1887

Abstract

A novel actinomycete strain, DAS-139, was isolated from a soil sample collected from Gulbarga, Karnataka Province, India. The isolate was characterized by white to grey aerial mycelium. Long spore chains were found on the aerial mycelium and the aerial mycelium was composed of non-motile spores with hairy surfaces. The cell wall of strain DAS-139 contained -diaminopimelic acid isomer as the diagnostic diaminoacid indicating that the cell wall was of chemotype-I. The predominant menaquinones were MK-9(H) (76 %), MK-9(H) (14 %) and MK-9(H) (10 %). Phosphatidylethanolamine was the diagnostic phospholipid. On the basis of 16S rRNA gene sequence, phenotypic and phylogenetic analyses, the novel strain was identified as a member of the genus . The novel strain grew optimally at 28 °C and pH 9.0. The GC content of the genomic DNA was 71.8 mol%. 16S rRNA gene sequence analysis showed that the novel isolate had 99.4 % sequence similarity with ATCC 49173 and 99.2 % similarity with ATCC 12309. Furthermore, DNA–DNA hybridization with these two species showed 36.0 and 43.0 % relatedness, respectively. Based on these observations, strain DAS-139 is proposed to represent a novel species of the genus , for which the name sp. nov. is proposed with the type strain DAS-139 (=KCTC 19241=CCTCC AA 207004).

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2008-05-01
2019-12-13
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