Strain PN5 is a Gram-positive, aerobic, motile, rod-shaped, peritrichously flagellated bacterium that was isolated from the Pindari glacier using nutrient agar medium. Cells of PN5 are catalase-positive and oxidase-negative and contain lysine, glutamic acid and alanine in the peptidoglycan (peptidoglycan type A4). Further, the cells are characterized by the presence of iso-C and iso-C as the predominant fatty acids and MK-7 as the isoprenoid quinone. Based on the above characteristics, strain PN5 was assigned to the genus . Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain PN5 clustered with the type strain of with a sequence similarity of 97.2 %. DNA–DNA hybridization between PN5 and DSM 12223 resulted in a relatedness of only 15 %, clearly indicating that strain PN5 represents a novel species. Further, PN5 was different from with respect to various phenotypic and chemotaxonomic characteristics. Therefore, strain PN5 is identified as a representative of a novel species of the genus , for which the name sp. nov. is proposed. is unique among psychrotolerant species in containing -Lys–-Glu in the cell-wall peptidoglycan. The type strain is PN5 (=LMG 23935 =MTCC9127 =JCM 15113).


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  1. Abd El-Rahman, H. A., Fritze, D., Spröer, C. & Claus, D.(2002). Two novel psychrotolerant species, Bacillus psychrotolerans sp. nov. and Bacillus psychrodurans sp. nov., which contain ornithine in their cell walls. Int J Syst Evol Microbiol 52, 2127–2133.[CrossRef] [Google Scholar]
  2. Albert, R. A., Archambault, J., Rosselló-Mora, R., Tindall, B. J. & Matheny, M.(2005).Bacillus acidicola sp. nov., a novel mesophilic, acidophilic species isolated from acidic Sphagnum peat bogs in Wisconsin. Int J Syst Evol Microbiol 55, 2125–2130.[CrossRef] [Google Scholar]
  3. Bae, S. S., Lee, J. H. & Kim, S. J.(2005).Bacillus alveayuensis sp. nov., a thermophilic bacterium isolated from deep-sea sediments of the Ayu Trough. Int J Syst Evol Microbiol 55, 1211–1215.[CrossRef] [Google Scholar]
  4. Baker, G. C., Gaffar, S., Cowan, D. A. & Suharto, A. R.(2001). Bacterial community analysis of Indonesian hot springs. FEMS Microbiol Lett 200, 103–109.[CrossRef] [Google Scholar]
  5. Brambilla, E., Hippe, H., Hagelstein, A., Tindall, B. J. & Stackebrandt, E.(2001). 16S rDNA diversity of cultured and uncultured prokaryotes of a mat sample from Lake Fryxell, McMurdo Dry Valleys, Antarctica. Extremophiles 5, 23–33.[CrossRef] [Google Scholar]
  6. Caccamo, D., Gugliandolo, C., Stackebrandt, E. & Maugeri, T. L.(2000).Bacillus vulcani sp. nov., a novel thermophilic species isolated from a shallow marine hydrothermal vent. Int J Syst Evol Microbiol 50, 2009–2012.[CrossRef] [Google Scholar]
  7. Claus, D. & Berkeley, R. C. W.(1986). Genus Bacillus Cohn 1872, 174AL. In Bergey's Manual of Systematic Bacteriology, vol. 2, pp. 1105–1139. Edited by P. H. A. Sneath, N. S. Mair, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  8. Cohn, F.(1872). Untersuchungen über Bakterien. Beitr Biol Pflanz Heft 2 1, 127–224 (in German). [Google Scholar]
  9. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E.(1977). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef] [Google Scholar]
  10. Combet-Blanc, Y., Ollivier, B., Streicher, C., Patel, B. K., Dwivedi, P. P., Pot, B., Prensier, G. & Garcia, J. L.(1995).Bacillus thermoamylovorans sp. nov., a moderately thermophilic and amylolytic bacterium. Int J Syst Bacteriol 45, 9–16.[CrossRef] [Google Scholar]
  11. Forsyth, G. & Logan, N. A.(2000). Isolation of Bacillus thuringiensis from northern Victoria Land, Antarctica. Lett Appl Microbiol 30, 263–266.[CrossRef] [Google Scholar]
