@article{mbs:/content/journal/ijsem/10.1099/ijs.0.65499-0, author = "Rivas, Raúl and García-Fraile, Paula and Zurdo-Piñeiro, José Luis and Mateos, Pedro F. and Martínez-Molina, Eustoquio and Bedmar, Eulogio J. and Sánchez-Raya, Juan and Velázquez, Encarna", title = "Saccharibacillus sacchari gen. nov., sp. nov., isolated from sugar cane", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2008", volume = "58", number = "8", pages = "1850-1854", doi = "https://doi.org/10.1099/ijs.0.65499-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.65499-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A bacterial strain designated GR21T was isolated from apoplastic fluid of Saccharum officinarum (sugar cane). Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate forms a separate branch within the family ‘Paenibacillaceae’, with Paenibacillus as the closest related genus. Within this genus, the closest related species is Paenibacillus xylanilyticus, with 93.4 % similarity to the sequence of the type strain. The isolate has Gram-variable, facultatively anaerobic, rod-shaped cells, motile by polar and subpolar flagella. Round, non-ornamented, central or subterminal spores are formed in unswollen sporangia. The strain is catalase-positive and oxidase-negative on nutrient agar medium. Cellulose and aesculin were hydrolysed, whereas xylan, starch and gelatin were not. Growth was supported by many carbohydrates as carbon sources. Strain GR21T displayed a lipid profile consisting of diphosphatidylglycerol, phosphatidylglycerol, an unknown aminophospholipid, two unknown glycolipids and an unknown phosphoglycolipid. MK-7 was the predominant menaquinone and anteiso-C15 : 0 was the major fatty acid. The DNA G+C content was 57.8 mol%. Phylogenetic and phenotypic analyses, including assimilation of carbon sources and exoenzyme production commonly used for classification within the family ‘Paenibacillaceae’, showed that strain GR21T belongs to a new genus within this family, for which the name Saccharibacillus sacchari gen. nov., sp. nov. is proposed. The type strain of Saccharibacillus sacchari is GR21T (=LMG 24085T =DSM 19268T).", }