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Volume 58, Issue 12

gen. nov., sp. nov., a Gram-positive, strictly anaerobic bacterium isolated from the faeces of thoroughbred horses Free

Abstract

Four bacterial strains, designated ST18, HM244, HM250 and DI49, were isolated from the fresh faeces of four thoroughbred horses in Japan. Cells were Gram-positive, strictly anaerobic, catalase-negative, non-spore-forming, non-motile rods that occurred in chains. They were placed in the same subcluster based on 16S rRNA gene sequence analysis, phenotypic characteristics and levels of DNA–DNA relatedness. Their DNA G+C content ranged from 36 to 38 mol%. , and belong to cluster XVII of the subphylum. Strain ST18 was most closely related to ATCC 25536 in the phylogenetic tree, but these strains shared only 89.9 % (1336/1486 bp) 16S rRNA gene sequence similarity. , and are homofermentative bacteria, whereas ST18 produced CO from glucose. Whereas the cell-wall peptidoglycan type of and was -Lys–-Ala, that of and the subgroup represented by ST18 was A1 (-Ala–-Glu–-diaminopimelic acid). On the basis of 16S rRNA gene sequence divergence of more than 10 % from as well as phenotypic characteristics, strains ST18, HM244, HM250 and DI49 are considered to represent a novel species of a new genus belonging to the subphylum cluster XVII, for which the name gen. nov., sp. nov. is proposed. The type strain of is ST18 (=JCM 14210 =DSM 18934).

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Keyword(s): m-Dpm, meso-diaminopimelic acid
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2008-12-01
2023-09-21
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Sharpea azabuensis gen. nov., sp. nov., a Gram-positive, strictly anaerobic bacterium isolated from the faeces of thoroughbred horses
Int J Syst Evol Microbiol 58, 2682 (2008); https://doi.org/10.1099/ijs.0.65543-0
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vol. , part 12, pp. 2682 - 2686

Details of isolation of strains ST18 , HM244, HM250 and DI49

DNA–DNA relatedness between strain ST18 and related type strains

Phylogenetic relationships of strains ST18 , HM244, HM250 and DI49 with species in clusters XV–XIX of the subphylum based on 16S rRNA gene sequences. The trees were constructed using the maximum-parsimony method (S1) and the maximum-likelihood method (S2).

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