1887

Abstract

Strain SC-087B, isolated from sewage sludge compost during a study of bacterial diversity in composts, was characterized. The isolate was a Gram-positive, short rod that was motile, catalase- and oxidase-negative and able to grow at 27–45 °C, pH 5.5–9.7 and in up to 10 % NaCl. The peptidoglycan was of the B2 type, containing the characteristic amino acids ornithine, homoserine and hydroxyglutamic acid. The muramic acid residues of the peptidoglycan were partially glycolylated. The major cell-wall sugar was mannose; traces of xylose were also detected. The predominant fatty acids, comprising more than 70 % of the total, were anteiso-C and anteiso-C, the major respiratory quinone was menaquinone-12 (MK-12) and the G+C content of the genomic DNA was 72 mol%. Based on analysis of the 16S rRNA gene sequence, the closest phylogenetic neighbours of strain SC-087B were members of the family , showing sequence similarity values of around 96 % with members of the species (96.0 %), (95.6 %) and (95.7 %). The chemotaxonomic and phenotypic traits analysed supported the inclusion of this strain within the genus and the proposal of a novel species. The name sp. nov. is proposed and the type strain is SC-087B (=DSM 19459=CCUG 54537).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65494-0
2008-07-01
2020-01-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/7/1700.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65494-0&mimeType=html&fmt=ahah

