The taxonomic positions of 22 spore-forming, extensively branched actinobacteria isolated from soils were examined using a polyphasic approach. Analysis of the 16S rRNA gene sequences indicated that all of the isolates fell into three distinctive phylogenetic clusters within the genus of the family , and also that was the species closest to the three phyloclusters, with 16S rRNA gene sequence similarities ranging from 98.0 to 99.2 %. However, the low DNA–DNA relatedness values between representatives of the three clusters and clearly differentiated them from one another. Representative isolates were also found to have chemotaxonomic features typical of the genus and were distinguishable from all established species of on the basis of a combination of phenotypic properties. It is evident from this study that each of the three phyloclusters should be equated with three novel species, for which the following names are proposed: sp. nov. (type strain AM11 =NBRC 103185 =KCTC 19278), sp. nov. (type strain AM16 =NBRC 103186 =KCTC 19279) and sp. nov. (type strain SB-B34 =NBRC 103184 =KCTC 19280).


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vol. , part 7, pp. 1566 - 1570

Whole-cell fatty acid profiles of strains AM-11 , AM-16 and SB-B34 and type strains of species with validly published names

Extended neighbour-joining phylogenetic tree, based on almost-complete 16S rRNA gene sequences, showing the relationships between all 22 novel isolates and representatives of the genus .

[PDF file of Supplementary Table S1 and Fig. S1](63 KB)

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