1887

Abstract

The taxonomic positions of 22 spore-forming, extensively branched actinobacteria isolated from soils were examined using a polyphasic approach. Analysis of the 16S rRNA gene sequences indicated that all of the isolates fell into three distinctive phylogenetic clusters within the genus of the family , and also that was the species closest to the three phyloclusters, with 16S rRNA gene sequence similarities ranging from 98.0 to 99.2 %. However, the low DNA–DNA relatedness values between representatives of the three clusters and clearly differentiated them from one another. Representative isolates were also found to have chemotaxonomic features typical of the genus and were distinguishable from all established species of on the basis of a combination of phenotypic properties. It is evident from this study that each of the three phyloclusters should be equated with three novel species, for which the following names are proposed: sp. nov. (type strain AM11 =NBRC 103185 =KCTC 19278), sp. nov. (type strain AM16 =NBRC 103186 =KCTC 19279) and sp. nov. (type strain SB-B34 =NBRC 103184 =KCTC 19280).

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2008-07-01
2024-03-29
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References

  1. Cho, J. C. & Giovannoni, S. J.(2004).Oceanicola granulosus gen. nov., sp. nov. and Oceanicola batsensis sp. nov., poly-β-hydroxybutyrate-producing marine bacteria in the order ‘Rhodobacterales’. Int J Syst Evol Microbiol 54, 1129–1136.[CrossRef] [Google Scholar]
  2. Cho, S. H., Han, J. H., Seong, C. N. & Kim, S. B.(2006). Phylogenetic diversity of acidophilic sporoactinobacteria isolated from various soils. J Microbiol 44, 600–606. [Google Scholar]
  3. El-Waziry, A. M., Tomita, Y., Ling, J. R. & Onodera, R.(1996). Measurement of total and separate stereoisomers of diaminopimelic acid in rumen bacteria by high-performance liquid chromatography. J Chromatogr B Biomed Appl 677, 53–59.[CrossRef] [Google Scholar]
  4. Goodfellow, M. & Dawson, D.(1978). Qualitative and quantitative studies of bacteria colonizing Picea sitchensis litter. Soil Biol Biochem 10, 303–307.[CrossRef] [Google Scholar]
  5. Hagedorn, C.(1976). Influences of soil acidity on Streptomyces populations inhabiting forest soils. Appl Environ Microbiol 32, 368–375. [Google Scholar]
  6. Huang, Y., Cui, Q., Wang, L., Rodriguez, C., Quintana, E., Goodfellow, M. & Liu, Z.(2004).Streptacidiphilus jiangxiensis sp. nov., a novel actinomycete isolated from acidic rhizosphere soil in China. Antonie van Leeuwenhoek 86, 159–165.[CrossRef] [Google Scholar]
  7. Kämpfer, P. & Kroppenstedt, R. M.(1996). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef] [Google Scholar]
  8. Khan, M. R. & Williams, S. T.(1975). Studies on the ecology of actinomycetes in soil. VIII. Distribution and characteristics of acidophilic actinomycetes. Soil Biol Biochem 7, 345–348.[CrossRef] [Google Scholar]
  9. Kim, S. B., Lonsdale, J., Seong, C. N. & Goodfellow, M.(2003).Streptacidiphilus gen. nov., acidophilic actinomycetes with wall chemotype I and emendation of the family Streptomycetaceae (Waksman and Henrici 1943AL) emend. Rainey (1977). Antonie van Leeuwenhoek 83, 107–116.[CrossRef] [Google Scholar]
  10. Kim, S. B., Seong, C. N., Jeon, S. J., Bae, K. S. & Goodfellow, M.(2004). Taxonomic study of neutrotolerant acidophilic actinomycetes isolated from soil and description of Streptomyces yeochonensis sp. nov. Int J Syst Evol Microbiol 54, 211–214.[CrossRef] [Google Scholar]
  11. Komagata, K. & Suzuki, K.(1987). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207. [Google Scholar]
