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An aerobic, motile, Gram-negative, ice-active substance-producing, rod-shaped psychrophile, designated strain ArB 0140T, was isolated from seawater collected from near a glacier in Kongsfjorden, Svalbard Archipelago, Norway. Phylogenetic analysis using 16S rRNA gene sequences indicated that strain ArB 0140T showed a distinct phyletic line within the genus Moritella. Characteristic chemotaxonomic data [predominant isoprenoid quinone, Q8; major fatty acids, C14 : 0, C14 : 1, C16 : 0, C16 : 1 and C22 : 6 (docosahexaenoic acid; DHA)] also corroborated the affiliation of strain ArB 0140T to the genus Moritella. The maximal growth rate of the novel strain was observed at 9 °C, with a maximum temperature for growth of 18 °C. The genomic DNA G+C content was 46.9 mol%. Based on the data obtained from this polyphasic study, including DNA–DNA relatedness, physiological and biochemical tests and ice-controlling activity, strain ArB 0140T was found to be genetically and phenotypically different from other recognized species of the genus Moritella. Therefore strain ArB 0140T represents a novel species, for which the name Moritella dasanensis sp. nov. is proposed. The type strain is ArB 0140T (=KCTC 10814T=KCCM 42845T=JCM 14759T).
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International Journal of Systematic and Evolutionary Microbiology vol. 58 , part 4, pp. 817 - 820
Supplementary Fig. S1. A scanning electron micrograph of cells of strain ArB 0140 T.
Supplementary Fig. S2. A graph showing the growth rate of strain ArB 0140 Tover a range of temperatures.
Supplementary Fig. S3. Images showing the ice-modifiying activity of strain ArB 0140 Tand other species of the genus Moritella .
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