%0 Journal Article %A Shiratori, Hatsumi %A Ohiwa, Hitomi %A Ikeno, Hironori %A Ayame, Shohei %A Kataoka, Naoaki %A Miya, Akiko %A Beppu, Teruhiko %A Ueda, Kenji %T Lutispora thermophila gen. nov., sp. nov., a thermophilic, spore-forming bacterium isolated from a thermophilic methanogenic bioreactor digesting municipal solid wastes %D 2008 %J International Journal of Systematic and Evolutionary Microbiology, %V 58 %N 4 %P 964-969 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.65490-0 %K DMA, dimethyl acetal %I Microbiology Society, %X A novel anaerobic, moderately thermophilic, spore-forming, rod-shaped bacterium (strain EBR46T) was isolated from an enrichment culture derived from an anaerobic thermophilic (55 °C) methanogenic bioreactor treating artificial solid wastes. Phylogeny based on 16S rRNA gene sequence analysis placed strain EBR46T within a distinct lineage between Clostridium clusters II and III. The closest recognized relative of strain EBR46T was Gracilibacter thermotolerans DSM 17427T (85.3 % 16S rRNA gene sequence similarity). The DNA G+C content of strain EBR46T was 36.2 mol%. The novel strain grew optimally at 55–58 °C and at pH 7.5–8.0 and was able to grow on peptone, tryptone, Casamino acids, casein hydrolysate, methionine, threonine, tryptophan, cysteine, lysine and serine in the presence of 0.2 % yeast extract. Carbohydrates were not utilized. The main products from tryptone utilization were acetate, iso-butyrate, propionate and iso-valerate. Strain EBR46T produced hydrogen sulfide from cysteine. The major fatty acids were iso-C15 : 0, C14 : 0, C16 : 0 DMA (dimethyl acetal) and iso-C15 : 0 DMA. Based on its unique phylogenetic and physiological features, strain EBR46T is considered to represent a novel species of a new genus, for which the name Lutispora thermophila gen. nov., sp. nov. is proposed. The type strain of the type species is EBR46T (=NBRC 102133T=DSM 19022T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.65490-0