sp. nov., isolated from soil on Bigeum Island, Korea Free

Abstract

A Gram-positive, motile, mesophilic bacterium that formed yellowish-white, circular, convex, glistening colonies, strain MSL-27, was isolated from a soil sample from Bigeum Island, Korea, and its taxonomic position was investigated by a polyphasic approach. Cells of strain MSL-27 are non-spore-forming, irregular rods, usually fragmenting into shorter rods or coccoid elements. The growth temperature and pH ranges are 20–37 °C and pH 6.0–12.0, with optimum growth at 28 °C and pH 8.0. MK-11 is the major menaquinone and -2,4-diaminobutyric acid is found in the cell-wall peptidoglycan. The major fatty acids are i-C (39 %), ai-C (36 %), ai-C (18 %) and i-C (2 %). The G+C content is 69.0 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain MSL-27 should be placed in the genus . Levels of similarity between the 16S rRNA gene sequence of strain MSL-27 and those of the type strains of the other members of the genus ranged from 95.8 to 96.7 %. Based on phenotypic characteristics, biochemical properties and chemotaxonomic features, strain MSL-27 (=KCTC 19268 =DSM 19322) represents the type strain of a novel species of , for which the name sp. nov. is proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65572-0
2008-08-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/8/1935.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65572-0&mimeType=html&fmt=ahah

References

  1. Chun, J., Lee, J.-H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y. W.(2007). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequence. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef] [Google Scholar]
  2. Cui, X. L., Mao, P. H., Zeng, M., Li, W. J., Zhang, L. P., Xu, L. H. & Jiang, C. L.(2001).Streptimonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae. Int J Syst Evol Microbiol 51, 357–363. [Google Scholar]
  3. Davis, M. J., Gillaspie, A. G., Jr, Vidaver, A. K. & Harris, R. W.(1984).Clavibacter: a new genus containing some phytopathogenic coryneform bacteria, including Clavibacter xyli subsp. xyli sp. nov., subsp. nov. and Clavibacter xyli subsp. cynodontis subsp. nov., pathogens that cause ratoon stunting disease of sugarcane and Bermudagrass stunting disease. Int J Syst Bacteriol 34, 107–117.[CrossRef] [Google Scholar]
  4. Evtushenko, L. I., Dorofeeva, L. V., Subbotin, S. A., Cole, J. R. & Tiedje, J. M.(2000).Leifsonia poae gen. nov., sp. nov., isolated from nematode galls on Poa annua, and reclassification of ‘Corynebacterium aquaticum’ Leifson 1962 as Leifsonia aquatica (ex Leifson 1962 ) gen. nov., nom. rev., comb. nov. and Clavibacter xyli Davis et al. 1984 with two subspecies as Leifsonia xyli ( Davis et al. 1984 ) gen. nov., comb. nov. Int J Syst Evol Microbiol 50, 371–380.[CrossRef] [Google Scholar]
  5. Felsenstein, J.(1981). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef] [Google Scholar]
  6. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  7. Felsenstein, J.(1993).phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  8. Fitch, W. M. & Margoliash, E.(1967). Construction of phylogenetic trees: a method based on mutation distances as estimated from cytochrome c sequences is of general applicability. Science 155, 279–284.[CrossRef] [Google Scholar]
  9. Kämpfer, P.(1991). Application of miniaturized physiological tests in numerical classification and identification of some bacilli. J Gen Appl Microbiol 37, 225–247.[CrossRef] [Google Scholar]
  10. Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  11. Kimura, M.(1983).The Neutral Theory of Molecular Evolution. Cambridge: Cambridge University Press.
  12. Kluge, A. G. & Farris, F. S.(1969). Quantitative phyletics and the evolution of anurans. Syst Zool 18, 1–32.[CrossRef] [Google Scholar]
  13. Kroppenstedt, R. M.(1982). Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 5, 2359–2367.[CrossRef] [Google Scholar]
  14. Kumar, S., Tamura, K., Jakobsen, I.-B. & Nei, M.(2001).mega 2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef] [Google Scholar]
  15. Leifson, E.(1962). The bacterial flora of distilled and stored water. III. New species of the genera Corynebacterium, Flavobacterium, Spirillum and Pseudomonas. Int Bull Bacteriol Nomencl Taxon 12, 161–170. [Google Scholar]
  16. Marmur, J.(1961). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef] [Google Scholar]
  17. Marmur, J. & Doty, P.(1962). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef] [Google Scholar]
  18. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H.(1984). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef] [Google Scholar]
  19. Qiu, F., Huang, Y., Sun, L., Zhang, X., Liu, Z. & Song, W.(2007).Leifsonia ginsengi sp. nov., isolated from ginseng root. Int J Syst Evol Microbiol 57, 405–408.[CrossRef] [Google Scholar]
  20. Reddy, G. S. N., Prakash, J. S. S., Srinivas, R., Matsumoto, G. I. & Shivaji, S.(2003).Leifsonia rubra sp. nov. and Leifsonia aurea sp. nov., psychrophiles from a pond in Antarctica. Int J Syst Evol Microbiol 53, 977–984.[CrossRef] [Google Scholar]
  21. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  22. Sasser, M.(1990). Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20, 16 [Google Scholar]
  23. Schleifer, K. H. & Kandler, O.(1972). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477. [Google Scholar]
  24. Staneck, J. L. & Roberts, G. D.(1974). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226–231. [Google Scholar]
  25. Suzuki, K., Suzuki, M., Sasaki, J., Park, Y. H. & Komagata, K.(1999).Leifsonia gen. nov., a genus for 2,4-diaminobutyric acid containing actinomycetes to accommodate “Corynebacterium aquaticumLeifson 1962 and Clavibacter xyli subsp. cynodontis Davis et al. 1984 . J Gen Appl Microbiol 45, 253–262.[CrossRef] [Google Scholar]
  26. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4888.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65572-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65572-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed