1887

Abstract

The taxonomic position of a Gram-positive coccus, designated strain CAU 28, isolated from activated sludge foam was determined by using a polyphasic approach. Based on its cellular morphology and the results of biochemical tests, strain CAU 28 was identified tentatively as a member of the genus . Comparative 16S rRNA gene sequence analysis showed that levels of similarity between strain CAU 28 and the type strains of recognized species ranged from 90.4 to 97.2 %. DNA–DNA hybridization studies showed that strain CAU 28 displayed less than 26.1 % relatedness to the type strains of recognized species. The rep-PCR fingerprints revealed that strain CAU 28 was well separated from reference species. The combined genotypic and phenotypic data indicate that strain CAU 28 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CAU 28 (=KCTC 13185 =CCUG 55099).

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2008-08-01
2020-01-23
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References

  1. Carson, J., Gudkovs, N. & Austin, B. ( 1993; ). Characteristics of an enterococcus-like bacterium from Australia and South Africa, pathogenic for rainbow trout, Oncorhynchus mykiss (Walbaum). J Fish Dis 16, 381–388.[CrossRef]
    [Google Scholar]
  2. Chen, S. C., Lin, Y. D., Liaw, L. L. & Wang, P. C. ( 2001; ). Lactococcus garvieae infection in the giant freshwater prawn Macrobrachium rosenbergii confirmed by polymerase chain reaction and 16S rDNA sequencing. Dis Aquat Organ 45, 45–52.[CrossRef]
    [Google Scholar]
  3. Chen, S. C., Liaw, L. L., Su, H. Y., Ko, S. C., Wu, C. Y., Chaung, H. C., Tsai, Y. H., Yang, K. L., Chen, Y. C. & other authors ( 2002; ). Lactococcus garvieae, a cause of disease in grey mullet, Mugil cephalus L., in Taiwan. J Fish Dis 25, 727–732.[CrossRef]
    [Google Scholar]
  4. Collins, M. D., Farrow, J. A. E., Phillips, B. A. & Kandler, O. ( 1983; ). Streptococcus garvieae sp. nov. and Streptococcus plantarum sp. nov. J Gen Microbiol 129, 3427–3431.
    [Google Scholar]
  5. Euzéby, J. P. ( 1997; ). List of Bacterial Names with Standing in Nomenclature: a folder available on the Internet. Int J Syst Bacteriol 47, 590–592.[CrossRef]
    [Google Scholar]
  6. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  7. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  8. Fitch, W. M. & Margoliash, E. ( 1967; ). Construction of phylogenetic trees: a method based on mutation distances as estimated from cytochrome c sequences is of general applicability. Science 155, 279–284.[CrossRef]
    [Google Scholar]
  9. Gordon, R. E. & Mihm, J. M. ( 1962; ). Identification of Nocardia caviae (Erikson) nov. comb. Ann N Y Acad Sci 98, 628–636.
    [Google Scholar]
  10. Goris, J., Suzuki, K., De Vos, P., Nakase, T. & Kersters, K. ( 1998; ). Evaluation of a microplate DNA-DNA hybridization method compared with the initial renaturation method. Can J Microbiol 44, 1148–1153.[CrossRef]
    [Google Scholar]
  11. Mannion, P. T. & Rothburn, M. M. ( 1990; ). Diagnosis of bacterial endocarditis by Streptococcus lactis and assisted by immunoblotting of serum antibodies. J Infect 21, 317–326.[CrossRef]
    [Google Scholar]
  12. Minnikin, D. E., Hutchinson, I. G., Caldicott, A. B. & Goodfellow, M. ( 1980; ). Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188, 221–233.[CrossRef]
    [Google Scholar]
