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Abstract

A novel actinomycete, designated strain LR-26, was isolated from a small stone collected from an agricultural field in Jeju, Republic of Korea. Cells of the organism were strictly aerobic, Gram-positive, non-motile, short rods. Colonies were bright yellow, circular, smooth and translucent. The organism was characterized chemotaxonomically as having -diaminopimelic acid in the cell wall, MK-8(H) as major menaquinone, a polar lipid profile including diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and unknown phospholipids, iso-C as the predominant fatty acid and a DNA G+C content of 72.6 mol%. Comparative 16S rRNA gene sequence analysis showed that the organism was related to the genera , and within the family . The closest phylogenetic relatives of strain LR-26 were the type strains of (99.4 % 16S rRNA gene sequence similarity), (99.3 %) and (99.3 %). DNA–DNA hybridization experiments showed that strain LR-26 shared low levels of DNA–DNA relatedness with LMG 22921 (17.6 and 22.8 % from reciprocal experiments) and with IMSNU 21313 (27.6 and 34.9 %). The phenotypic data and low levels of DNA–DNA relatedness readily distinguished strain LR-26 from the type strains of recognized species of the genus , and showed that it therefore represents a novel species. The name sp. nov. is proposed for this novel actinomycete. The type strain is LR-26 (=JBRI 2002 =KCTC 19199 =DSM 18583).

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2008-05-01
2021-08-05
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vol. , part 5, pp. 1084 - 1088

Cellular fatty acid profiles of strain LR-26 and representatives of the genus . [PDF](38 KB)



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