Four bacterial strains, designated ST18, HM244, HM250 and DI49, were isolated from the fresh faeces of four thoroughbred horses in Japan. Cells were Gram-positive, strictly anaerobic, catalase-negative, non-spore-forming, non-motile rods that occurred in chains. They were placed in the same subcluster based on 16S rRNA gene sequence analysis, phenotypic characteristics and levels of DNA–DNA relatedness. Their DNA G+C content ranged from 36 to 38 mol%. , and belong to cluster XVII of the subphylum. Strain ST18 was most closely related to ATCC 25536 in the phylogenetic tree, but these strains shared only 89.9 % (1336/1486 bp) 16S rRNA gene sequence similarity. , and are homofermentative bacteria, whereas ST18 produced CO from glucose. Whereas the cell-wall peptidoglycan type of and was -Lys–-Ala, that of and the subgroup represented by ST18 was A1 (-Ala–-Glu–-diaminopimelic acid). On the basis of 16S rRNA gene sequence divergence of more than 10 % from as well as phenotypic characteristics, strains ST18, HM244, HM250 and DI49 are considered to represent a novel species of a new genus belonging to the subphylum cluster XVII, for which the name gen. nov., sp. nov. is proposed. The type strain of is ST18 (=JCM 14210 =DSM 18934).


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vol. , part 12, pp. 2682 - 2686

Details of isolation of strains ST18 , HM244, HM250 and DI49

DNA–DNA relatedness between strain ST18 and related type strains

Phylogenetic relationships of strains ST18 , HM244, HM250 and DI49 with species in clusters XV–XIX of the subphylum based on 16S rRNA gene sequences. The trees were constructed using the maximum-parsimony method (S1) and the maximum-likelihood method (S2).

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