A Gram-negative, rod-shaped, moderately halophilic bacterium, designated strain YIM 91118T, motile with a single polar flagellum, was isolated from Xinjiang province in north-west China and subjected to a polyphasic taxonomic study. Strain YIM 91118T grew optimally at 37 °C, pH 7.0–8.0 and 10 % NaCl (w/v). Its major cellular fatty acids were iso-C15 : 0, iso-C17 : 0, C16 : 0 and iso-C17 : 1ω9c. The predominant lipoquinone was Q-8. The DNA G+C content was 63.2 mol%. All of these chemotaxonomic data supported the assignment of the new isolate to the genus Microbulbifer. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 91118T belongs to the genus Microbulbifer and it formed a distinct subclade with Microbulbifer maritimus KCCM 41774T. The levels of 16S rRNA gene sequence similarity to the type strains of Microbulbifer species were in the range 93.5–95.0 %. The mean level of DNA–DNA relatedness between strain YIM 91118T and M. maritimus KCCM 41774T was 45.8 %. On the basis of phenotypic properties, phylogeny and genomic data, strain YIM 91118T represents a novel species of the genus Microbulbifer, for which the name Microbulbifer halophilus sp. nov. is proposed. The type strain is YIM 91118T (=CCTCC AB 206094T =KCTC 12848T). Furthermore, it is necessary to emend the description of the genus Microbulbifer.
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International Journal of Systematic and Evolutionary
Microbiology vol.
58 , part 9, pp. 2036 - 2040
Supplementary Fig. S1. Transmission electron
micrograph of a cell of strain YIM 91118
Tgrown on modified ISP 5 agar medium supplemented
with 10% (w/v) NaCl at 37°C for 2 days. Bar, 500 nm.