  12. Ghosh, A., Bhardwaj, M., Satyanarayana, T., Khurana, M., Mayilraj, S. & Jain, R. K.(2007).Bacillus lehensis sp. nov., an alkalitolerant bacterium isolated from soil. Int J Syst Evol Microbiol 57, 238–242.[CrossRef] [Google Scholar]
  13. Gugliandolo, C., Maugeri, T. L., Caccamo, D. & Stackebrandt, E.(2003).Bacillus aeolius sp. nov. a novel thermophilic, halophilic marine Bacillus species from Eolian Islands (Italy). Syst Appl Microbiol 26, 172–176.[CrossRef] [Google Scholar]
  14. Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  15. Komagata, K. & Suzuki, K.(1987). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–206. [Google Scholar]
  16. Kumar, S., Tamura, K., Jakobsen, I.-B. & Nei, M.(2001).mega2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef] [Google Scholar]
  17. Lányí, B.(1987). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67. [Google Scholar]
  18. Larkin, J. M. & Stokes, J. L.(1967). Taxonomy of psychrophilic strains of Bacillus. J Bacteriol 94, 889–895. [Google Scholar]
  19. Lechner, S., Mayr, R., Francis, K. P., Prüß, B. M., Kaplan, T., Wießner-Gunkel, E., Stewart, G. S. & Scherer, S.(1998).Bacillus weihenstephanensis sp. nov. is a new psychrotolerant species of the Bacillus cereus group. Int J Syst Bacteriol 48, 1373–1382.[CrossRef] [Google Scholar]
  20. Lee, J. C., Lim, J. M., Park, D. J., Jeon, C. O., Li, W. J. & Kim, C. J.(2006).Bacillus seohaeanensis sp. nov., a halotolerant bacterium that contains l-lysine in its cell wall. Int J Syst Evol Microbiol 56, 1893–1898.[CrossRef] [Google Scholar]
  21. Lim, J. M., Jeon, C. O. & Kim, C. J.(2006).Bacillus taeanensis sp. nov., a halophilic Gram-positive bacterium from a solar saltern in Korea. Int J Syst Evol Microbiol 56, 2903–2908.[CrossRef] [Google Scholar]
  22. Logan, N. A., Lebbe, L., Hoste, B., Goris, J., Forsyth, G., Heyndrickx, M., Murray, B. L., Syme, N., Wynn-Williams, D. D. & De Vos, P.(2000). Aerobic endospore-forming bacteria from geothermal environments in northern Victoria Land, Antarctica, and Candlemas Island, South Sandwich archipelago, with the proposal of Bacillus fumarioli sp. nov. Int J Syst Evol Microbiol 50, 1741–1753. [Google Scholar]
  23. Nelson, L. M. & Parkinson, D.(1978). Growth characteristics of three bacterial isolates from an arctic soil. Can J Microbiol 24, 909–914.[CrossRef] [Google Scholar]
  24. Nogi, Y., Takami, H. & Horikoshi, K.(2005). Characterization of alkaliphilic Bacillus strains used in industry: proposal of five novel species. Int J Syst Evol Microbiol 55, 2309–2315.[CrossRef] [Google Scholar]
  25. Pandey, K. K., Mayilraj, S. & Chakrabarti, T.(2002).Pseudomonas indica sp. nov., a novel butane-utilizing species. Int J Syst Evol Microbiol 52, 1559–1567.[CrossRef] [Google Scholar]
  26. Priest, F. G., Goodfellow, M. & Todd, C.(1988). A numerical classification of the genus Bacillus. J Gen Microbiol 134, 1847–1882. [Google Scholar]
  27. Reddy, G. S. N., Aggarwal, R. K., Matsumoto, G. I. & Shivaji, S.(2000).Arthrobacter flavus sp. nov., a psychrophilic bacterium isolated from a pond in McMurdo Dry Valley, Antarctica. Int J Syst Evol Microbiol 50, 1553–1561.[CrossRef] [Google Scholar]
  28. Reddy, G. S. N., Prakash, J. S. S., Matsumoto, G. I., Stackebrandt, E. & Shivaji, S.(2002).Arthrobacter roseus sp. nov., a psychrophilic bacterium isolated from an Antarctic cyanobacterial mat sample. Int J Syst Evol Microbiol 52, 1017–1021.[CrossRef] [Google Scholar]
  29. Reddy, G. S. N., Nagy, M. & Garcia-Pichel, F.(2006).Belnapia moabensis gen. nov., sp. nov., an alphaproteobacterium from biological soil crusts in the Colorado Plateau, USA. Int J Syst Evol Microbiol 56, 51–58.[CrossRef] [Google Scholar]
