Three Gram-positive, aerobic, non-motile, non-acid–alcohol-fast strains, designated I05-00051, I05-00074 and I03-00808, were isolated from different soil samples in Beijing and Sichuan, China. Phylogenetic analysis based on 16S rRNA gene sequences and DNA–DNA hybridization experiments revealed that these three isolates represented the same genospecies. These three strains showed <97.0 % 16S rRNA gene sequence similarity with the type strains of recognized species of the genus , with the exception of subsp. DSM 43463 (98.1 % gene sequence similarity) and DSM 44228 (98.0 % similarity). Chemotaxonomic data, including -diaminopimelic acid as the diagnostic diamino acid, arabinose and galactose as predominant sugars, iso-C, iso-C, iso-C and anteiso-C as major fatty acids, MK-9(H) as predominant menaquinone and polar lipids dominated by diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol, supported the affiliation of these three organisms to the genus . The genomic DNA G+C contents of the three isolates were 68.2–69.9 mol%. The results of DNA–DNA hybridization experiments among these three isolates and subsp. DSM 43463 and DSM 44228, in combination with chemotaxonomic and physiological data, demonstrated that the three new isolates represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is I05-00074 (=CCM 7463=KCTC 19303).


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vol. , part 5, pp. 1180 - 1185

Cellular fatty acid content (% of total) of strains I05-00074 , I05-00051 and I03-00808.

DNA-DNA relatedness (%) among the three tested strains and subsp. DSM 43463 and DSM 44228 .

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