1887

Abstract

Two yeast strains (TM2-16 and PT1-17) were isolated by membrane filtration from samples of estuarine water collected from two mangrove forests, in Khao Lumpee-Haad Thaimueang National Park and Mu Ko Ra-Ko Prathong National Park, Phang-Nga Province, Thailand. Analysis of the D1/D2 domain of the large-subunit rDNA sequences revealed that the sequences of the two strains were identical. The closest species in terms of pairwise sequence similarity was , but the level of nucleotide substitutions (13.2 %) was sufficient to justify the description of a separate species. Phylogenetic analysis demonstrated that the two strains occupy a basal position with respect to and . of the clade, supported by a high bootstrap value. The two strains showed identical phenotypic characteristics, including proliferation by multilateral budding, absence of ascospores and ballistoconidia and negative Diazonium blue B and urease reactions. The major ubiquinone was Q-9. On the basis of the above findings, these two strains were assigned to a single novel species of the genus , for which the name sp. nov. is proposed. The type strain is PT1-17 (=BCC 21231 =NBRC 101967 =CBS 10407).

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2008-02-01
2019-10-17
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References

  1. Aksornkoae, S. ( 1999; ). Mangrove: Ecology and Management. Bangkok: Kasetsart University Press (in Thai).
  2. Altschul, S. F., Madden, T. L., Schäffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J. ( 1997; ). Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25, 3389–3402.[CrossRef]
    [Google Scholar]
  3. Fell, J. W., Statzell-Tallman, A. & Kurtzman, C. P. ( 2004; ). Lachancea meyersii sp. nov., an ascosporogenous yeast from mangrove regions in Bahama Islands. Stud Mycol 50, 359–363.
    [Google Scholar]
  4. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  5. Hagler, A. N. & Ahearn, D. G. ( 1987; ). Ecology of aquatic yeasts. In The Yeasts, 2nd edn, vol. 2, Yeasts and the Environment, pp. 181–205. Edited by A. H. Rose & J. S. Harrison. London: Academic Press.
  6. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  7. Kuraishi, H., Katayama-Fujimura, Y., Sugiyama, J. & Yokoyama, T. ( 1985; ). Ubiquinone systems in fungi. I. Distribution of ubiquinones in the major families of ascomycetes, basidiomycetes, and deuteromycetes, and their taxonomic implications. Trans Mycol Soc Jpn 26, 383–395.
    [Google Scholar]
  8. Kurtzman, C. P. ( 1998; ). Yarrowia van der Walt & von Arx. In The Yeasts, a Taxonomic Study, 4th edn, pp. 420–421. Edited by C. P. Kurtzman & J. W. Fell. Amsterdam: Elsevier.
  9. Kurtzman, C. P. ( 2005; ). New species and a new combination in the Hyphopichia and Yarrowia yeast clades. Antonie van Leeuwenhoek 88, 121–130.[CrossRef]
    [Google Scholar]
  10. Kurtzman, C. P. & Robnett, C. J. ( 1998; ). Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large-subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73, 331–371.[CrossRef]
    [Google Scholar]
  11. Lachance, M. A. & Starmer, W. T. ( 1998; ). Ecology and yeasts. In The Yeasts, a Taxonomic Study, 4th edn, pp. 21–30. Edited by C. P. Kurtzman & J. W. Fell. Amsterdam: Elsevier.
  12. Limtong, S., Yongmanitchai, W., Tun, M. M., Kawasaki, H. & Seki, T. ( 2007a; ). Kazachstania siamensis sp. nov., an ascomycetous yeast species from forest soil in Thailand. Int J Syst Evol Microbiol 57, 419–422.[CrossRef]
    [Google Scholar]
  13. Limtong, S., Yongmanitchai, W., Kawasaki, H. & Seki, T. ( 2007b; ). Candida thaimueangensis sp. nov., an anamorphic yeast species from estuarine water in a mangrove forest in Thailand. Int J Syst Evol Microbiol 57, 650–653.[CrossRef]
    [Google Scholar]
  14. Nakase, T. & Suzuki, M. ( 1986; ). Bullera megalospora, a new species of yeast forming larger ballistospores isolated from dead leaves of Oryza sativa, Miscanthus sinensis and Sasa sp. in Japan. J Gen Appl Microbiol 32, 225–240.[CrossRef]
    [Google Scholar]
  15. Péter, G., Dlauchy, D., Vasdinyei, R., Tornai-Lehoczki, J. & Deák, T. ( 2004; ). Candida galli sp. nov., a new yeast from poultry. Antonie van Leeuwenhoek 86, 105–110.[CrossRef]
    [Google Scholar]
  16. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  17. Smith, M. Th. ( 1998; ). Aciculoconidium D. S. King & S. C. Jong. In The Yeasts, a Taxonomic Study, 4th edn, pp. 439–440. Edited by C. P. Kurtzman & J. W. Fell. Amsterdam: Elsevier.
  18. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  19. Yamada, Y. & Kondo, K. ( 1973; ). Coenzyme Q system in the classification of the yeast genera Rhodotorula and Cryptococcus and the yeast like genera Sporobolomyces and Rhodosporidium. J Gen Appl Microbiol 19, 59–77.[CrossRef]
    [Google Scholar]
  20. Yarrow, D. ( 1998; ). Methods for the isolation, maintenance and identification of yeasts. In The Yeasts, a Taxonomic Study, 4th edn, pp. 77–100. Edited by C. P. Kurtzman & J. W. Fell. Amsterdam: Elsevier.
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