  13. Nam, S. W., Kim, W., Chun, J. & Goodfellow, M. ( 2004; ). Tsukamurella pseudospumae sp. nov., a novel actinomycete isolated from activated sludge foam. Int J Syst Evol Microbiol 54, 1209–1212.[CrossRef]
    [Google Scholar]
  14. Pereira, F., Ravelo, C., Toranzo, A. E. & Romalde, J. L. ( 2004; ). Lactococcus garvieae, an emerging pathogen for the Portuguese trout culture. Bull Eur Assoc Fish Pathol 24, 274–279.
    [Google Scholar]
  15. Rajashekara, G., Koeuth, T., Nevile, S., Back, A., Nagaraja, K. V., Lupski, J. R. & Kapur, V. ( 1998; ). SERE, a widely dispersed bacterial repetitive DNA element. J Med Microbiol 47, 489–498.[CrossRef]
    [Google Scholar]
  16. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  17. Salminen, S., von Wright, A., Morelli, L., Marteau, P., Brassart, D., de Vos, W. M., Fondén, R., Saxelin, M., Collins, K. & other authors ( 1998; ). Demonstration of safety of probiotics – a review. Int J Food Microbiol 44, 93–106.[CrossRef]
    [Google Scholar]
  18. Schleifer, K. H., Kraus, J., Dvorak, C., Kilpper-Bälz, R., Collins, M. D. & Fischer, W. ( 1985; ). Transfer of Streptococcus lactis and related streptococci to the genus Lactococcus gen. nov. Syst Appl Microbiol 6, 183–195.[CrossRef]
    [Google Scholar]
  19. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  20. Stackebrandt, E., Frederiksen, W., Garrity, G. M., Grimont, P. A. D., Kämpfer, P., Maiden, M. C. J., Nesme, X., Rosselló-Mora, R., Swings, J. & other authors ( 2002; ). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52, 1043–1047.[CrossRef]
    [Google Scholar]
  21. Teixeira, L. M., Merquior, V. L., Vianni, M. C. E., Carvalho, M. G. S., Fracalanzza, S. E. L., Steigerwalt, A. G., Brenner, D. J. & Facklam, R. R. ( 1996; ). Phenotypic and genotypic characterization of atypical Lactococcus garvieae strains isolated from water buffalos with subclinical mastitis and confirmation of L. garvieae as a senior subjective synonym of Enterococcus seriolicida. Int J Syst Bacteriol 46, 664–668.[CrossRef]
    [Google Scholar]
  22. Vendrell, D., Balcázar, J. L., Ruiz-Zarzuela, I., de Blas, I., Gironés, O. & Múzquiz, J. L. ( 2006; ). Lactococcus garvieae in fish: a review. Comp Immunol Microbiol Infect Dis 29, 177–198.[CrossRef]
    [Google Scholar]
  23. Versalovic, J., Koeuth, T. & Lupski, J. R. ( 1991; ). Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res 19, 6823–6831.[CrossRef]
    [Google Scholar]
  24. Versalovic, J., Schneider, M., de Bruijn, F. J. & Lupski, J. R. ( 1994; ). Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction. Methods Mol Cell Biol 5, 25–40.
    [Google Scholar]
  25. Vinh, D. C., Nichol, K. A., Rand, F. & Embil, J. M. ( 2006; ). Native-valve bacterial endocarditis caused by Lactococcus garvieae. Diagn Microbiol Infect Dis 56, 91–94.[CrossRef]
    [Google Scholar]
  26. Wang, C. Y., Shie, H. S., Chen, S. C., Huang, J. P., Hsieh, I. C., Wen, M. S., Lin, F. C. & Wu, D. ( 2007; ). Lactococcus garvieae infections in humans: possible association with aquaculture outbreaks. Int J Clin Pract 61, 68–73.
    [Google Scholar]
  27. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  28. Yiu, K. H., Siu, C. W., To, K. K. W., Jim, M. H., Lee, K. L. F., Lau, C. P. & Tse, H. F. ( 2007; ). A rare cause of infective endocarditis; Lactococcus garvieae. Int J Cardiol 114, 286–287.[CrossRef]
    [Google Scholar]
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