  30. Rheims, H., Frühling, A., Schumann, P., Rohde, M. & Stackebrandt, E.(1999).Bacillus silvestris sp. nov., a new member of the genus Bacillus that contains lysine in its cell wall. Int J Syst Bacteriol 49, 795–802.[CrossRef] [Google Scholar]
  31. Rüger, H.-J., Fritze, D. & Spröer, C.(2000). New psychrophilic and psychrotolerant Bacillus marinus strains from tropical and polar deep-sea sediments and emended description of the species. Int J Syst Evol Microbiol 50, 1305–1313.[CrossRef] [Google Scholar]
  32. Shivaji, S., Chaturvedi, P., Suresh, K., Reddy, G. S. N., Dutt, C. B., Wainwright, M., Narlikar, J. V. & Bhargava, P. M.(2006).Bacillus aerius sp. nov., Bacillus aerophilus sp. nov., Bacillus stratosphericus sp. nov. and Bacillus altitudinis sp. nov., isolated from cryogenic tubes used for collecting air samples from high altitudes. Int J Syst Evol Microbiol 56, 1465–1473.[CrossRef] [Google Scholar]
  33. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  34. Spanka, R. & Fritze, D.(1993).Bacillus cohnii sp. nov., a new, obligately alkaliphilic, oval-spore-forming Bacillus species with ornithine and aspartic acid instead of diaminopimelic acid in the cell wall. Int J Syst Bacteriol 43, 150–156.[CrossRef] [Google Scholar]
  35. Suresh, K., Reddy, G. S. N., Sengupta, S. & Shivaji, S.(2004).Deinococcus indicus sp. nov., an arsenic-resistant bacterium from an aquifer in West Bengal, India. Int J Syst Evol Microbiol 54, 457–461.[CrossRef] [Google Scholar]
  36. Tamaoka, J.(1986). Analysis of bacterial menaquinone mixtures by reverse-phase high-performance liquid chromatography. Methods Enzymol 123, 251–256. [Google Scholar]
  37. Tamaoka, J., Katayama-Fujimura, Y. & Kuraishi, H.(1983). Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. J Appl Bacteriol 54, 31–36.[CrossRef] [Google Scholar]
  38. Thompson, J. D., Higgins, D. G. & Gibson, T. J.(1994).clustalw: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef] [Google Scholar]
  39. Vargas, V. A., Delgado, O. D., Hatti-Kaul, R. & Mattiasson, B.(2005).Bacillus bogoriensis sp. nov., a novel alkaliphilic, halotolerant bacterium isolated from a Kenyan soda lake. Int J Syst Evol Microbiol 55, 899–902.[CrossRef] [Google Scholar]
  40. Venkateswaran, K., Kempf, M., Chen, F., Satomi, M., Nicholson, W. & Kern, R.(2003).Bacillus nealsonii sp. nov., isolated from a spacecraft-assembly facility, whose spores are gamma-radiation resistant. Int J Syst Evol Microbiol 53, 165–172.[CrossRef] [Google Scholar]
  41. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  42. Yoon, J. H., Lee, K. C., Weiss, N., Kho, Y. H., Kang, K. H. & Park, Y. H.(2001).Sporosarcina aquimarina sp. nov., a bacterium isolated from seawater in Korea, and transfer of Bacillus globisporus (Larkin and Stokes 1967), Bacillus psychrophilus (Nakamura 1984) and Bacillus pasteurii (Chester 1898) to the genus Sporosarcina as Sporosarcina globispora comb. nov., Sporosarcina psychrophila comb. nov. and Sporosarcina pasteurii comb. nov., and emended description of the genus Sporosarcina. Int J Syst Evol Microbiol 51, 1079–1086.[CrossRef] [Google Scholar]
  43. Yumoto, I., Yamazaki, K., Sawabe, T., Nakano, K., Kawasaki, K., Ezura, Y. & Shinano, H.(1998).Bacillus horti sp. nov., a new Gram-negative alkaliphilic bacillus. Int J Syst Bacteriol 48, 565–571.[CrossRef] [Google Scholar]

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Transmission electron photomicrograph of a negatively stained cell of sp. nov. PN5 . Bar, 0.5 µm.


Phylogenetic trees based on 16S rRNA gene sequences (1478 bases) reconstructed using UPGMA (a), maximum evolution (b) and DNAPARS (c), showing the phylogenetic relationships between psychrotolerant species of . [PDF](23 KB)


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