1887

Abstract

A yellow-pigmented, scotochromogenic, slowly growing mycobacterial strain, designated T1921, was isolated from the disseminated osteomyelitic lesions of a 7-year-old child with an underlying partial gamma interferon receptor alpha-1 deficiency. Hybridization by the line probe assay indicated the presence of a species. Sequencing of the 16S rRNA gene, the internally transcribed spacer (ITS) region and the and genes revealed that strain T1921 could be differentiated from all recognized species of the genus . Phylogenetic analysis based on the 16S rRNA gene indicated that strain T1921 was related most closely to , whereas analysis based on the ITS and and genes indicated that it was most closely related to . Phenotypic tests were not able to differentiate strain T1921 from similar slowly growing mycobacteria. Strain T1921 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is T1921 (=DSM 45069 =ATCC BAA-1401).

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2008-10-01
2019-10-14
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References

  1. Adekambi, T., Colson, P. & Drancourt, M. ( 2003; ). rpoB-based identification of nonpigmented and late-pigmenting rapidly growing mycobacteria. J Clin Microbiol 41, 5699–5708.[CrossRef]
    [Google Scholar]
  2. American Thoracic Society ( 1997; ). Diagnosis and treatment of disease caused by nontuberculous mycobacteria. Am J Respir Crit Care Med 156, S1–S25.[CrossRef]
    [Google Scholar]
  3. Arend, S. M., Janssen, R., Gosen, J. J., Waanders, H., de Boer, T., Ottenhoff, T. H. & van Dissel, J. T. ( 2001; ). Multifocal osteomyelitis caused by nontuberculous mycobacteria in patients with a genetic defect of the interferon-gamma receptor. Neth J Med 59, 140–151.[CrossRef]
    [Google Scholar]
  4. Butler, W. R., Floyd, M. M., Silcox, V., Cage, G., Desmond, E., Duffey, P. S., Guthertz, L. S., Gross, W. M., Jost, K. C. & other authors ( 1996; ). Standardized Method for HPLC Identification of Mycobacteria. Atlanta, GA: US Department of Public Health Service Centers for Disease Control and Prevention.
  5. Collert, S., Petrini, B. & Wickman, K. ( 1983; ). Osteomyelitis caused by Mycobacterium avium. Acta Orthop Scand 54, 449–451.[CrossRef]
    [Google Scholar]
  6. Heifets, L. ( 2004; ). Mycobacterial infections caused by nontuberculous mycobacteria. Semin Respir Crit Care Med 25, 283–295.[CrossRef]
    [Google Scholar]
  7. Herlin, T., Thelle, T., Kragballe, K., Borregaard, N. & Thestrup-Pedersen, K. ( 1981; ). Sustained depression of monocyte cytotoxicity in a boy with disseminated nontuberculous mycobacteriosis. J Pediatr 99, 264–267.[CrossRef]
    [Google Scholar]
  8. Janda, J. M. & Abbott, S. L. ( 2002; ). Bacterial identification for publication: when is enough enough? J Clin Microbiol 40, 1887–1891.[CrossRef]
    [Google Scholar]
  9. Kirschner, P., Springer, B., Vogel, U., Meier, A., Wrede, A., Kiekenbeck, M., Bange, F. C. & Böttger, E. C. ( 1993; ). Genotypic identification of mycobacteria by nucleic acid sequence determination: report of a 2-year experience in a clinical laboratory. J Clin Microbiol 31, 2882–2889.
    [Google Scholar]
  10. Lévy-Frébault, V. V. & Portaels, F. ( 1992; ). Proposed minimal standards for the genus Mycobacterium and for description of new slowly growing Mycobacterium species. Int J Syst Bacteriol 42, 315–323.[CrossRef]
    [Google Scholar]
  11. McNabb, A., Eisler, D., Adie, K., Amos, M., Rodrigues, M., Stephens, G., Black, W. A. & Isaac-Renton, J. ( 2004; ). Assessment of partial sequencing of the 65-kilodalton heat shock protein gene (hsp65) for routine identification of Mycobacterium species isolated from clinical sources. J Clin Microbiol 42, 3000–3011.[CrossRef]
