1887

Abstract

A Gram-positive, cream-coloured, non-motile, rod-shaped actinomycete, designated DLS-10, was isolated from a rock collected on the peak of Darangshi Oreum (a small mountain 300 m above sea level) in Jeju, Republic of Korea. The temperature and pH ranges for growth were 4–37 °C and pH 5.1–9.1. The organism grew at NaCl concentrations up to 3 % and had the following chemotaxonomic characteristics: -diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan, the polar lipids included diphosphatidylglycerol, phosphatidylmethylethanolamine and two unknown phospholipids, the major menaquinone was MK-8(H) and the major fatty acids were anteiso-C, C, iso-C and anteiso-C. The DNA G+C content was 74.3 mol%. Phylogenetic analyses based on 16S rRNA gene sequences revealed that the organism was related to members of the family in the suborder . The most closely related described organisms were the type strains of (97.1 % sequence similarity) and (95.2 %). On the basis of the morphological, cultural, physiological, chemotaxonomic and phylogenetic evidence presented here, the organism represents a novel genus and species of the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is DLS-10 (=KCTC 19285 =DSM 19367).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65558-0
2008-04-01
2021-10-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/4/906.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65558-0&mimeType=html&fmt=ahah

References

  1. Felsenstein, J.(1981). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef] [Google Scholar]
  2. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  3. Felsenstein, J.(1993).phylip (phylogeny inference package), version 3.5c. Department of Genome Sciences, University of Washington, Seattle, USA.
  4. Fitch, W. M.(1971). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef] [Google Scholar]
  5. Jukes, T. H. & Cantor, C. R.(1969). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  6. Kroppenstedt, R. M.(1985). Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics, pp. 173–199. Edited by M. Goodfellow & D. E. Minnikin. London: Academic Press.
  7. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  8. Minnikin, D. E., Alshamaony, L. & Goodfellow, M.(1977). Differentiation of Mycobacterium, Nocardia, and related taxa by thin layer chromatographic analysis of whole-cell methanolysates. J Gen Microbiol 88, 200–204. [Google Scholar]
  9. Saddler, G. S., Tavecchia, P., Lociuro, S., Zanol, M., Colombo, L. & Selva, E.(1991). Analysis of madurose and other actinomycete whole cell sugars by gas chromatography. J Microbiol Methods 14, 185–191.[CrossRef] [Google Scholar]
  10. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  11. Seo, J. P. & Lee, S. D.(2006).Nocardia harenae sp. nov., an actinomycete isolated from beach sand. Int J Syst Evol Microbiol 56, 2203–2207.[CrossRef] [Google Scholar]
  12. Stackebrandt, E., Rainey, F. A. & Ward-Rainey, N. L.(1997). Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47, 479–491.[CrossRef] [Google Scholar]
  13. Staneck, J. L. & Roberts, G. D.(1974). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226–231. [Google Scholar]
  14. Tao, T.-S., Yue, Y.-Y., Chen, W.-X. & Chen, W.-F.(2004). Proposal of Nakamurella gen. nov. as a substitute for the bacterial genus Microsphaera Yoshimi et al. 1996 and Nakamurellaceae fam. nov. as a substitute for the illegitimate bacterial family Microsphaeraceae Rainey et al. 1997. Int J Syst Evol Microbiol 54, 999–1000.[CrossRef] [Google Scholar]
  15. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  16. Yoon, J.-H., Kang, S.-J., Jung, S.-Y. & Oh, T.-K.(2007).Humicoccus flavidus gen. nov., sp. nov., isolated from soil. Int J Syst Evol Microbiol 57, 56–59.[CrossRef] [Google Scholar]
  17. Yoshimi, Y., Hiraishi, A. & Nakamura, K.(1996). Isolation and characterization of Microsphaera multipartita gen. nov., sp. nov., a polysaccharide-accumulating Gram-positive bacterium from activated sludge. Int J Syst Bacteriol 46, 519–525.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65558-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65558-0
Loading

Data & Media loading...

Supplements

Transmission electron micrograph of cells of strain DLS-10 grown on TSA for 3 days at 30 °C. Bar, 0.5 µm.

IMAGE

Cellular fatty acid compositions of strain DLS-10 and related members of the family . [PDF](33 KB)

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error