1887

Abstract

Strain NJ-44, isolated from sediment of the eutrophic Guanting Reservoir in Beijing (China), was subjected to a taxonomic study using a polyphasic approach. The strain was aerobic, with salmon-pink-pigmented colonies on R2A agar. Cells were single, Gram-negative rods, motile by gliding. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain NJ-44 belonged to the phylum , with ATCC 13524 (90.8 % similarity), DY (90.5 %) and R2A15-11 (89.1 %) as its closest relatives. Strain NJ-44 was clearly differentiated from members of the genera and in its DNA G+C content (40.6 mol%) and its major fatty acids, iso-C G, iso-C, anteiso-C, iso-C 3-OH, iso-C 3-OH, anteiso-C A and iso-C 3-OH. It is proposed that strain NJ-44 represents a novel genus and species, named gen. nov., sp. nov. The type strain of is strain NJ-44 (=CGMCC 1.6845 =NBRC 103935).

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2008-09-01
2019-10-19
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References

  1. Bernardet, J.-F., Nakagawa, Y. & Holmes, B. ( 2002; ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52, 1049–1070.[CrossRef]
    [Google Scholar]
  2. Chun, J., Lee, J.-H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y. W. ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef]
    [Google Scholar]
  3. De Ley, J. ( 1970; ). Reexamination of the association between melting point, buoyant density, and chemical base composition of deoxyribonucleic acid. J Bacteriol 101, 738–754.
    [Google Scholar]
  4. Dong, X.-Z. & Cai, M.-Y. (editors) ( 2001; ). Determination of biochemical properties. In Manual for the Systematic Identification of General Bacteria, pp. 370–398. Beijing: Science Press (in Chinese).
  5. Garrity, G. M., Lilburn, T. G., Cole, J. R., Harrison, S. H., Euzéby, J. & Tindall, B. J. ( 2007; ). The Taxonomic Outline of Bacteria and Archaea, release 7.7, part 11, The Bacteria: Phyla Planctomycetes, Chlamydiae, Spirochaetes, Fibrobacteres, Acidobacteria, Bacteroidetes, Fusobacteria, Verrucomicrobia, Dictyoglomi, Gemmatomonadetes, and Lentisphaerae, pp. 540–595. http://www.taxonomicoutline.org/
  6. Johnson, J. L. ( 1985a; ). Determination of DNA base composition. Methods Microbiol 18, 1–31.
    [Google Scholar]
  7. Johnson, J. L. ( 1985b; ). DNA reassociation and RNA hybridisation of bacterial nucleic acids. Methods Microbiol 18, 33–74.
    [Google Scholar]
  8. Kämpfer, P., Young, C.-H., Sridhar, K. R., Arun, A. B., Lai, W. A., Shen, F. T. & Rekha, P. D. ( 2006; ). Transfer of [Flexibacter] sancti, [Flexibacter] filiformis, [Flexibacter] japonensis and [Cytophaga] arversicola to the genus Chitinophaga and description of Chitinophaga skermanii sp. nov. Int J Syst Evol Microbiol 56, 2223–2228.[CrossRef]
    [Google Scholar]
  9. Kim, B.-Y., Weon, H.-Y., Yoo, S.-H., Hong, S.-B., Kwon, S.-W., Stackebrandt, E. & Go, S.-J. ( 2007; ). Niabella aurantiaca gen. nov., sp. nov., isolated from a greenhouse soil in Korea. Int J Syst Evol Microbiol 57, 538–541.[CrossRef]
    [Google Scholar]
  10. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  11. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  12. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  13. Weon, H.-Y., Kim, B.-Y., Yoo, S.-H., Lee, S.-Y., Kwon, S.-W., Go, S.-J. & Stackebrandt, E. ( 2006; ). Niastella koreensis gen. nov., sp. nov. and Niastella yeongjuensis sp. nov., novel members of the phylum Bacteroidetes, isolated from soil cultivated with Korean ginseng. Int J Syst Evol Microbiol 56, 1777–1782.[CrossRef]
    [Google Scholar]
  14. Xie, C.-H. & Yokota, A. ( 2006; ). Reclassification of [Flavobacterium] ferrugineum as Terrimonas ferruginea gen. nov., comb. nov., and description of Terrimonas lutea sp. nov., isolated from soil. Int J Syst Evol Microbiol 56, 1117–1121.[CrossRef]
    [Google Scholar]
  15. Ying, J.-Y., Liu, Z.-P., Wang, B.-J., Dai, X., Yang, S.-S. & Liu, S.-J. ( 2007; ). Salegentibacter catena sp. nov., isolated from sediment of the South China Sea, and emended description of the genus Salegentibacter. Int J Syst Evol Microbiol 57, 219–222.[CrossRef]
    [Google Scholar]
  16. Zhu, F., Wang, S. & Zhou, P.-J. ( 2003; ). Flavobacterium xinjiangense sp. nov. and Flavobacterium omnivorum sp. nov., novel psychrophiles from the China No. 1 glacier. Int J Syst Evol Microbiol 53, 853–857.[CrossRef]
    [Google Scholar]
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vol. , part 9, pp. 2191 - 2194

Maximum-parsimony and minimum-evolution phylogenetic trees based on 16S rRNA gene sequences. [PDF](20 KB)



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