A novel actinomycete strain, designated HSW-1T, was isolated from seawater collected from Hwasun Beach on the coast of Jeju Island, Republic of Korea. The cells were aerobic, Gram-positive, oxidase-negative, catalase-positive, non-motile rods. The colonies were circular, smooth, convex and yellow in colour. The cell-wall peptidoglycan of this organism contained ll-diaminopimelic acid as the diagnostic diamino acid. The polar lipids included phosphatidylinositol, diphosphatidylglycerol, phosphatidylglycerol and three unknown phospholipids. The predominant menaquinone was MK-9(H4). The major fatty acids were C18 : 1ω9c, C16 : 0, C16 : 0 2-OH and 10-methyl C18 : 0. The DNA G+C content was 74.0 mol%. A neighbour-joining tree based on 16S rRNA gene sequences showed that this organism falls within the radiation of the genus Aeromicrobium. The closest phylogenetic neighbours were the type strains of Aeromicrobium erythreum (98.2 % 16S rRNA gene sequence similarity), Aeromicrobium alkaliterrae (97.9 %), Aeromicrobium marinum (97.3 %) and Aeromicrobium fastidiosum (97.0 %). The DNA–DNA relatedness values between the novel isolate and its closest relative, A. erythreum DSM 8599T, ranged between 32 and 36 %. On the basis of the phenotypic and DNA–DNA hybridization data, strain HSW-1T represents a novel species of the genus Aeromicrobium, for which the name Aeromicrobium ponti sp. nov. is proposed. The type strain is HSW-1T (=DSM 19178T=KACC 20565T).
Bruns, A., Philipp, H., Cypionka, H. & Brinkhoff, T.(2003).Aeromicrobium marinum sp. nov., an abundant pelagic bacterium isolated from the German Wadden Sea. Int J Syst Evol Microbiol53, 1917–1923.[CrossRef][Google Scholar]
Cashion, P., Holder-Franklin, M. A., McCully, J. & Franklin, M.(1977). A rapid method for base ratio determination of bacterial DNA. Anal Biochem81, 461–466.[CrossRef][Google Scholar]
Collins, M. D.(1985). Analysis of isoprenoid quinones. Methods Microbiol18, 329–366.
[Google Scholar]
Cui, Y. S., Im, W. T., Yin, C. R., Lee, J. S., Lee, K. C. & Lee, S. T.(2007).Aeromicrobium panaciterrae sp. nov., isolated from soil of a ginseng field in South Korea. Int J Syst Evol Microbiol57, 687–691.[CrossRef][Google Scholar]
De Ley, J., Cattoir, H. & Reynaerts, A.(1970). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem12, 133–142.[CrossRef][Google Scholar]
Felsenstein, J.(1981). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol17, 368–376.[CrossRef][Google Scholar]
Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution39, 783–791.[CrossRef][Google Scholar]
Fitch, W. M.(1971). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool20, 406–416.[CrossRef][Google Scholar]
Hopwood, D. A., Bibb, M. J., Chater, K. F., Kieser, T., Bruton, C. J., Kieser, H. M., Lydiate, D. J., Smith, C. P., Ward, J. M. & Schrempf, H.(1985).Genetic Manipulation of Streptomyces: a Laboratory Manual. Norwich: John Innes Foundation.
Huß, V. A. R., Festl, H. & Schleifer, K. H.(1983). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol4, 184–192.[CrossRef][Google Scholar]
Jukes, T. H. & Cantor, C. R.(1969). Evolution of protein molecules. In Mammalian Protein Metabolism, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
Kroppenstedt, R. M.(1985). Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics, pp. 173–199. Edited by M. Goodfellow & D. E. Minnikin. London: Academic Press.
Lane, D. J.(1991). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–144. Edited by E. Stackebrandt & M. Goodfellow. London: Wiley.
Lee, S. D. & Kim, S. J.(2007).Aeromicrobium tamlense sp. nov., isolated from dried seaweed. Int J Syst Evol Microbiol57, 337–341.[CrossRef][Google Scholar]
Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol39, 159–167.[CrossRef][Google Scholar]
Miller, E. S., Woese, C. R. & Brenner, S.(1991). Description of the erythromycin-producing bacterium Arthrobacter sp. strain NRRL B-3381 as Aeromicrobium erythreum gen. nov., sp. nov. Int J Syst Bacteriol41, 363–368.[CrossRef][Google Scholar]
Minnikin, D. E., Alshamaony, L. & Goodfellow, M.(1975). Differentiation of Mycobacterium, Nocardia, and related taxa by thin layer chromatographic analysis of whole-organism methanolysates. J Gen Microbiol88, 200–204.[CrossRef][Google Scholar]
Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol4, 406–425.
[Google Scholar]
Shirling, E. B. & Gottlieb, D.(1966). Methods for characterization of Streptomyces species. Int J Syst Bacteriol16, 313–340.[CrossRef][Google Scholar]
Staneck, J. L. & Roberts, G. D.(1974). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol28, 226–231.
[Google Scholar]
Tamura, T. & Yokota, A.(1994). Transfer of Nocardioides fastidiosa Collins and Stackebrandt 1989 to the genus Aeromicrobium as Aeromicrobium fastidiosum comb. nov. Int J Syst Bacteriol44, 608–611.[CrossRef][Google Scholar]
Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res25, 4876–4882.[CrossRef][Google Scholar]
Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol37, 463–464.[CrossRef][Google Scholar]
Yoon, J.-H., Lee, C.-H. & Oh, T. K.(2005).Aeromicrobium alkaliterrae sp. nov., isolated from an alkali soil, and emended description of the genus Aeromicrobium. Int J Syst Evol Microbiol55, 2171–2175.[CrossRef][Google Scholar]