sp. nov., isolated from rhizosphere soil of (Champ. ex Benth.) Swingle Free

Abstract

The taxonomic status of a pale-yellow-coloured bacterial isolate from rhizosphere soil of (Champ. ex Benth.) Swingle was characterized using a polyphasic taxonomic approach. Comparative analysis of the 16S rRNA gene sequence showed that the isolate constituted a distinct branch within the genus . The generic assignment was confirmed by chemotaxonomic data, which revealed the presence of a fatty acid profile that was characteristic for the genus , consisting of straight-chain saturated and unsaturated as well as 2-OH fatty acids and the lack of 3-OH fatty acids, ubiquinone with ten isoprene units (Q-10) as the predominant respiratory quinone, and a polar lipid pattern that consisted of the predominant compounds phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine, diphosphatidylglycerol, sphingoglycolipid and an unknown glycolipid. Spermidine was the major polyamine component. The genotypic and phenotypic data (physiology and fatty acid and polar lipid profiles) showed that the isolate merits classification as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CC-FH12-1 (=CCM 7941=BCRC 17770).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65564-0
2008-08-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/8/1801.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65564-0&mimeType=html&fmt=ahah

References

  1. Altenburger, P., Kämpfer, P., Makristathis, A., Lubitz, W. & Busse, H.-J.(1996). Classification of bacteria isolated from a medieval wall painting. J Biotechnol 47, 39–52.[CrossRef] [Google Scholar]
  2. Busse, H.-J. & Auling, G.(1988). Polyamine pattern as a chemotaxonomic marker within the Proteobacteria. Syst Appl Microbiol 11, 1–8.[CrossRef] [Google Scholar]
  3. Busse, H.-J., Bunka, S., Hensel, A. & Lubitz, W.(1997). Discrimination of members of the family Pasteurellaceae based on polyamine patterns. Int J Syst Bacteriol 47, 698–708.[CrossRef] [Google Scholar]
  4. Busse, H.-J., Kämpfer, P. & Denner, E. B. M.(1999). Chemotaxonomic characterisation of Sphingomonas. J Ind Microbiol Biotechnol 23, 242–251.[CrossRef] [Google Scholar]
  5. Felsenstein, J.(1981). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef] [Google Scholar]
  6. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  7. Fitch, W. M.(1971). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef] [Google Scholar]
  8. Jukes, T. H. & Cantor, C. R.(1969). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  9. Kämpfer, P. & Kroppenstedt, R. M.(1996). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef] [Google Scholar]
  10. Kim, S.-J., Chun, J., Bae, K. S. & Kim, Y.-C.(2000). Polyphasic assignment of an aromatic degrading Pseudomonas sp., strain DJ77, in the genus Sphingomonas as Sphingomonas chungbukensis sp. nov. Int J Syst Evol Microbiol 50, 1641–1647.[CrossRef] [Google Scholar]
  11. Ludwig, W., Strunk, O., Westram, R., Richter, L., Meier, H., Yadhukumar, Buchner, A., Lai, T., Steppi, S. & other authors(2004).arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef] [Google Scholar]
  12. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  13. Pal, R., Bala, S., Dadhwal, M., Kumar, M., Dhingra, G., Prakash, O., Prabagaran, S. R., Shivaji, S., Cullum, J. & other authors(2005). Hexachlorocyclohexane-degrading bacterial strains Sphingomonas paucimobilis B90A, UT26 and Sp+, having similar lin genes, represent three distinct species, Sphingobium indicum sp. nov., Sphingobium japonicum sp. nov. and Sphingobium francense sp. nov., and reclassification of [Sphingomonas] chungbukensis as Sphingobium chungbukense comb. nov. Int J Syst Evol Microbiol 55, 1965–1972.[CrossRef] [Google Scholar]
