A high-throughput screening effort, designed to isolate bacteriocin-producing lactic acid bacteria (LAB) from malted cereals, resulted in the isolation of four bacteriocin-producing strains that could not be assigned conclusively to recognized species. The four isolates (UCC128, UCC125, UCC126 and UCC127) were found to share identical (100 %) 16S rRNA gene sequences and were therefore deemed to belong to the same species. The strains were Gram-positive, catalase-negative, non-motile homofermentative LAB. The closest recognized relative to strain UCC128 identified based on comparative 16S rRNA gene sequence analysis was DSM 20444 (97 % similarity). The strains were characterized phenotypically to identify specific growth requirements. DNA–DNA hybridization between strain UCC128 and DSM 20444 revealed a level of relatedness of only 39.4 %. This indicates that strain UCC128 does not belong to the species . The four bacteriocin-producing strains are therefore considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is UCC128 (=DSM 19519=LMG 24241).


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vol. , part 9, pp. 2013 - 2017

Phylogenetic relationship of sp. nov. to related species of the genus based on 16S rRNA gene sequences, constructed using the maximum-parsimony method.

. Phylogenetic relationship of sp. nov. to related species of the genus based on 16S rRNA gene sequences, constructed using the maximum likelihood method.

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