A Gram-negative, non-spore-forming, cream–yellow-pigmented bacterial strain, IP-10, was isolated from soil samples from a waste site highly contaminated with hexachlorocyclohexane in Ummari village, India. The organism showed the highest 16S rRNA gene sequence similarity of 92.7 % with KCTC 12542 and levels of 87–92 % with the type strains of other recognized species of the genus . The DNA G+C content of strain IP-10 was 31 mol%. The predominant fatty acids were iso-C (22.1 %), iso-C 3-OH (18.5 %) and summed feature 3 (comprising C 7 and/or iso-C 2-OH; 13.2 %). Strain IP-10 could be differentiated from recognized species of the genus based on a number of phenotypic features. Strain IP-10 is therefore considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is IP-10 (=MTCC 8597=CCM 7424).


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  1. Anacker, R. L. & Ordal, E. J.(1955). Study of a bacteriophage infecting the myxobacterium Chondrococcus columnaris. J Bacteriol 70, 738–741. [Google Scholar]
  2. Arden-Jones, M. P., McCarthy, A. J. & Cross, T.(1979). Taxonomic and serological studies on Micropolyspora faeni and Micropolyspora strains from soil bearing the specific epithet rectivirgula. J Gen Microbiol 115, 343–354.[CrossRef] [Google Scholar]
  3. Bergey, D. H., Harrison, F. C., Breed, R. S., Hammer, B. W. & Huntoon, F. M.(1923). Genus II. Flavobacterium gen. nov. In Bergey's Manual of Determinative Bacteriology, pp. 97–117. Baltimore: Williams & Wilkins.
  4. Bernardet, J.-F. & Bowman, J.(2006). The genus Flavobacterium. In The Prokaryotes: A Handbook on the Biology of Bacteria, 3rd edn, vol. 7, pp. 481–531. Edited by M. Dworkin, S. Falkow, E. Rosenberg, K. H. Schleifer & E. Stackebrandt. New York: Springer.
  5. Bernardet, J. F., Segers, P., Vancanneyt, M., Berthe, F., Kersters, K. & Vandamme, P.(1996). Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46, 128–148.[CrossRef] [Google Scholar]
  6. Bernardet, J.-F., Nakagawa, Y. & Holmes, B.(2002). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52, 1049–1070.[CrossRef] [Google Scholar]
  7. Christensen, W. B.(1946). Urea decomposition as a means of differentiating Proteus and Paracolon cultures from each other and from Salmonella and Shigella types. J Bacteriol 52, 461–466. [Google Scholar]
  8. Chun, J., Lee, J.-H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y. W.(2007). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef] [Google Scholar]
  9. Cowan, S. T. & Steel, K. J.(1965).Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  10. Felsenstein, J.(1993).phylip (phylogeny inference package), version 3.5c. Department of Genome Sciences, University of Washington, Seattle, USA.
  11. Gonzalez, J. M. & Saiz-Jimenez, C.(2002). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 4, 770–773.[CrossRef] [Google Scholar]
  12. Hugh, R. & Leifson, E.(1953). The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram negative bacteria. J Bacteriol 66, 24–26. [Google Scholar]
  13. Humphry, D. R., George, A., Black, G. W. & Cummings, S. P.(2001).Flavobacterium frigidarium sp. nov., an aerobic, psychrophilic, xylanolytic and laminarinolytic bacterium from Antarctica. Int J Syst Evol Microbiol 51, 1235–1243. [Google Scholar]
  14. Jukes, T. H. & Cantor, C. R.(1969). Evolution of protein molecules. In Mammalian Protein Metabolism, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  15. Kim, B. Y., Weon, H. Y., Cousin, S., Yoo, S. H., Kwon, S. W., Go, S. J. & Stackebrandt, E.(2006).Flavobacterium daejeonense sp. nov. and Flavobacterium suncheonense sp. nov., isolated from greenhouse soils in Korea. Int J Syst Evol Microbiol 56, 1645–1649.[CrossRef] [Google Scholar]
  16. Kuykendall, L. D., Roy, M. A., O'Neill, J. J. & Devine, T. E.(1988). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum. Int J Syst Bacteriol 38, 358–361.[CrossRef] [Google Scholar]
