A mycelium-forming actinomycete, designated strain D8-87T, was isolated from a soil collected from a cave in Jeju, Republic of Korea. Comparative 16S rRNA gene sequence analysis showed that the organism was closely related to the type and only species of the genus Jiangella. The level of 16S rRNA gene sequence similarity between strain D8-87T and the type strain of Jiangella gansuensis was 99.2 %. The novel organism produced extensively branched aerial and substrate mycelia, which fragmented into short or elongated rod-shaped cells. Whole-cell hydrolysates of the isolate contained ll-diaminopimelic acid, glucose, mannose and arabinose. The predominant menaquinone was MK-9(H4). The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, phosphatidylinositol mannoside and an unknown phospholipid. The predominant fatty acids were anteiso-C15 : 0, iso-C16 : 0 and iso-C14 : 0. The DNA G+C content of strain D8-87T was 71.5 mol%. Levels of DNA–DNA relatedness between strain D8-87T and J. gansuensis KCTC 19044T were 23.4 and 34.6 %. On the basis of the phenotypic and DNA–DNA hybridization data presented, strain D8-87T is considered to represent a novel species of the genus Jiangella, for which the name Jiangella alkaliphila is proposed. The type strain is D8-87T (=JBRI 2008T=KCTC 19222T=DSM 45079T).
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International Journal of Systematic and Evolutionary
Microbiology vol.
58 , part 5, pp. 1176 - 1179
Supplementary Fig. S1. Scanning electron micrograph of
cells of strain D8-87
Tgrown on ISP 4 medium at 28 °C for 2
weeks.
Supplementary Fig. S2. A neighbour-joining
phylogenetic tree showing the phylogenetic relationships of
strain D8-87
Twithin the radiation of the order
Actinomycetales .