A bacterial strain designated GR21 was isolated from apoplastic fluid of (sugar cane). Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate forms a separate branch within the family ‘’, with as the closest related genus. Within this genus, the closest related species is , with 93.4 % similarity to the sequence of the type strain. The isolate has Gram-variable, facultatively anaerobic, rod-shaped cells, motile by polar and subpolar flagella. Round, non-ornamented, central or subterminal spores are formed in unswollen sporangia. The strain is catalase-positive and oxidase-negative on nutrient agar medium. Cellulose and aesculin were hydrolysed, whereas xylan, starch and gelatin were not. Growth was supported by many carbohydrates as carbon sources. Strain GR21 displayed a lipid profile consisting of diphosphatidylglycerol, phosphatidylglycerol, an unknown aminophospholipid, two unknown glycolipids and an unknown phosphoglycolipid. MK-7 was the predominant menaquinone and anteiso-C was the major fatty acid. The DNA G+C content was 57.8 mol%. Phylogenetic and phenotypic analyses, including assimilation of carbon sources and exoenzyme production commonly used for classification within the family ‘’, showed that strain GR21 belongs to a new genus within this family, for which the name gen. nov., sp. nov. is proposed. The type strain of is GR21 (=LMG 24085 =DSM 19268).


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Electron micrographs of cells of strain GR21 grown on nutrient agar for 48 h (a), showing the polar and subpolar flagella, and for 72 h (b, c), showing the round spores. Insertion of flagella is marked by arrows. Bars, 1 mm (a, b) and 2 mm (c).




Extended comparative sequence analysis of 16S rRNA genes from GR21 ( gen. nov., sp. nov.) and representative species of related genera from the family ' ' using the neighbour-joining method. [ PDF] 66 KB


Two-dimensional thin-layer chromatogram of polar lipids of strain GR21 . DPG, Diphosphatidylglycerol; PG, phosphatidylglycerol; PN, unknown aminophospholipid; GL1 and GL2, unknown glycolipids; PGL, unknown phosphoglycolipid.

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