A Gram-negative, strictly aerobic, non-spore-forming bacterium, designated strain BD-c194, was isolated from diesel-contaminated soil in Geoje, Korea. The cells were short, motile rods with single polar flagella. Growth of strain BD-c194 was observed between 15 and 45 °C (optimally at 30–35 °C) and between pH 6.0 and 9.5 (optimally at pH 7.5–9.0). The predominant fatty acids were 11-methyl C , C, C , C and an unknown fatty acid (equivalent chain-length 18.814); a large amount of phosphatidylglycerol and a small amount of diphosphatidylglycerol were present as polar lipids. The G+C content of the genomic DNA was 60.8 mol% and the major isoprenoid quinone was Q-10. A comparative 16S rRNA gene sequence analysis showed that strain BD-c194 formed a well-defined phyletic lineage within the genus (with 100 % bootstrap support). The levels of 16S rRNA gene sequence similarity with respect to the type strains of other species ranged from 95.0 to 96.1 %. On the basis of chemotaxonomic data and molecular properties, strain BD-c194 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is BD-c194 (=KCTC 22082 =DSM 19414).


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Transmission electron micrograph showing the general morphology of negatively stained cells of strain BD-c194 after growth for 3 days at 30 °C on R2A agar. Bar, 1 µm.


Cellular fatty acid compositions of strain BD-c194 and type strains of related species cultivated on TSA. [PDF](22 KB)


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