1887

Abstract

A novel Gram-negative, rod-shaped, gliding bacterial strain designated LL04 12.1.7 was isolated from diseased sole ( Kaup) in Galicia, Spain. Colonies were yellow-pigmented with uneven edges and did not adhere to the agar. The DNA G+C content of the strain was 29.8 mol%. 16S rRNA gene sequence similarity analysis indicated that strain LL04 12.1.7 is a member of the genus in the family . Sequence similarities between the isolate and the type strains of other members of the genus were 96.7–94.8 %. The major fatty acids (>10 % of total fatty acids) were iso-C (23.1 %), iso-C 3-OH (10.6 %), C 6 (12.2 %) and summed feature 3 (comprising C 7 and/or iso-C 2-OH, 11.0 %). Genotypic and phenotypic data distinguished strain LL04 12.1.7 from the 11 recognized species, indicating that it represents a novel species, for which the name sp. nov. is proposed. The type strain is strain LL04 12.1.7 (=CECT 7292 =NCIMB 14368).

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2008-04-01
2019-08-18
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References

  1. Bernardet, J.-F., Segers, P., Vancanneyt, M., Berthe, F., Kersters, K. & Vandamme, P. ( 1996; ). Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46, 128–148.[CrossRef]
    [Google Scholar]
  2. Bernardet, J.-F., Nakagawa, Y. & Holmes, B. ( 2002; ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52, 1049–1070.[CrossRef]
    [Google Scholar]
  3. Buck, J. D. ( 1982; ). Non-staining (KOH) method for determination of gram reactions of marine bacteria. Appl Environ Microbiol 44, 992–993.
    [Google Scholar]
  4. Choi, D. H., Kim, Y.-G., Hwang, C. Y., Yi, H., Chun, J. & Cho, B. C. ( 2006; ). Tenacibaculum litoreum sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 56, 635–640.[CrossRef]
    [Google Scholar]
  5. Chun, J., Lee, J.-H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y. W. ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef]
    [Google Scholar]
  6. Cowan, S. T. & Steel, K. J. ( 1965; ). Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  7. Felsenstein, F. ( 2007; ). phylip (phylogeny inference package) version 3.67. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  8. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  9. Frette, L., Jørgensen, N. O. G., Irming, H. & Kroer, N. ( 2004; ). Tenacibaculum skagerrakense sp. nov., a marine bacterium isolated from the pelagic zone in Skagerrak, Denmark. Int J Syst Evol Microbiol 54, 519–524.[CrossRef]
    [Google Scholar]
  10. Hansen, G. H., Bergh, Ø., Michaelsen, J. & Knappskog, D. ( 1992; ). Flexibacter ovolyticus sp. nov., a pathogen of eggs and larvae of Atlantic halibut, Hippoglossus hippoglossus L. Int J Syst Bacteriol 42, 451–458.[CrossRef]
    [Google Scholar]
  11. Jung, S.-Y., Oh, T.-K. & Yoon, J.-H. ( 2006; ). Tenacibaculum aestuarii sp. nov., isolated from a tidal flat sediment in Korea. Int J Syst Evol Microbiol 56, 1577–1581.[CrossRef]
    [Google Scholar]
  12. Kimura, M. ( 1980; ). A simple model for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  13. Pazos, F., Santos, Y., Macías, A. R., Nuñez, S. & Toranzo, A. E. ( 1996; ). Evaluation of media for the successful culture of Flexibacter maritimus. J Fish Dis 19, 193–197.[CrossRef]
    [Google Scholar]
  14. Piñeiro-Vidal, M., Riaza, A. & Santos, Y. ( 2008; ). Tenacibaculum discolor sp. nov. and Tenacibaculum gallaicum sp. nov., isolated from sole (Solea senegalensis) and turbot (Psetta maxima) culture systems. Int J Syst Evol Microbiol 58, 21–25.[CrossRef]
    [Google Scholar]
  15. Reichenbach, H. ( 1989; ). Order I. Cytophagales Leadbetter 1974. In Bergey's Manual of Systematic Bacteriology, vol. 3, pp. 2011–2013. Edited by J. T. Staley, M. P. Bryant, N. Pfennig & J. G. Holt. Baltimore: Williams & Wilkins.
  16. Reichenbach, H. ( 1992a; ). The order Cytophagales. In The Prokaryotes, 2nd edn, vol. 4, pp. 3631–3675. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  17. Reichenbach, H. ( 1992b; ). Flavobacteriaceae fam. nov. In Validation of the Publication of the New Names and New Combinations Previously Effectively Published Outside the IJSB, List no. 41. Int J Syst Bacteriol 42, 327–329.
  18. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  19. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI.
  20. Sheu, S.-Y., Lin, K.-Y., Chou, J.-H., Chang, P.-S., Arun, A. B., Young, C.-C. & Chen, W.-M. ( 2007; ). Tenacibaculum litopenaei sp. nov., isolated from a shrimp mariculture pond. Int J Syst Evol Microbiol 57, 1148–1153.[CrossRef]
    [Google Scholar]
  21. Stackebrandt, E. & Ebers, J. ( 2006; ). Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33, 152–155.
    [Google Scholar]
  22. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  23. Suzuki, M., Nakagawa, Y., Harayama, S. & Yamamoto, S. ( 2001; ). Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum. Int J Syst Evol Microbiol 51, 1639–1652.[CrossRef]
    [Google Scholar]
  24. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  25. Wakabayashi, H., Hikida, M. & Masumura, K. ( 1986; ). Flexibacter maritimus sp. nov., a pathogen of marine fishes. Int J Syst Bacteriol 36, 396–398.[CrossRef]
    [Google Scholar]
  26. Yoon, J.-H., Kang, S.-J. & Oh, T.-K. ( 2005; ). Tenacibaculum lutimaris sp. nov., isolated from a tidal flat in the Yellow Sea, Korea. Int J Syst Evol Microbiol 55, 793–798.[CrossRef]
    [Google Scholar]
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