  12. Lonsdale, J. T.(1985).Aspects of the biology of acidophilic actinomycetes. PhD thesis, University of Newcastle, Newcastle, UK.
  13. O'Donnell, A. G., Falconer, C., Goodfellow, M., Ward, A. C. & Williams, E.(1993). Biosystematics and diversity amongst novel carboxydotrophic actinomycetes. Antonie van Leeuwenhoek 64, 325–340. [Google Scholar]
  14. Park, M. S., Jung, S. R., Lee, M. S., Kim, K. O., Do, J. O., Lee, K. H., Kim, S. B. & Bae, K. S.(2005). Isolation and characterization of bacteria associated with two sand dune plant species, Calystegia soldanella and Elymus mollis. J Microbiol 43, 219–227. [Google Scholar]
  15. Park, M. S., Jung, S. R., Lee, K. H., Lee, M. S., Do, J. O., Kim, S. B. & Bae, K. S.(2006).Chryseobacterium soldanellicola sp. nov. and Chryseobacterium taeanense sp. nov., isolated from roots of sand-dune plants. Int J Syst Evol Microbiol 56, 433–438.[CrossRef] [Google Scholar]
  16. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  17. Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  18. Seong, C. N.(1992).Numerical taxonomy of acidophilic and neutrotolerant actinomycetes isolated from acid soil in Korea. PhD thesis, Seoul National University, Seoul, Republic of Korea.
  19. Seong, C. N., Goodfellow, M., Ward, A. C. & Hah, Y. C.(1993). Numerical classification of acidophilic actinomycetes isolated from acid soil in Korea. J Microbiol 31, 355–363. [Google Scholar]
  20. Shirling, E. B. & Gottlieb, D.(1966). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef] [Google Scholar]
  21. Wang, L., Huang, Y., Liu, Z., Goodfellow, M. & Rodriguez, C.(2006).Streptacidiphilus oryzae sp. nov., an actinomycete isolated from rice-field soil in Thailand. Int J Syst Evol Microbiol 56, 1257–1261.[CrossRef] [Google Scholar]
  22. Williams, S. T. & Flowers, T. H.(1978). The influence of pH on starch hydrolysis by neutrophilic and acidophilic actinomycetes. Microbios 20, 99–106. [Google Scholar]
  23. Williams, S. T. & Khan, M. R.(1974). Antibiotics – a soil microbiologist's viewpoint. Postepy Hig Med Dosw 28, 395–408. [Google Scholar]
  24. Williams, S. T. & Robinson, C. S.(1981). The role of streptomycetes in decomposition of chitin in acidic soils. J Gen Microbiol 127, 55–63. [Google Scholar]
  25. Williams, S. T., Davies, F. L., Mayfield, C. I. & Khan, M. R.(1971). Studies on the ecology of actinomycetes in soil. II. The pH requirements of streptomycetes from two acid soils. Soil Biol Biochem 3, 187–195.[CrossRef] [Google Scholar]
  26. Williams, S. T., Goodfellow, M., Alderson, G., Wellington, E. M. H., Sneath, P. H. A. & Sackin, M. J.(1983). Numerical classification of Streptomyces and related genera. J Gen Microbiol 129, 1743–1813. [Google Scholar]
  27. Xu, C., Wang, L., Cui, Q., Huang, Y., Liu, Z., Zheng, G. & Goodfellow, M.(2006). Neutrotolerant acidophilic Streptomyces species isolated from acidic soils in China: Streptomyces guanduensis sp. nov., Streptomyces paucisporeus sp. nov., Streptomyces rubidus sp. nov. and Streptomyces yanglinensis sp. nov. Int J Syst Evol Microbiol 56, 1109–1115.[CrossRef] [Google Scholar]
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Supplements

vol. , part 7, pp. 1566 - 1570

Whole-cell fatty acid profiles of strains AM-11 , AM-16 and SB-B34 and type strains of species with validly published names

Extended neighbour-joining phylogenetic tree, based on almost-complete 16S rRNA gene sequences, showing the relationships between all 22 novel isolates and representatives of the genus .

[PDF file of Supplementary Table S1 and Fig. S1](63 KB)



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