The taxonomic position of two soil isolates, 3-46-b(3) and 3-45-a(11), was clarified based on data from a polyphasic study. The organisms showed a combination of chemotaxonomic and morphological properties typical of the genus . They formed distinct phyletic lines in the 16S rRNA gene tree and were closely associated with the type strains of (sequence similarity of 98.3–98.5 %), (98.1–98.3 %) and (96.4–96.8 %). The level of 16S rRNA gene sequence similarity between the new isolates was 99.1 %. The level of DNA–DNA hybridization between strains 3-46-b(3) and 3-45-a(11) was 43.6 % and levels of relatedness between the two new isolates and the type strains of and were 21.0–27.3 %. On the basis of phenotypic and genotypic properties, the new isolates could be differentiated from each other and from their closest phylogenetic relatives. It is proposed that the organisms be classified as representing two novel species of the genus . The names proposed for these taxa are sp. nov. [type strain 3-46-b(3) =JCM 13933 =MTCC8057] and sp. nov. [type strain 3-45-a(11) =JCM 13932 =MTCC8056].


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vol. , part 7, pp. 1653 - 1659

Neighbour-joining tree based on nearly complete 16S rRNA gene sequences showing the relationship between strains 3-46-b(3) and 3-45-a(11) and the type strains of all species of the genus in the family . [PDF](22 KB)


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