A novel actinomycete strain, DAS-139, was isolated from a soil sample collected from Gulbarga, Karnataka Province, India. The isolate was characterized by white to grey aerial mycelium. Long spore chains were found on the aerial mycelium and the aerial mycelium was composed of non-motile spores with hairy surfaces. The cell wall of strain DAS-139 contained -diaminopimelic acid isomer as the diagnostic diaminoacid indicating that the cell wall was of chemotype-I. The predominant menaquinones were MK-9(H) (76 %), MK-9(H) (14 %) and MK-9(H) (10 %). Phosphatidylethanolamine was the diagnostic phospholipid. On the basis of 16S rRNA gene sequence, phenotypic and phylogenetic analyses, the novel strain was identified as a member of the genus . The novel strain grew optimally at 28 °C and pH 9.0. The GC content of the genomic DNA was 71.8 mol%. 16S rRNA gene sequence analysis showed that the novel isolate had 99.4 % sequence similarity with ATCC 49173 and 99.2 % similarity with ATCC 12309. Furthermore, DNA–DNA hybridization with these two species showed 36.0 and 43.0 % relatedness, respectively. Based on these observations, strain DAS-139 is proposed to represent a novel species of the genus , for which the name sp. nov. is proposed with the type strain DAS-139 (=KCTC 19241=CCTCC AA 207004).


Article metrics loading...

Loading full text...

Full text loading...



