A Gram-negative, non-spore-forming bacterium, designated strain BD-d46T, was isolated from a playground soil sample in Jinju, South Korea. Cells were straight or curved rods and showed catalase- and oxidase-positive reactions. Growth of strain BD-d46T was observed between 15 and 35 °C (optimum 25–30 °C) and between pH 6.5 and 8.0 (optimum pH 7.0–7.5). The predominant fatty acids were summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH), C12 : 0 3-OH and C16 : 0. Strain BD-d46T contained phosphatidylethanolamine and phosphatidylglycerol as major polar lipids. Isoprenoid quinones were Q-8 (75 %) and MK-7 (25 %). The G+C content of the genomic DNA was 49.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BD-d46T formed a distinct lineage with Rheinheimera chironomi K19414T within the genus Rheinheimera. Levels of 16S rRNA gene sequence similarity between strain BD-d46T and the type strains of recognized Rheinheimera species ranged from 94.4 to 96.9 %. On the basis of chemotaxonomic data and molecular properties, strain BD-d46T is considered to represent a novel species of the genus Rheinheimera, for which the name Rheinheimera soli sp. nov. is proposed. The type strain is BD-d46T (=KCTC 22077T =DSM 19413T).
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Supplementary Fig. S1. Transmission electron micrograph
showing the general morphology of a negatively stained cell of
strain BD-c46
Tafter growth for 3 days at 30 °C in R2A
broth. Bar, 1 µm.
Supplementary Fig. S2. Polar lipid thin-layer
chromatograms sprayed with molybdatophosphoric acid reagent of
strain BD-d46
T(A) and
R. chironomi K19414
T(B). Ascending solvent systems: (I)
chloroform/methanol/water (65:25:4, by vol.); (II)
chloroform/acetic acid/methanol/water (80:13:10:2, by vol.).
For detection of polar lipids, molybdatophosphoric acid (PE,
PG, PL1, AL1, AL2 and AL3), ninhydrin (PE, AL1, AL2 and AL3)
and molybdenum blue reagent (PG and PE) were applied. PE,
Phosphatidylethanolamine; PG, phosphatidylglycerol; PL1,
unknown polar lipid; AL1, AL2 and AL3, unknown polar lipids
containing amino groups.
Supplementary Table S1. Cellular fatty acid compositions
of strain BD-d46
Tcultivated on MA and TSA.
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