  14. Prakash, O. & Lal, R.(2006). Description of Sphingobium fuliginis sp. nov., a phenanthrene-degrading bacterium from a fly ash dumping site, and reclassification of Sphingomonas cloacae as Sphingobium cloacae comb. nov. Int J Syst Evol Microbiol 56, 2147–2152.[CrossRef] [Google Scholar]
  15. Rainey, F. A., Ward-Rainey, N., Kroppenstedt, R. M. & Stackebrandt, E.(1996). The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsiaceae fam. nov. Int J Syst Bacteriol 46, 1088–1092.[CrossRef] [Google Scholar]
  16. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  17. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  18. Stolz, A., Schmidt-Maag, C., Denner, E. B. M., Busse, H.-J., Egli, T. & Kämpfer, P.(2000). Description of Sphingomonas xenophaga sp. nov. for strains BN6T and N,N which degrade xenobiotic aromatic compounds. Int J Syst Evol Microbiol 50, 35–41.[CrossRef] [Google Scholar]
  19. Stolz, A., Busse, H.-J. & Kämpfer, P.(2007).Pseudomonas knackmussii sp. nov. Int J Syst Evol Microbiol 57, 572–576.[CrossRef] [Google Scholar]
  20. Takeuchi, M., Hamana, K. & Hiraishi, A.(2001). Proposal of the genus Sphingomonas sensu stricto and three new genera, Sphingobium, Novosphingobium and Sphingopyxis, on the basis of phylogenetic and chemotaxonomic analyses. Int J Syst Evol Microbiol 51, 1405–1417. [Google Scholar]
  21. Tindall, B. J.(1990a). Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 66, 199–202.[CrossRef] [Google Scholar]
  22. Tindall, B. J.(1990b). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13, 128–130.[CrossRef] [Google Scholar]
  23. Ushiba, Y., Takahara, Y. & Ohta, H.(2003).Sphingobium amiense sp. nov., a novel nonylphenol-degrading bacterium isolated from a river sediment. Int J Syst Evol Microbiol 53, 2045–2048.[CrossRef] [Google Scholar]
  24. Wittich, R.-M., Busse, H.-J., Kämpfer, P., Tiirola, M., Wieser, M., Macedo, A. J. & Abraham, W.-R.(2007).Sphingobium aromaticiconvertens sp. nov., a xenobiotic-compound-degrading bacterium from polluted river sediment. Int J Syst Evol Microbiol 57, 306–310.[CrossRef] [Google Scholar]
  25. Yabuuchi, E., Yano, I., Oyaizu, H., Hashimoto, Y., Ezaki, T. & Yamamoto, H.(1990). Proposals of Sphingomonas paucimobilis gen. nov. and comb. nov., Sphingomonas parapaucimobilis sp. nov., Sphingomonas yanoikuyae sp. nov., Sphingomonas adhaesiva sp. nov., Sphingomonas capsulata comb. nov., and two genospecies of the genus Sphingomonas. Microbiol Immunol 34, 99–119.[CrossRef] [Google Scholar]
  26. Young, C.-C., Ho, M.-J., Arun, A. B., Chen, W.-M., Lai, W.-A., Shen, F.-T., Rekha, P. D. & Yassin, A. F.(2007).Sphingobium olei sp. nov., isolated from oil-contaminated soil. Int J Syst Evol Microbiol 57, 2613–2617.[CrossRef] [Google Scholar]
  27. Ziemke, F., Höfle, M. G., Lalucat, J. & Rosselló-Mora, R.(1998). Reclassification of Shewanella putrefaciens Owen's genomic group II as Shewanella baltica sp. nov. Int J Syst Bacteriol 48, 179–186.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65564-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65564-0
Loading

Data & Media loading...

Supplements

Polar lipid profile of strain CC-FH12-1 ( sp. nov.). [PDF](218 KB)

PDF

Cellular fatty acid contents of strain CC-FH12-1 ( sp. nov.) and recognized species. [PDF](30 KB)

PDF

Most cited Most Cited RSS feed