1887

Abstract

A novel bacterium, MTT-39, was isolated from a sample of marine sediment collected at Tottori on the coast of the Sea of Japan. Cells were Gram-negative, rod-shaped and non-motile. The bacterium formed yellowish brown colonies on marine agar 2216. Although the 16S rRNA gene sequence of strain MTT-39 classified this strain as a member of the family , the maximum sequence similarity obtained was only 91.5 % (with OT-1). In the maximum-likelihood tree based on 16S rRNA gene sequences, the novel bacterium clustered with the type strains of , , and . The novel strain exhibited the following characteristics: the predominant fatty acids in cells grown on artificial seawater-based tryptic soya agar were iso-C, iso-C and iso-C 3-OH, the major respiratory quinone was MK-6 and the DNA G+C content was 35 mol%. On the basis of its distinct phenotypic traits and the phylogenetic distance between this marine isolate and other recognized taxa, strain MTT-39 represents a novel genus and species of the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is MTT-39 (=NBRC 102624=KCTC 22214=CGMCC 1.7058).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65554-0
2008-07-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/7/1670.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65554-0&mimeType=html&fmt=ahah

References

  1. Alonso, C., Warnecke, F., Amann, R. & Pernthaler, J.(2007). High local and global diversity of Flavobacteria in marine plankton. Environ Microbiol 9, 1253–1266.[CrossRef] [Google Scholar]
  2. Bernardet, J.-F. & Nakagawa, Y.(2006). An introduction to the family Flavobacteriaceae. In The Prokaryotes: a Handbook on the Biology of Bacteria, 3rd edn, vol. 7, pp. 455–480. Edited by M. Dworkin, S. Falkow, E. Rosenberg, K.-H. Schleifer & E. Stackebrandt. New York: Springer.
  3. Bernardet, J.-F., Nakagawa, Y. & Holmes, B.(2002). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52, 1049–1070.[CrossRef] [Google Scholar]
  4. Bowman, J. P.(2006). The marine clade of the family Flavobacteriaceae: the genera Aequorivita, Arenibacter, Cellulophaga, Croceibacter, Formosa, Gelidibacter, Gillisia, Maribacter, Mesonia, Muricauda, Polaribacter, Psychroflexus, Psychroserpens, Robiginitalea, Salegentibacter, Tenacibaculum, Ulvibacter, Vitellibacter and Zobellia. In The Prokaryotes: a Handbook on the Biology of Bacteria, 3rd edn, vol. 7, pp. 677–694. Edited by M. Dworkin, S. Falkow, E. Rosenberg, K.-H. Schleifer & E. Stackebrandt. New York: Springer.
  5. Buck, J. D.(1982). Nonstaining (KOH) method for determination of Gram reactions of marine bacteria. Appl Environ Microbiol 44, 992–993. [Google Scholar]
  6. Choi, D. H. & Cho, B. C.(2006).Lutibacter litoralis gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from tidal flat sediment. Int J Syst Evol Microbiol 56, 771–776.[CrossRef] [Google Scholar]
  7. Choi, D. H., Kim, Y.-G., Hwang, C. Y., Yi, H., Chun, J. & Cho, B. C.(2006).Tenacibaculum litoreum sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 56, 635–640.[CrossRef] [Google Scholar]
  8. Cottrell, M. T. & Kirchman, D. L.(2000). Natural assemblages of marine proteobacteria and members of the Cytophaga-Flavobacter cluster consuming low- and high-molecular-weight dissolved organic matter. Appl Environ Microbiol 66, 1692–1697.[CrossRef] [Google Scholar]
  9. Cowan, S. T. & Steel, K. J.(1993).Manual for the Identification of Medical Bacteria, 3rd edn. London: Cambridge University Press.
  10. Fautz, E. & Reichenbach, H.(1980). A simple test for flexirubin-type pigments. FEMS Microbiol Lett 8, 87–91.[CrossRef] [Google Scholar]
  11. Glöckner, F. O., Fuchs, B. M. & Amann, R.(1999). Bacterioplankton compositions of lakes and oceans: a first comparison based on fluorescence in situ hybridization. Appl Environ Microbiol 65, 3721–3726. [Google Scholar]