References

  1. Behrendt, U., Ulrich, A. & Schumann, P. ( 2001; ). Description of Microbacterium foliorum sp. nov. and Microbacterium phyllosphaerae sp. nov., isolated from the phyllosphere of grasses and the surface litter after mulching the sward, and reclassification of Aureobacterium resistens (Funke et al. 1998) as Microbacterium resistens comb. nov. Int J Syst Evol Microbiol 51, 1267–1276.
    [Google Scholar]
  2. Brennan, N. M., Brown, R., Goodfellow, M., Ward, A. C., Beresford, T. P., Vancanneyt, M., Cogan, T. M. & Fox, P. F. ( 2001; ). Microbacterium gubbeenense sp. nov., from the surface of a smear-ripened cheese. Int J Syst Evol Microbiol 51, 1969–1976.[CrossRef]
    [Google Scholar]
  3. Epstein, E. ( 1997; ). The Science of Composting. Lancaster, PA: Technomic Publishing.
  4. Euzéby, J. P. ( 1997; ). List of Bacterial Names with Standing in Nomenclature: a folder available on the Internet. Int J Syst Bacteriol 47, 590–592. (List of Prokaryotic Names with Standing in Nomenclature. Last full update May 02, 2008 URL: http://www.bacterio.net).[CrossRef]
    [Google Scholar]
  5. Ferreira da Silva, M., Tiago, I., Veríssimo, A., Boaventura, R. A. R., Nunes, O. C. & Manaia, C. M. ( 2006; ). Antibiotic resistance of enterococci and related bacteria in an urban wastewater treatment plant. FEMS Microbiol Ecol 55, 322–329.[CrossRef]
    [Google Scholar]
  6. Ferreira da Silva, M., Vaz-Moreira, I., Gonzalez-Pajuelo, M., Nunes, O. C. & Manaia, C. M. ( 2007; ). Antimicrobial resistance patterns in Enterobacteriaceae isolated from an urban wastewater treatment plant. FEMS Microbiol Ecol 60, 166–176.[CrossRef]
    [Google Scholar]
  7. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  8. Komagata, K. & Suzuki, K.-I. ( 1984; ). Genus Aureobacterium Collins, Jones, Keddie, Kroppenstedt and Schleifer 1983, 672VP. In Bergey's Manual of Systematic Bacteriology, vol. 2, pp. 1323–1325. Edited by J. T. Staley, M. P. Bryant, N. Pfennig & J. G. Holt. Baltimore: Williams & Wilkins.
  9. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  10. MacKenzie, S. L. ( 1987; ). Gas chromatographic analysis of amino acids as the N-heptafluorobutyryl isobutyl esters. J Assoc Off Anal Chem 70, 151–160.
    [Google Scholar]
  11. Matsuyama, H., Kawasaki, K., Yumoto, I. & Shida, O. ( 1999; ). Microbacterium kitamiense sp. nov., a new polysaccharide-producing bacterium isolated from the wastewater of a sugar-beet factory. Int J Syst Bacteriol 49, 1353–1357.[CrossRef]
    [Google Scholar]
  12. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  13. Murray, R. G. E., Doetsch, R. N. & Robinow, F. ( 1994; ). Determinative and cytological light microscopy. In Methods for General and Molecular Bacteriology, pp. 21–41. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  14. Rhuland, L. E., Work, E., Denman, R. F. & Hoare, D. S. ( 1955; ). The behavior of the isomers of α,ϵ-diaminopimelic acid on paper chromatograms. J Am Chem Soc 77, 4844–4846.[CrossRef]
    [Google Scholar]
  15. Richert, K., Brambilla, E. & Stackebrandt, E. ( 2007; ). The phylogenetic significance of peptidoglycan types: molecular analysis of the genera Microbacterium and Aureobacterium based upon sequence comparison of gyrB, rpoB, recA and ppk and 16S rRNA genes. Syst Appl Microbiol 30, 102–108.[CrossRef]
    [Google Scholar]
  16. Schippers, A., Bosecker, K., Spröer, C. & Schumann, P. ( 2005; ). Microbacterium oleivorans sp. nov. and Microbacterium hydrocarbonoxydans sp. nov., novel crude-oil-degrading Gram-positive bacteria. Int J Syst Evol Microbiol 55, 655–660.[CrossRef]
    [Google Scholar]
  17. Schleifer, K. H. & Seidl, P. H. ( 1985; ). Chemical composition and structure of murein. In Chemical Methods in Bacterial Systematics, pp. 201–219. Edited by M. Goodfellow & D. E. Minnikin. London: Academic Press.
  18. Schumann, P., Prauser, H., Rainey, F. A., Stackebrandt, E. & Hirsch, P. ( 1997; ). Friedmanniella antarctica gen. nov., sp. nov., an ll-diaminopimelic acid-containing actinomycete from Antarctic sandstone. Int J Syst Bacteriol 47, 278–283.[CrossRef]
    [Google Scholar]
  19. Shivaji, S., Bhadra, B., Rao, R. S., Chaturvedi, P., Pindi, P. K. & Raghukuma, C. ( 2007; ). Microbacterium indicum sp. nov., isolated from a deep-sea sediment sample from the Chagos Trench, Indian Ocean. Int J Syst Evol Microbiol 57, 1819–1822.[CrossRef]
    [Google Scholar]
  20. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  21. Staneck, J. L. & Roberts, G. D. ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226–231.
    [Google Scholar]
  22. Takeuchi, M. & Hatano, K. ( 1998a; ). Union of the genera Microbacterium Orla-Jensen and Aureobacterium Collins et al. in a redefined genus Microbacterium. Int J Syst Bacteriol 48, 739–747.[CrossRef]
    [Google Scholar]
  23. Takeuchi, M. & Hatano, K. ( 1998b; ). Proposal of six new species in the genus Microbacterium and transfer of Flavobacterium marinotypicum ZoBell and Upham to the genus Microbacterium as Microbacterium maritypicum comb. nov. Int J Syst Bacteriol 48, 973–982.[CrossRef]
    [Google Scholar]
  24. Tiago, I., Teixeira, I., Silva, S., Chung, P., Veríssimo, A. & Manaia, C. M. ( 2004; ). Metabolic and genetic diversity of mesophilic and thermophilic bacteria isolated from composted municipal sludge on poly-ϵ-caprolactones. Curr Microbiol 49, 407–414.[CrossRef]
    [Google Scholar]
  25. Tindall, B. J. ( 1989; ). Fully saturated menaquinones in the archaebacterium Pyrobaculum islandicum. FEMS Microbiol Lett 60, 251–254.[CrossRef]
    [Google Scholar]
  26. Uchida, K., Kudo, T., Suzuki, K. & Nakase, T. ( 1999; ). A new rapid method of glycolate test by diethyl ether extraction, which is applicable to a small amount of bacterial cells of less than one milligram. J Gen Appl Microbiol 45, 49–56.[CrossRef]
    [Google Scholar]
  27. Vaz-Moreira, I., Nobre, M. F., Nunes, O. C. & Manaia, C. M. ( 2007; ). Gulbenkiania mobilis gen. nov., sp. nov., isolated from treated municipal wastewater. Int J Syst Evol Microbiol 57, 1108–1112.[CrossRef]
    [Google Scholar]
  28. Vaz-Moreira, I., Silva, M. E., Manaia, C. M. & Nunes, O. C. ( 2008; ). Diversity of bacterial isolates from commercial and homemade composts. Microb Ecol 55, 714–722.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65494-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65494-0
Loading

Data & Media loading...

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error