1887

International Journal of Systematic and Evolutionary Microbiology

Volume 58, Issue 7

sp. nov., isolated from fermented sausage (‘mum’) in Thailand Free

Abstract

A Gram-positive and catalase-negative coccus that formed chains, designated strain FP48-3, isolated from fermented sausage (‘mum’), was studied systematically. Strain FP48-3 was facultatively anaerobic and produced -lactic acid from glucose. Straight-chain fatty acids C and C were the dominant components. The DNA G+C content of strain FP48-3 was 37.9 mol%. On the basis of 16S rRNA and RNA polymerase -subunit () gene sequence analysis, strain FP48-3 was closely related to LMG 6399, LMG 10746 and LMG 11423, with 99.3–99.6 and 95.1–96.9 % sequence similarities, respectively. Strain FP48-3 could be clearly distinguished from LMG 6399, LMG 10746 and LMG 11423 by low DNA–DNA relatedness (≤14 %) and phenotypic characteristics. Therefore, this strain represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is FP48-3 (=KCTC 13134=NBRC 101867=NRIC 0107=TISTR 933=PCU 282).

  • Published Online:
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65535-0
2008-07-01
2020-08-13
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/7/1630.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65535-0&mimeType=html&fmt=ahah

References

  1. Baele, M., Baele, P., Vaneechoutte, M., Storms, V., Butaye, P., Devriese, L. A., Verschraegen, G., Gillis, M. & Haesebrouck, F. ( 2000; ). Application of tRNA intergenic spacer PCR for identification of Enterococcus species. J Clin Microbiol 38, 4201–4207.
     [Google Scholar]
  2. Barrow, G. I. & Feltham, R. K. A. ( 1993; ). Cowan and Steel's Manual for the Identification of Medical Bacteria, 3rd edn, pp. 331. Cambridge: Cambridge University Press.
  3. Cai, Y., Suyanandana, P., Saman, P. & Benno, Y. ( 1999; ). Classification and characterization of lactic acid bacteria isolated from the intestines of common carp and freshwater prawns. J Gen Appl Microbiol 45, 177–184.[CrossRef]
    [Google Scholar]
  4. Collins, M. D. & Jones, D. ( 1979; ). The distribution of isoprenoid quinones in streptococci of serological groups D and N. J Gen Microbiol 114, 27–33.[CrossRef]
    [Google Scholar]
  5. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E. ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef]
    [Google Scholar]
  6. Collins, M. D., Jones, D., Farrow, J. A. E., Kilpper-Bälz, R. & Schleifer, K. H. ( 1984; ). Enterococcus avium nom. rev., comb. nov.; E. casseliflavus nom. rev., comb. nov.; E. durans nom. rev., comb. nov.; E. gallinarum comb. nov.; and E. malodoratus sp. nov. Int J Syst Bacteriol 34, 220–223.[CrossRef]
    [Google Scholar]
  7. Collins, M. D., Farrow, J. A. E. & Jones, D. ( 1986; ). Enterococcus mundtii sp. nov. Int J Syst Bacteriol 36, 8–12.[CrossRef]
    [Google Scholar]
  8. De Graef, E. M., Devriese, L. A., Vancanneyt, M., Baele, M., Collins, M. D., Lefebvre, K., Swings, J. & Haesbrouck, F. ( 2003; ). Description of Enterococcus canis sp. nov. from dogs and reclassification of Enterococcus porcinus Teixeira et al. 2001 as a later synonym of Enterococcus villorum Vancanneyt et al. Int J Syst Evol Microbiol 53, 1069–1074.[CrossRef]
    [Google Scholar]
  9. Euzéby, J. P. ( 1997; ). List of bacterial names with standing in nomenclature: a folder available on the Internet. Int J Syst Bacteriol 47, 590–592.[CrossRef]
    [Google Scholar]
  10. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  11. Farrow, J. A. E. & Collins, M. D. ( 1985; ). Enterococcus hirae, a new species that includes amino acid assay strain NCDO 1258 and strains causing growth depression in young chickens. Int J Syst Bacteriol 35, 73–75.[CrossRef]
    [Google Scholar]
  12. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  13. Felsenstein, J. ( 1989; ). phylip – phylogeny inference package (version 3.2). Cladistics 5, 164–166.
     [Google Scholar]
  14. Hall, T. A. ( 1999; ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41, 95–98.
     [Google Scholar]
  15. Hardie, J. M. & Whiley, R. A. ( 1997; ). Classification and overview of the genera Streptococcus and Enterococcus. J Appl Microbiol 83 (Suppl.), 1S–11S.[CrossRef]
    [Google Scholar]
  16. Hucker, G. J. & Conn, H. J. ( 1923; ). Method of Gram staining. N Y St Agric Exp Sta Tech Bull 93, 3–37.
     [Google Scholar]
  17. Ikemoto, S., Katoh, K. & Komagata, K. ( 1978; ). Cellular fatty acid composition in methanol-utilizing bacteria. J Gen Appl Microbiol 24, 41–49.[CrossRef]
    [Google Scholar]
