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Abstract

A Gram-negative, strictly aerobic, non-spore-forming bacterium, designated strain BD-c194, was isolated from diesel-contaminated soil in Geoje, Korea. The cells were short, motile rods with single polar flagella. Growth of strain BD-c194 was observed between 15 and 45 °C (optimally at 30–35 °C) and between pH 6.0 and 9.5 (optimally at pH 7.5–9.0). The predominant fatty acids were 11-methyl C , C, C , C and an unknown fatty acid (equivalent chain-length 18.814); a large amount of phosphatidylglycerol and a small amount of diphosphatidylglycerol were present as polar lipids. The G+C content of the genomic DNA was 60.8 mol% and the major isoprenoid quinone was Q-10. A comparative 16S rRNA gene sequence analysis showed that strain BD-c194 formed a well-defined phyletic lineage within the genus (with 100 % bootstrap support). The levels of 16S rRNA gene sequence similarity with respect to the type strains of other species ranged from 95.0 to 96.1 %. On the basis of chemotaxonomic data and molecular properties, strain BD-c194 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is BD-c194 (=KCTC 22082 =DSM 19414).

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2008-03-01
2021-10-18
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References

  1. Cole, J. R., Chai, B., Marsh, T. L., Farris, R. J., Wang, Q., Kulam, S. A., Chandra, S., McGarrell, D. M., Schmidt, T. M. & other authors(2003). The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy. Nucleic Acids Res 31, 442–443.[CrossRef] [Google Scholar]
  2. Felsenstein, J.(2002).phylip (phylogeny inference package), version 3.6a. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  3. Foster, J. W.(1944). Microbiological aspects of riboflavin. I. Introduction. II. Bacterial oxidation of riboflavin to lumochrome. J Bacteriol 47, 27–41. [Google Scholar]
  4. Gomori, G.(1955). Preparation of buffers for use in enzyme studies. Methods Enzymol 1, 138–146. [Google Scholar]
  5. Jeon, C. O., Lim, J. M., Lee, J. M., Xu, L. H., Jiang, C. L. & Kim, C. J.(2005). Reclassification of Bacillus haloalkaliphilus Fritze 1996 as Alkalibacillus haloalkaliphilus gen. nov., comb. nov. and the description of Alkalibacillus salilacus sp. nov., a novel halophilic bacterium isolated from a salt lake in China. Int J Syst Evol Microbiol 55, 1891–1896.[CrossRef] [Google Scholar]
  6. Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  7. Komagata, K. & Suzuki, K.(1987). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207. [Google Scholar]
  8. Lane, D. J.(1991). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. Chichester: Wiley.
  9. Lanyi, B.(1987). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67. [Google Scholar]
  10. Lee, S. D.(2007).Devosia subaequoris sp. nov., isolated from beach sediment. Int J Syst Evol Microbiol 57, 2212–2215.[CrossRef] [Google Scholar]
  11. Leifson, E.(1963). Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85, 1183–1184. [Google Scholar]
  12. Nakagawa, Y., Sakane, T. & Yokota, A.(1996). Transfer of “Pseudomonas riboflavina” (Foster 1944), a gram-negative, motile rod with long-chain 3-hydroxy fatty acids, to Devosia riboflavina gen. nov., sp. nov., nom. rev. Int J Syst Bacteriol 46, 16–22.[CrossRef] [Google Scholar]
  13. Rivas, R., Willems, A., Subba-Rao, N. S., Mateos, P. F., Dazzo, F. B., Kroppenstedt, R. M., Martínez-Molina, E., Gillis, M. & Velázquez, E.(2003). Description of Devosia neptuniae sp. nov. that nodulates and fixes nitrogen in symbiosis with Neptunia natans, an aquatic legume from India. Syst Appl Microbiol 26, 47–53.[CrossRef] [Google Scholar]
  14. Rosselló-Mora, R. & Amann, R.(2001). The species concept for prokaryotes. FEMS Microbiol Rev 25, 39–67.[CrossRef] [Google Scholar]
  15. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  16. Stackebrandt, E., Frederiksen, W., Garrity, G. M., Grimont, P. A. D., Kämpfer, P., Maiden, M. C. J., Nesme, X., Rosselló-Mora, R., Swings, J. & other authors(2002). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52, 1043–1047.[CrossRef] [Google Scholar]
  17. Stanier, R. Y., Palleroni, N. J. & Doudoroff, M.(1966). The aerobic pseudomonads: a taxonomic study. J Gen Microbiol 43, 159–271.[CrossRef] [Google Scholar]
  18. Tamaoka, J. & Komagata, K.(1984). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef] [Google Scholar]
  19. Thompson, J. D., Higgins, D. G. & Gibson, T. J.(1994).clustalw: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef] [Google Scholar]
  20. Vanparys, B., Heylen, K., Lebbe, L. & De Vos, P.(2005).Devosia limi sp. nov., isolated from a nitrifying inoculum. Int J Syst Evol Microbiol 55, 1997–2000.[CrossRef] [Google Scholar]
  21. Yoo, S.-H., Weon, H. Y., Kim, B. Y., Hong, S.-B., Kwon, S.-W., Cho, Y.-H., Go, S.-J. & Stackebrandt, E.(2006).Devosia soli sp. nov., isolated from greenhouse soil in Korea. Int J Syst Evol Microbiol 56, 2689–2692.[CrossRef] [Google Scholar]
  22. Yoon, J.-H., Kang, S.-J., Park, S. & Oh, T.-K.(2007).Devosia insulae sp. nov., isolated from soil, and emended description of the genus Devosia. Int J Syst Evol Microbiol 57, 1310–1314.[CrossRef] [Google Scholar]
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Transmission electron micrograph showing the general morphology of negatively stained cells of strain BD-c194 after growth for 3 days at 30 °C on R2A agar. Bar, 1 µm.

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Cellular fatty acid compositions of strain BD-c194 and type strains of related species cultivated on TSA. [PDF](22 KB)

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