A Gram-positive, cream-coloured, non-motile, rod-shaped actinomycete, designated DLS-10, was isolated from a rock collected on the peak of Darangshi Oreum (a small mountain 300 m above sea level) in Jeju, Republic of Korea. The temperature and pH ranges for growth were 4–37 °C and pH 5.1–9.1. The organism grew at NaCl concentrations up to 3 % and had the following chemotaxonomic characteristics: -diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan, the polar lipids included diphosphatidylglycerol, phosphatidylmethylethanolamine and two unknown phospholipids, the major menaquinone was MK-8(H) and the major fatty acids were anteiso-C, C, iso-C and anteiso-C. The DNA G+C content was 74.3 mol%. Phylogenetic analyses based on 16S rRNA gene sequences revealed that the organism was related to members of the family in the suborder . The most closely related described organisms were the type strains of (97.1 % sequence similarity) and (95.2 %). On the basis of the morphological, cultural, physiological, chemotaxonomic and phylogenetic evidence presented here, the organism represents a novel genus and species of the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is DLS-10 (=KCTC 19285 =DSM 19367).


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Transmission electron micrograph of cells of strain DLS-10 grown on TSA for 3 days at 30 °C. Bar, 0.5 µm.


Cellular fatty acid compositions of strain DLS-10 and related members of the family . [PDF](33 KB)

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