  18. Kihara, H. & Snell, E. E. ( 1960; ). Peptides and bacterial growth. J Biol Chem 235, 1409–1414.
     [Google Scholar]
  19. Knudtson, L. M. & Hartman, P. A. ( 1992; ). Routine procedures for isolation and identification of enterococci and faecal streptococci. Appl Environ Microbiol 58, 3027–3031.
     [Google Scholar]
  20. Lane, D. J. ( 1991; ). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. Chichester: Wiley.
  21. Merquior, V. L. C., Peralta, J. M., Facklam, R. R. & Teixeira, L. M. ( 1994; ). Analysis of electrophoretic whole-cell protein profiles as a tool for characterization of Enterococcus species. Curr Microbiol 28, 149–153.[CrossRef]
    [Google Scholar]
  22. Naser, S. M., Thompson, F. L., Hoste, B., Gevers, D., Dawyndt, P., Vancanneyt, M. & Swings, J. ( 2005; ). Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes. Microbiology 151, 2141–2150.[CrossRef]
    [Google Scholar]
  23. Naser, S. M., Vancanneyt, M., Hoste, B., Snauwaert, C., Vandemeulebroecke, K. & Swings, J. ( 2006; ). Reclassification of Enterococcus flavescens Pompei et al. 1992 as a later synonym of Enterococcus casseliflavus (ex Vaughan et al. 1979) Collins et al. 1984 and Enterococcus saccharominimus Vancanneyt et al. 2004 as a later synonym of Enterococcus italicus Fortina et al. 2004. Int J Syst Evol Microbiol 56, 413–416.[CrossRef]
    [Google Scholar]
  24. Okada, S., Toyoda, T. & Kozaki, M. ( 1978; ). An easy method for the determination of the optical types of lactic acid produced by lactic acid bacteria. Agric Biol Chem 42, 1781–1783.[CrossRef]
    [Google Scholar]
  25. Perrière, G. & Gouy, M. ( 1996; ). WWW-Query: an on-line retrieval system for biological sequence banks. Biochimie 78, 364–369.[CrossRef]
    [Google Scholar]
  26. Saito, H. & Miura, K. ( 1963; ). Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72, 619–629.[CrossRef]
    [Google Scholar]
  27. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
     [Google Scholar]
  28. Sukontasing, S., Tanasupawat, S., Moonmangmee, S., Lee, J.-S. & Suzuki, K. I. ( 2007; ). Enterococcus camelliae sp. nov., isolated from fermented tea leaves in Thailand. Int J Syst Evol Microbiol 57, 2151–2154.[CrossRef]
    [Google Scholar]
  29. Švec, P., Vancanneyt, M., Sedláček, I., Naser, S. M., Snauwaert, C., Lefebvre, K., Hoste, B. & Swings, J. ( 2006; ). Enterococcus silesiacus sp. nov. and Enterococcus termitis sp. nov. Int J Syst Evol Microbiol 56, 577–581.[CrossRef]
    [Google Scholar]
  30. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  31. Tamaoka, J., Katayama-Fujimura, Y. & Kuraishi, H. ( 1983; ). Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. J Appl Bacteriol 54, 31–36.[CrossRef]
    [Google Scholar]
  32. Tanasupawat, S., Ezaki, T., Suzuki, K., Okada, S., Komagata, K. & Kozaki, M. ( 1992; ). Characterization and identification of Lactobacillus pentosus and Lactobacillus plantarum strains from fermented foods in Thailand. J Gen Appl Microbiol 38, 121–134.[CrossRef]
    [Google Scholar]
  33. Teixeira, L. M., Carvaho, M. G., Espinola, M. M. B., Steigerwalt, A. G., Douglas, M. P., Brenner, D. J. & Facklam, R. R. ( 2001; ). Enterococcus porcinus sp. nov. and Enterococcus ratti sp. nov., associated with enteric disorders in animals. Int J Syst Evol Microbiol 51, 1737–1743.[CrossRef]
    [Google Scholar]
  34. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  35. Vancanneyt, M., Snauwaert, C., Cleenwerck, I., Baele, M., Descheemaeker, P., Goossens, H., Pot, B., Vandamme, P., Swings, J. & other authors ( 2001; ). Enterococcus villorum sp. nov., an enteroadherent bacterium associated with diarrhoea in piglets. Int J Syst Evol Microbiol 51, 393–400.
     [Google Scholar]
  36. Whittenbury, R. ( 1963; ). The use of soft agar in the study of condition affecting the utilization of fermentable substrates by lactic acid bacteria. J Gen Microbiol 32, 375–384.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65535-0
Loading
Enterococcus thailandicus sp. nov., isolated from fermented sausage (‘mum’) in Thailand
Int J Syst Evol Microbiol 58, 1630 (2008); https://doi.org/10.1099/ijs.0.65535-0
/content/journal/ijsem/10.1099/ijs.0.65535-0
/content/journal/ijsem/10.1099/ijs.0.65535-0
Loading

Data & Media loading...

Supplements

Neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showing the relationships between strain FP48-3 ( sp. nov.) and related species. [PDF](25 KB)

PDF

Neighbour-joining phylogenetic tree based on gene sequences showing the relationships between strain FP48-3 ( sp. nov.) and related species. [PDF](25 KB)

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error