An aerobic, motile, Gram-negative, ice-active substance-producing, rod-shaped psychrophile, designated strain ArB 0140, was isolated from seawater collected from near a glacier in Kongsfjorden, Svalbard Archipelago, Norway. Phylogenetic analysis using 16S rRNA gene sequences indicated that strain ArB 0140 showed a distinct phyletic line within the genus . Characteristic chemotaxonomic data [predominant isoprenoid quinone, Q8; major fatty acids, C, C, C, C and C (docosahexaenoic acid; DHA)] also corroborated the affiliation of strain ArB 0140 to the genus . The maximal growth rate of the novel strain was observed at 9 °C, with a maximum temperature for growth of 18 °C. The genomic DNA G+C content was 46.9 mol%. Based on the data obtained from this polyphasic study, including DNA–DNA relatedness, physiological and biochemical tests and ice-controlling activity, strain ArB 0140 was found to be genetically and phenotypically different from other recognized species of the genus . Therefore strain ArB 0140 represents a novel species, for which the name sp. nov. is proposed. The type strain is ArB 0140 (=KCTC 10814=KCCM 42845=JCM 14759).


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vol. , part 4, pp. 817 - 820

A scanning electron micrograph of cells of strain ArB 0140 .

A graph showing the growth rate of strain ArB 0140 over a range of temperatures.

Images showing the ice-modifiying activity of strain ArB 0140 and other species of the genus .

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