1887

Abstract

A novel anaerobic, moderately thermophilic, spore-forming, rod-shaped bacterium (strain EBR46) was isolated from an enrichment culture derived from an anaerobic thermophilic (55 °C) methanogenic bioreactor treating artificial solid wastes. Phylogeny based on 16S rRNA gene sequence analysis placed strain EBR46 within a distinct lineage between clusters II and III. The closest recognized relative of strain EBR46 was DSM 17427 (85.3 % 16S rRNA gene sequence similarity). The DNA G+C content of strain EBR46 was 36.2 mol%. The novel strain grew optimally at 55–58 °C and at pH 7.5–8.0 and was able to grow on peptone, tryptone, Casamino acids, casein hydrolysate, methionine, threonine, tryptophan, cysteine, lysine and serine in the presence of 0.2 % yeast extract. Carbohydrates were not utilized. The main products from tryptone utilization were acetate, iso-butyrate, propionate and iso-valerate. Strain EBR46 produced hydrogen sulfide from cysteine. The major fatty acids were iso-C, C, C DMA (dimethyl acetal) and iso-C DMA. Based on its unique phylogenetic and physiological features, strain EBR46 is considered to represent a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is EBR46 (=NBRC 102133=DSM 19022).

Keyword(s): DMA, dimethyl acetal
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2008-04-01
2019-10-17
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