A novel actinomycete strain, designated HSW-1, was isolated from seawater collected from Hwasun Beach on the coast of Jeju Island, Republic of Korea. The cells were aerobic, Gram-positive, oxidase-negative, catalase-positive, non-motile rods. The colonies were circular, smooth, convex and yellow in colour. The cell-wall peptidoglycan of this organism contained -diaminopimelic acid as the diagnostic diamino acid. The polar lipids included phosphatidylinositol, diphosphatidylglycerol, phosphatidylglycerol and three unknown phospholipids. The predominant menaquinone was MK-9(H). The major fatty acids were C 9, C, C 2-OH and 10-methyl C. The DNA G+C content was 74.0 mol%. A neighbour-joining tree based on 16S rRNA gene sequences showed that this organism falls within the radiation of the genus . The closest phylogenetic neighbours were the type strains of (98.2 % 16S rRNA gene sequence similarity), (97.9 %), (97.3 %) and (97.0 %). The DNA–DNA relatedness values between the novel isolate and its closest relative, DSM 8599, ranged between 32 and 36 %. On the basis of the phenotypic and DNA–DNA hybridization data, strain HSW-1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HSW-1 (=DSM 19178=KACC 20565).


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Cellular fatty acid profiles (%) of strain HSW-1 and the type strains of the species of the genus [ PDF] 44 KB


Transmission electron micrograph of a cell of strain HSW-1 grown on TSA for 24 h at 30 °C. [ PDF] 28 KB

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