  12. Gosink, J. J., Woese, C. R. & Staley, J. T.(1998).Polaribacter gen. nov., with three new species, P. irgensii sp. nov., P. franzmannii sp. nov. and P. filamentus sp. nov., gas vacuolate polar marine bacteria of the Cytophaga–Flavobacterium–Bacteroides group and reclassification of ‘Flectobacillus glomeratu’ as Polaribacter glomeratus comb. nov. Int J Syst Evol Microbiol 48, 223–235. [Google Scholar]
  13. Jung, S.-Y., Oh, T.-K. & Yoon, J.-H.(2006).Tenacibaculum aestuarii sp. nov., isolated from tidal flat sediment in Korea. Int J Syst Evol Microbiol 56, 1577–1581.[CrossRef] [Google Scholar]
  14. Khan, S. T., Nakagawa, Y. & Harayama, S.(2007).Sediminibacter furfurosus gen. nov., sp. nov. and Gilvibacter sediminis gen. nov., sp. nov., novel members of the family Flavobacteriaceae. Int J Syst Evol Microbiol 57, 265–269.[CrossRef] [Google Scholar]
  15. Lewin, R. A. & Lounsbery, D. C.(1969). Isolation, cultivation and characterization of flexibacteria. J Gen Microbiol 58, 145–170.[CrossRef] [Google Scholar]
  16. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  17. Minamisawa, K.(1990). Division of rhizobitoxine-producing and hydrogen-uptake positive strains of Bradyrhizobium japonicum by nifDKE sequence divergence. Plant Cell Physiol 31, 81–89. [Google Scholar]
  18. Nakagawa, Y. & Yamasato, K.(1993). Phylogenetic diversity of the genus Cytophaga revealed by 16S rRNA sequencing and menaquinone analysis. J Gen Microbiol 139, 1155–1161.[CrossRef] [Google Scholar]
  19. Nedashkovskaya, O. I., Kim, S. B., Lysenko, A. M., Kalinovskaya, N. I., Mikhailov, V. V., Kim, S. I. & Bae, S. K.(2005).Polaribacter butkevichii sp. nov., a novel marine mesophilic bacterium of the family Flavobacteriaceae. Curr Microbiol 51, 408–412.[CrossRef] [Google Scholar]
  20. Needleman, S. B. & Wunsch, C. D.(1970). A general method applicable to the search for similarities in the amino acid sequence of two proteins. J Mol Biol 48, 443–453.[CrossRef] [Google Scholar]
  21. Perry, L. B.(1973). Gliding motility in some non-spreading flexibacteria. J Appl Bacteriol 36, 227–232.[CrossRef] [Google Scholar]
  22. Rüger, H.-J. & Krambeck, H.-J.(1994). Evaluation of the BIOLOG substrate metabolism system for classification of marine bacteria. Syst Appl Microbiol 17, 281–288.[CrossRef] [Google Scholar]
  23. Sheu, S.-Y., Lin, K.-Y., Chou, J.-S., Chang, P.-S., Arun, A. B., Young, C.-C. & Chen, W.-M.(2007).Tenacibaculum litopenaei sp. nov., isolated from a shrimp mariculture pond. Int J Syst Evol Microbiol 57, 1148–1153.[CrossRef] [Google Scholar]
  24. Smibert, R. M. & Krieg, N. R.(1981). General characterization. In Manual of Methods for General Microbiology, pp. 409–443. Edited by P. Gerhardt, R. G. E. Murray, R. N. Costilow, E. W. Nester, W. A. Wood, N. R. Krieg & G. B. Phillips. Washington, DC: American Society for Microbiology.
  25. Sohn, J. H., Lee, J.-H., Yi, H., Chun, J., Bae, K. S., Ahn, T.-Y. & Kim, S. J.(2004).Kordia algicida gen. nov., sp. nov., an algicidal bacterium from red tide. Int J Syst Evol Microbiol 54, 675–680.[CrossRef] [Google Scholar]
  26. Suzuki, M., Nakagawa, Y., Harayama, S. & Yamamoto, S.(2001). Phylogenetic analysis and taxonomic study of marine cytophaga-like bacteria: proposal for Tenacibaculum gen. nov., with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov. Int J Syst Evol Microbiol 51, 1639–1652.[CrossRef] [Google Scholar]
  27. Yoon, J.-H., Kang, S.-J. & Oh, T.-K.(2005).Tenacibaculum lutimaris sp. nov., isolated from a tidal flat in the Yellow Sea, Korea. Int J Syst Evol Microbiol 55, 793–798.[CrossRef] [Google Scholar]
  28. Yoon, J.-H., Kang, S.-J. & Oh, T.-K.(2006).Tenacibaculum dokdonensis sp. nov., isolated from seawater. Int J Syst Evol Microbiol 56, 1251–1255.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65554-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65554-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error