E. Y., Park, Y.-D., Yi, H. & Chun, J. ( 2006; ). Hymenobacter rigui sp. nov., isolated from wetland freshwater. Int J Syst Evol Microbiol 56, 2189–2192.[CrossRef]
    [Google Scholar]
  3. Buczolits, S., Denner, E. B. M., Kämpfer, P. & Busse, H.-J. ( 2006; ). Proposal of Hymenobacter norwichensis sp. nov., classification of ‘Taxeobacter ocellatus’, ‘Taxeobacter gelupurpurascens’ and ‘Taxeobacter chitinovorans’ in the genus Hymenobacter as Hymenobacter ocellatus sp. nov., Hymenobacter gelipurpurascens sp. nov. and Hymenobacter chitinivorans sp. nov., respectively, and emended description of the genus Hymenobacter Hirsch et al. 1999. Int J Syst Evol Microbiol 56, 2071–2078.[CrossRef]
    [Google Scholar]
  4. Buczolits, S., Denner, E. B. M., Vybiral, D., Wieser, M., Kämpfer, P. & Busse, H.-J. ( 2002; ). Classification of three airborne bacteria and proposal of Hymenobacter aerophilus sp. nov. Int J Syst Evol Microbiol 52, 445–456.
    [Google Scholar]
  5. Busse, H. J. & Auling, G. ( 1988; ). Polyamine pattern as a chemotaxonomic marker within the Proteobacteria. Syst Appl Microbiol 11, 1–8.[CrossRef]
    [Google Scholar]
  6. Cole, J. R., Chai, B., Marsh, T. L., Farris, R. J., Wang, Q., Kulam, S. A., Chandra, S., McGarrell, D. M., Schmidt, T. M. & other authors ( 2003; ). The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy. Nucleic Acids Res 31, 442–443.[CrossRef]
    [Google Scholar]
  7. Collins, M. D., Hutson, R. A., Grant, I. R. & Patterson, M. F. ( 2000; ). Phylogenetic characterization of a novel radiation-resistant bacterium from irradiated pork: description of Hymenobacter actinosclerus sp. nov. Int J Syst Evol Microbiol 50, 731–734.[CrossRef]
    [Google Scholar]
  8. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  9. Hirsch, P., Ludwig, W., Hethke, C., Sittig, M., Hoffmann, B. & Gallikowski, C. A. ( 1998; ). Hymenobacter roseosalivarius gen. nov., sp. nov. from continental Antarctic soils and sandstone: bacteria of Cytophaga/Flavobacterium/Bacteroides line of phylogenetic descent. Syst Appl Microbiol 21, 374–383.[CrossRef]
    [Google Scholar]
  10. Kämpfer, P., Steiof, M. & Dott, W. ( 1991; ). Microbiological characterization of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. Microb Ecol 21, 227–251.[CrossRef]
    [Google Scholar]
  11. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M. ( 2001; ). mega2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef]
    [Google Scholar]
  12. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  13. Moaledj, K. ( 1986; ). Comparison of Gram-staining and alternate methods, KOH test and aminopeptidase activity in aquatic bacteria: their application to numerical taxonomy. J Microbiol Methods 5, 303–310.[CrossRef]
    [Google Scholar]
  14. Porter, K. & Feig, Y. S. ( 1990; ). The use of DAPI for identifying and counting aquatic microflora. Limnol Oceanogr 25, 943–948.
    [Google Scholar]
  15. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  16. Sambrook, J., Frisch, E. F. & Maniatis, T. ( 1989; ). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  17. Sherr, B. F., Sherr, E. B. & McDaniel, J. ( 1992; ). Effect of protistan grazing on the frequency of dividing cells in bacterioplankton assemblages. Appl Environ Microbiol 58, 2381–2385.
    [Google Scholar]
  18. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterisation. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Woods & N. R. Krieg. Washington, DC: American Society for Microbiology.
  19. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  20. Staley, J. T. ( 1968; ). Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. J Bacteriol 95, 1921–1942.
    [Google Scholar]
  21. Stolz, A., Busse, H.-J. & Kämpfer, P. ( 2007; ). Pseudomonas knackmussii sp. nov. Int J Syst Evol Microbiol 57, 572–576.[CrossRef]
    [Google Scholar]
  22. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  23. Tindall, B. J. ( 1990a; ). Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 66, 199–202.[CrossRef]
    [Google Scholar]
  24. Tindall, B. J. ( 1990b; ). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13, 128–130.[CrossRef]
    [Google Scholar]
  25. Wang, B. & French, H. M. ( 1995; ). Permafrost on the Tibet Plateau, China. Quatern Sci Rev 14, 255–274.[CrossRef]
    [Google Scholar]
  26. Zhang, G., Ma, X., Niu, F., Dong, M., Feng, H., An, L. & Cheng, G. ( 2007a; ). Diversity and distribution of alkaliphilic psychrotolerant bacteria in the Qinghai-Tibet Plateau permafrost region. Extremophiles 11, 415–424.[CrossRef]
    [Google Scholar]
  27. Zhang, G., Niu, F., Ma, X., Liu, W., Dong, M., Feng, H., An, L. & Cheng, G. ( 2007b; ). Phylogenetic diversity of bacteria isolates from the Qinghai-Tibet Plateau permafrost region. Can J Microbiol 53, 1000–1010.[CrossRef]
    [Google Scholar]
  28. Zhang, Q., Liu, C., Tang, Y., Zhou, G., Shen, P., Fang, C. & Yokota, A. ( 2007c; ). Hymenobacter xinjiangensis sp. nov., a radiation-resistant bacterium isolated from the desert of Xinjiang, China. Int J Syst Evol Microbiol 57, 1752–1756.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65588-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65588-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error