A Gram-positive, rod-shaped bacterium, designated strain LBY, was isolated from the blood of a Korean patient. The organism could not be identified based on conventional clinical microbiological methods. 16S rRNA gene sequencing and phylogenetic analysis revealed that it belonged to the genus , but was distinct from recognized species. Strain LBY was related most closely to the type strains of and , with 16S rRNA gene sequence similarities of 96.2 and 95.4 %, respectively. The major fatty acids of strain LBY were anteiso-C (43.8 %), C (10.4 %), iso-C (10.3 %), iso-C (9.2 %) and anteiso-C (8.3 %). On the basis of biochemical characteristics, fatty acid composition and comparative 16S rRNA gene sequence analysis, strain LBY is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is LBY (=ABB-ID-KSY9=KCTC 13165=JCM 14798).


Article metrics loading...

Loading full text...

Full text loading...



  1. Ash, C., Priest, F. G. & Collins, M. D.(1993). Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus. Antonie van Leeuwenhoek 64, 253–260. [Google Scholar]
  2. Daane, L. L., Harjono, I., Barns, S. M., Launen, L. A., Palleroni, N. J. & Häggblom, M. M.(2002). PAH-degradation by Paenibacillus spp. and description of Paenibacillus naphthalenovorans sp. nov., a naphthalene-degrading bacterium from the rhizosphere of salt marsh plants. Int J Syst Evol Microbiol 52, 131–139. [Google Scholar]
  3. Djordjevic, S. P., Forbes, W. A., Smith, L. A. & Hornitzky, M. A.(2000). Genetic and biochemical diversity among isolates of Paenibacillus alvei cultured from Australian honeybee (Apis mellifera) colonies. Appl Environ Microbiol 66, 1098–1106.[CrossRef] [Google Scholar]
  4. Heyndrickx, M., Vandemeulebroecke, K., Scheldeman, P., Kersters, K., De Vos, P., Logan, N. A., Aziz, A. M. & Berkeley, R. C. W.(1996). A polyphasic reassessment of the genus Paenibacillus, reclassification of Bacillus lautus (Nakamura 1984) as Paenibacillus lautus comb. nov., and of Bacillus peoriae (Montefusco et al. 1993) as Paenibacillus peoriae comb. nov., and emended descriptions of P. lautus and P. peoriae. Int J Syst Bacteriol 46, 988–1003.[CrossRef] [Google Scholar]
  5. Horn, M. A., Ihssen, J., Matthies, C., Schramm, A., Acker, G. & Drake, H. L.(2005).Dechloromonas dentrificans sp. nov., Flavobacterium dentrificans sp. nov., Paenibacillus anaericanus sp. nov. and Paenibacillus terrae strain MN72, N2O-producing bacteria isolated from the gut of the earthworm Aporrectodea caliginosa. Int J Syst Evol Microbiol 55, 1255–1265.[CrossRef] [Google Scholar]
  6. Ko, K. S., Oh, W. S., Lee, M. Y., Peck, K. R., Lee, N. Y. & Song, J.-H.(2007). A new Microbacterium species isolated from the blood of a patient with fever: Microbacterium pyrexiae sp. nov. Diagn Microbiol Infect Dis 57, 393–397.[CrossRef] [Google Scholar]
  7. Logan, N. A. & Turnbull, P. C. B.(2003).Bacillus and other aerobic endospore-forming bacteria. In Manual of Clinical Microbiology, 8th edn, pp. 445–460. Edited by P. R. Murray. Washington, DC: American Society for Microbiology.
  8. Marmur, J. & Doty, P.(1962). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef] [Google Scholar]
  9. Roux, V. & Raoult, D.(2004).Paenibacillus massiliensis sp. nov., Paenibacillus sanguinis sp. nov. and Paenibacillus timonensis sp. nov., isolated from blood cultures. Int J Syst Evol Microbiol 54, 1049–1054.[CrossRef] [Google Scholar]
  10. Stackebrandt, E. & Goebel, B. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef] [Google Scholar]
  11. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  12. Zhu, X. Y., Zhong, T., Pandva, Y. & Joerger, R. D.(2002). 16S rRNA-based analysis of microbiota from the cecum of broiler chickens. Appl Environ Microbiol 68, 124–137.[CrossRef] [Google Scholar]

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error