RT Journal Article SR Electronic(1) A1 Lee, Jeong-Eon A1 Seo, Jae Pyo A1 Lee, Dong Wan A1 Ko, Young-Hwan A1 Lee, Soon DongYR 2008 T1 Terrabacter lapilli sp. nov., an actinomycete isolated from stone JF International Journal of Systematic and Evolutionary Microbiology, VO 58 IS 5 SP 1084 OP 1088 DO https://doi.org/10.1099/ijs.0.65541-0 PB Microbiology Society, SN 1466-5034, AB A novel actinomycete, designated strain LR-26T, was isolated from a small stone collected from an agricultural field in Jeju, Republic of Korea. Cells of the organism were strictly aerobic, Gram-positive, non-motile, short rods. Colonies were bright yellow, circular, smooth and translucent. The organism was characterized chemotaxonomically as having ll-diaminopimelic acid in the cell wall, MK-8(H4) as major menaquinone, a polar lipid profile including diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and unknown phospholipids, iso-C15 : 0 as the predominant fatty acid and a DNA G+C content of 72.6 mol%. Comparative 16S rRNA gene sequence analysis showed that the organism was related to the genera Intrasporangium, Terracoccus and Terrabacter within the family Intrasporangiaceae. The closest phylogenetic relatives of strain LR-26T were the type strains of Terrabacter terrae (99.4 % 16S rRNA gene sequence similarity), Terrabacter aerolatus (99.3 %) and Terrabacter tumescens (99.3 %). DNA–DNA hybridization experiments showed that strain LR-26T shared low levels of DNA–DNA relatedness with Terrabacter terrae LMG 22921T (17.6 and 22.8 % from reciprocal experiments) and with Terrabacter tumescens IMSNU 21313T (27.6 and 34.9 %). The phenotypic data and low levels of DNA–DNA relatedness readily distinguished strain LR-26T from the type strains of recognized species of the genus Terrabacter, and showed that it therefore represents a novel species. The name Terrabacter lapilli sp. nov. is proposed for this novel actinomycete. The type strain is LR-26T (=JBRI 2002T =KCTC 19199T =DSM 18583T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.65541-0