    [Google Scholar]
  12. Mijs, W., de Haas, P., Rossau, R., Van der Laan, T., Rigouts, L., Portaels, F. & van Soolingen, D. ( 2002; ). Molecular evidence to support a proposal to reserve the designation Mycobacterium avium subsp. avium for bird-type isolates and ‘M. avium subsp. hominissuis’ for the human/porcine type of M. avium. Int J Syst Evol Microbiol 52, 1505–1518.[CrossRef]
    [Google Scholar]
  13. Murcia, M. I., Tortoli, E., Menendez, M. C., Palenque, E. & Garcia, M. J. ( 2006; ). Mycobacterium colombiense sp. nov., a novel member of the Mycobacterium avium complex and description of MAC-X as a new ITS genetic variant. Int J Syst Evol Microbiol 56, 2049–2054.[CrossRef]
    [Google Scholar]
  14. Murray, P. R., Baron, E. J., Jorgensen, J. H., Landry, M. L. & Pfaller, M. A. ( 2007; ). Manual of Clinical Microbiology, 9th edn. Washington, DC: American Society for Microbiology.
  15. Raszka, W. V., Jr, Trinh, T. T. & Zawadsky, P. M. ( 1994; ). Multifocal M. intracellulare osteomyelitis in an immunocompetent child. Clin Pediatr (Phila) 33, 611–616.[CrossRef]
    [Google Scholar]
  16. Roth, A., Fischer, M., Hamid, M. E., Michalke, S., Ludwig, W. & Mauch, H. ( 1998; ). Differentiation of phylogenetically related slowly growing mycobacteria based on 16S–23S rRNA gene internal transcribed spacer sequences. J Clin Microbiol 36, 139–147.
    [Google Scholar]
  17. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  18. Siddiqi, S. H. ( 1996; ). BACTEC 460 TB SYSTEM. Product and Procedure Manual. Sparks, MD: Becton Dickinson Microbiology Systems.
  19. Smithwick, R. W. ( 1979; ). Laboratory Manual for Acid-Fast Microscopy, 2nd edn. Atlanta, GA: US Public Health Service.
  20. Storgaard, M., Varming, K., Herlin, T. & Obel, N. ( 2006; ). Novel mutation in the interferon-gamma-receptor gene and susceptibility to mycobacterial infections. Scand J Immunol 64, 137–139.[CrossRef]
    [Google Scholar]
  21. Torkko, P., Suutari, M., Suomalainen, S., Paulin, L., Larsson, L. & Katila, M. L. ( 1998; ). Separation among species of Mycobacterium terrae complex by lipid analyses: comparison with biochemical tests and 16S rRNA sequencing. J Clin Microbiol 36, 499–505.
    [Google Scholar]
  22. Torkko, P., Katila, M. L. & Kontro, M. ( 2003; ). Gas-chromatographic lipid profiles in identification of currently known slowly growing environmental mycobacteria. J Med Microbiol 52, 315–323.[CrossRef]
    [Google Scholar]
  23. Tortoli, E., Bartoloni, A., Böttger, E. C., Emler, S., Garzelli, C., Magliano, E., Mantella, A., Rastogi, N., Rindi, L. & other authors ( 2001; ). Burden of unidentifiable mycobacteria in a reference laboratory. J Clin Microbiol 39, 4058–4065.[CrossRef]
    [Google Scholar]
  24. Tortoli, E., Rindi, L., Garcia, M. J., Chiaradonna, P., Dei, R., Garzelli, C., Kroppenstedt, R. M., Lari, N., Mattei, R. & other authors ( 2004; ). Proposal to elevate the genetic variant MAC-A, included in the Mycobacterium avium complex, to species rank as Mycobacterium chimaera sp. nov. Int J Syst Evol Microbiol 54, 1277–1285.[CrossRef]
    [Google Scholar]
  25. Villella, A., Picard, C., Jouanguy, E., Dupuis, S., Popko, S., Abughali, N., Meyerson, H., Casanova, J. L. & Hostoffer, R. W. ( 2001; ). Recurrent Mycobacterium avium osteomyelitis associated with a novel dominant interferon gamma receptor mutation. Pediatrics 107, E47 [CrossRef]
    [Google Scholar]
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vol. , part 10, pp. 2398 - 2402

Neighbour-joining phylogenetic trees based on the and gene sequences of strain T1921 compared with selected mycobacterial species. [PDF](20 KB)



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