  17. McCarthy, A. J. & Cross, T.(1984). A taxonomic study of Thermomonospora and other monosporic actinomycetes. J Gen Microbiol 130, 5–25. [Google Scholar]
  18. Miller, L. T.(1982). Single derivatization method for the routine analysis of whole-cell fatty acid methyl esters, including hydroxyl acids. J Clin Microbiol 16, 584–586. [Google Scholar]
  19. Park, M., Lu, S., Ryu, S. H., Chung, B. S., Park, W., Kim, C.-J. & Jeon, C. O.(2006).Flavobacterium croceum sp. nov., isolated from activated sludge. Int J Syst Evol Microbiol 56, 2443–2447.[CrossRef] [Google Scholar]
  20. Prakash, O., Kumari, K. & Lal, R.(2007).Pseudomonas delhiensis sp. nov., from a fly ash dumping site of a thermal power plant. Int J Syst Evol Microbiol 57, 527–531.[CrossRef] [Google Scholar]
  21. Reichenbach, H.(1992). The order Cytophagales. In The Prokaryotes. A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications, 2nd edn, pp. 3631–3675. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  22. Saha, P. & Chakrabarti, T.(2006).Flavobacterium indicum sp. nov., isolated from warm spring water in Assam, India. Int J Syst Evol Microbiol 56, 2617–2621.[CrossRef] [Google Scholar]
  23. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  24. Sharma, P., Raina, V., Kumari, R., Malhotra, S., Dogra, C., Kumari, H., Kohler, H. P., Buser, H. R., Holliger, C. & Lal, R.(2006). The haloalkane dehalogenase LinB is responsible for β- and δ-hexachlorocyclohexane transformation in Sphingobium indicum B90A. Appl Environ Microbiol 72, 5720–5727.[CrossRef] [Google Scholar]
  25. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  26. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  27. Van de Peer, Y. & De Wachter, R.(1994).treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10, 569–570. [Google Scholar]
  28. Van Trappen, S., Mergaert, J. & Swings, J.(2003).Flavobacterium gelidilacus sp. nov., isolated from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 53, 1241–1245.[CrossRef] [Google Scholar]
  29. Van Trappen, S., Vandecandelaere, I., Mergaert, J. & Swings, J.(2004).Flavobacterium degerlachei sp. nov., Flavobacterium frigoris sp. nov. and Flavobacterium micromati sp. nov., novel psychrophilic bacteria isolated from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 54, 85–92.[CrossRef] [Google Scholar]
  30. Vanbroekhoven, K., Ryngaert, A., Bastiaens, L., Wattiau, P., Vancanneyt, M., Swings, J., Mot, R. D. & Springael, D.(2004). Streptomycin as a selective agent to facilitate recovery and isolation of introduced and indigenous sphingomonas from environmental samples. Environ Microbiol 6, 1123–1136.[CrossRef] [Google Scholar]
  31. Wang, Z. W., Liu, Y. H., Dai, X., Wang, B. J., Jiang, C. Y. & Liu, S. J.(2006).Flavobacterium saliperosum sp. nov., isolated from freshwater lake sediment. Int J Syst Evol Microbiol 56, 439–442.[CrossRef] [Google Scholar]
  32. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  33. Weon, H. Y., Song, M. H., Son, J. A., Kim, B. Y., Kwon, S. W., Go, S. J. & Stackebrandt, E.(2007).Flavobacterium terrae sp. nov. and Flavobacterium cucumis sp. nov., isolated from greenhouse soil. Int J Syst Evol Microbiol 57, 1594–1598.[CrossRef] [Google Scholar]
  34. Woese, C. R., Mandelco, L., Yang, D., Gherna, R. & Madigan, M. T.(1990). The case for relationship of the Flavobacteria and their relatives to the green sulfur bacteria. Syst Appl Microbiol 13, 258–262.[CrossRef] [Google Scholar]
  35. Yoon, J.-H., Kang, S.-J. & Oh, T.-K.(2006).Flavobacterium soli sp. nov., isolated from soil. Int J Syst Evol Microbiol 56, 997–1000.[CrossRef] [Google Scholar]

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vol. , part 7, pp. 1665 - 1669

A phylogenetic tree obtained by using the EzTaxon server. [ PDF] 22 KB

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