  1. Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J.(1997). Gapped blast and PSI_BLAST: a new generation of protein database search programs. Nucleic Acids Res 25, 3389–3402.[CrossRef] [Google Scholar]
  2. Collins, M. D.(1985). Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by M. Goodfellow & D. E. Minnikin. London: Academic Press.
  3. Cui, X. L., Mao, P. H., Tseng, M., Li, W. J., Zhang, L. P., Xu, L. H. & Jiang, C. L.(2001).Streptomonospora gen. nov., a new member of the family Nocardiopsaceae. Int J Syst Evol Microbiol 51, 357–363. [Google Scholar]
  4. De Ley, J., Cattoir, H. & Reynaerts, A.(1970). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef] [Google Scholar]
  5. Felsenstein, J.(1981). Evolutionary tree from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef] [Google Scholar]
  6. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  7. Fitch, W. M. & Margoliash, E.(1967). Construction of phylogenetic trees: a method based on mutation distances as estimated from cytochrome c sequences is of general applicability. Science 155, 279–284.[CrossRef] [Google Scholar]
  8. Goodfellow, M. & Cross, T.(1984). In Biology of the Actinomycetes, pp. 7–164. Edited by M. Goodfellow, M. Mordarski & S. T. Williams. London: Academic Press.
  9. Hain, T., Ward-Rainey, N., Kroppenstedt, R. M., Stackebrandt, E. & Rainey, F. A.(1997). Discrimination of Streptomyces albidoflavus strains based on the size and number of 16S–23S ribosomal DNA intergenic spacers. Int J Syst Bacteriol 47, 202–206.[CrossRef] [Google Scholar]
  10. Huß, V. A. R., Festl, H. & Schleifer, K. H.(1983). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef] [Google Scholar]
  11. Jahnke, K. D.(1992).basic computer program for evaluation of spectroscopic DNA renaturation data from GILFORD SYSTEM 2600 spectrophotometer on a PC/XT/AT type personal computer. J Microbiol Methods 15, 61–73.[CrossRef] [Google Scholar]
  12. Jiang, L.-Y., Li, M.-G., Li, W.-J., Cui, X.-L., Xu, L.-H. & Jiang, C.-L.(2001). Study on the application of quantitative analysis of cell-wall amino acids in actinomycetes classification. Wei Sheng Wu Xue Bao 41, 270–277 (in Chinese). [Google Scholar]
  13. Kelly, K. L.(1964). Inter-Society Color Council–National Bureau of Standard Color-name charts illustrated with centroid colors. US Government Printing Office, Washington.
  14. Kim, S. B. & Goodfellow, M.(2002).Streptomyces avermitilis sp. nov., nom. rev., a taxonomic home for the avermectin-producing streptomycetes. Int J Syst Evol Microbiol 52, 2011–2014.[CrossRef] [Google Scholar]
  15. Kim, S. B., Falconer, C., Williams, E. & Goodfellow, M.(1998).Streptomyces thermocarboxydovorans sp. nov. and Streptomyces thermocarboxydus sp. nov., two moderately thermophilic carboxydotrophic species from soil. Int J Syst Bacteriol 48, 59–68.[CrossRef] [Google Scholar]
  16. Kim, S. B., Al-Tai, A. M., Kim, S. B., Somasundaram, P. & Goodfellow, M.(2000).Streptomyces thermocoprophilus sp. nov., a cellulose-free endo-xylanase-producing streptomycete. Int J Syst Evol Microbiol 50, 505–509.[CrossRef] [Google Scholar]
  17. Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequence. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  18. Kluge, A. G. & Farris, F. S.(1969). Quantitative phyletics and the evolution of anurans. Syst Zool 18, 1–32.[CrossRef] [Google Scholar]
  19. Komagata, K. & Suzuki, K.-I.(1987). Lipid and cell wall analysis in bacterial systematics. In Methods in Microbiology, vol. 19, pp. 161–207. London: Academic Press.
  20. Kroppenstedt, R. M.(1985). Fatty acid and menaquinones analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics, pp. 173–199. Edited by M. Goodfellow & D. E. Minnikin. London: Academic Press.
  21. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M.(2001).mega2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef] [Google Scholar]
  22. Lechevalier, H. A. & Lechevalier, M. P.(1970a). A critical evaluation of the genera of aerobic actinomycetes. In The Actinomycetales, pp. 393–405. Edited by H. Prauser. Jena: VEB Gustav Fischer.
  23. Lechevalier, M. P. & Lechevalier, H. A.(1970b). Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20, 435–443.[CrossRef] [Google Scholar]
  24. Lechevalier, M. P. & Lechevalier, H. A.(1980). The chemotaxonomy of actinomycetes. In Actinomycete Taxonomy, pp. 22. Edited by Dietz, Thayer. Arlington, VA: Society for Industrial Microbiology.
  25. Lechevalier, M. P., De Bièvre, C. & Lechevalier, H. A.(1977). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5, 249–260.[CrossRef] [Google Scholar]
  26. Li, W., Lanoot, B., Zhang, Y., Vancanneyt, M., Swings, J. & Liu, Z.(2002).Streptomyces scopiformis sp. nov., a novel streptomycete with fastigiate spore chains. Int J Syst Evol Microbiol 52, 1629–1633.[CrossRef] [Google Scholar]
  27. Marmur, J. & Doty, P.(1962). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef] [Google Scholar]
  28. Minnikin, D. E., Hutchinson, I. G., Caldicott, A. B. & Goodfellow, M.(1980). Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188, 221–223.[CrossRef] [Google Scholar]
  29. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H.(1984). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef] [Google Scholar]
  30. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  31. Shirling, E. B. & Gottlieb, D.(1966). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef] [Google Scholar]
  32. Shirling, E. B. & Gottlieb, D.(1968a). Cooperative description of type cultures of Streptomyces. II. Species descriptions from first study. Int J Syst Bacteriol 18, 69–189.[CrossRef] [Google Scholar]
  33. Shirling, E. B. & Gottlieb, D.(1968b). Cooperative description of type cultures of Streptomyces. III. Additional species descriptions from first and second studies. Int J Syst Bacteriol 18, 279–392.[CrossRef] [Google Scholar]
  34. Shirling, E. B. & Gottlieb, D.(1969). Cooperative description of type cultures of Streptomyces. IV. Species descriptions from the second, third and fourth studies. Int J Syst Bacteriol 19, 391–512.[CrossRef] [Google Scholar]
  35. Stackebrandt, E.(1986). The significance of wall type in phylogenetically based taxonomic studies on actinomyctes. In Biological, Biochemical and Biomedical Aspects of Actinomycetes, Part B, pp. 497–506. Edited by G. Szabo, S. Biro & M. Goodfellow. Budapest: Academia Kiado.
  36. Takeuchi, M. & Hatano, K.(1998). Union of the genera Microbacterium Orla-Jensen and Aureobacterium Collins et al. in a redefined genus Microbacterium. Int J Syst Bacteriol 48, 739–749.[CrossRef] [Google Scholar]
  37. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4888.[CrossRef] [Google Scholar]
  38. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murry, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  39. Williams, S. T., Goodfellow, M., Alderson, G., Wellington, E. M. H., Sneath, P. H. A. & Sackin, M. J.(1983). Numerical classification of Streptomyces and related genera. J Gen Microbiol 129, 1743–1813. [Google Scholar]

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error