1887

Abstract

A Gram-negative, mesophilic bacterial strain, designated 1-1B, which degrades polycyclic aromatic hydrocarbons, was isolated from petroleum-contaminated seawater during a bioremediation experiment. A 16S rRNA gene sequence analysis indicated that the isolate was affiliated with the genus in the ; the sequence was found to be most similar to those of WP0211 (99.8 %), M-5 (98.2 %) and DSM 14000 (98.1 %). However, the levels of DNA–DNA relatedness between strain 1-1B and these type strains were 50.7±17.2, 35.7±17.8 and 32.0±21.1 %, respectively. In addition, strain 1-1B was found to be distinct from the other described species of the genus in terms of some taxonomically important traits, including DNA G+C content, optimum growth temperature, salinity tolerance, utilization of carbon sources and fatty acid composition. Furthermore, strain 1-1B and were also different with regard to motility and denitrification capacities. On the basis of physiological and DNA–DNA hybridization data, strain 1-1B represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is 1-1B (=JCM 14578 =DSM 18888).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65476-0
2008-03-01
2019-12-13
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/3/711.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65476-0&mimeType=html&fmt=ahah

References

  1. Beveridge, T. J., Popkin, T. J. & Cole, R. M. ( 1994; ). Electron microscopy. In Methods for General and Molecular Bacteriology, pp. 42–71. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  2. Dutta, T. K. & Harayama, S. ( 2000; ). Fate of crude oil by the combination of photooxidation and biodegradation. Environ Sci Technol 34, 1500–1505.[CrossRef]
    [Google Scholar]
  3. Dyksterhouse, S. E., Gray, J. P., Herwig, R. P., Lara, J. C. & Staley, J. T. ( 1995; ). Cycloclasticus pugetii gen. nov., sp. nov., an aromatic hydrocarbon-degrading bacterium from marine sediments. Int J Syst Bacteriol 45, 116–123.[CrossRef]
    [Google Scholar]
  4. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  5. Kasai, Y., Kishira, H. & Harayama, S. ( 2002; ). Bacteria belonging to the genus Cycloclasticus play a primary role in the degradation of aromatic hydrocarbons released in a marine environment. Appl Environ Microbiol 68, 5625–5633.[CrossRef]
    [Google Scholar]
  6. Katayama-Fujimura, Y., Komatsu, Y., Kuraishi, H. & Kaneko, T. ( 1984; ). Estimation of DNA base composition by high performance liquid chromatography of its nuclease P1 hydrolysate. Agric Biol Chem 48, 3169–3172.[CrossRef]
    [Google Scholar]
  7. Kodama, Y. & Watanabe, K. ( 2003; ). Isolation and characterization of a sulfur-oxidizing chemolithotroph growing on crude oil under anaerobic conditions. Appl Environ Microbiol 69, 107–112.[CrossRef]
    [Google Scholar]
  8. Kodama, Y., Ha, L. T. & Watanabe, K. ( 2007; ). Sulfurospirillum cavolei sp. nov., a facultatively anaerobic sulfur-reducing bacterium isolated from an underground crude oil storage cavity. Int J Syst Evol Microbiol 57, 827–831.[CrossRef]
    [Google Scholar]
  9. Liu, C., Wu, Y., Li, L., Ma, Y. & Shao, Z. ( 2007; ). Thalassospira xiamenensis sp. nov. and Thalassospira profundimaris sp. nov. Int J Syst Evol Microbiol 57, 316–320.[CrossRef]
    [Google Scholar]
  10. López-López, A., Pujalte, M. J., Benlloch, S., Mata-Roig, M., Rosselló-Mora, R., Garay, E. & Rodríguez-Valera, F. ( 2002; ). Thalassospira lucentensis gen. nov., sp. nov., a new marine member of the α-Proteobacteria. Int J Syst Evol Microbiol 52, 1277–1283.[CrossRef]
    [Google Scholar]
  11. Murray, M. G. & Thompson, W. F. ( 1980; ). Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8, 4321–4325.[CrossRef]
    [Google Scholar]
  12. Orphan, V. J., Hinrichs, K.-U., Ussler, W., III, Paull, C. K., Taylor, L. T., Sylva, S. P., Hayes, J. M. & Delong, E. F. ( 2001; ). Comparative analysis of methane-oxidizing archaea and sulfate-reducing bacteria in anoxic marine sediments. Appl Environ Microbiol 67, 1922–1934.[CrossRef]
    [Google Scholar]
  13. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–655. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  14. Sohn, J. H., Kwon, K. K., Kang, J.-H., Jung, H.-B. & Kim, S.-J. ( 2004; ). Novosphingobium pentaromativorans sp. nov., a high-molecular-mass polycyclic aromatic hydrocarbon-degrading bacterium isolated from estuarine sediment. Int J Syst Evol Microbiol 54, 1483–1487.[CrossRef]
    [Google Scholar]
  15. Stackebrandt, E. & Ebers, J. ( 2006; ). Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33, 152–155.
    [Google Scholar]
  16. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  17. Watanabe, K., Watanabe, K., Kodama, Y., Syutsubo, K. & Harayama, S. ( 2000; ). Molecular characterization of bacterial populations in petroleum-contaminated groundwater discharged from underground crude oil storage cavities. Appl Environ Microbiol 66, 4803–4809.[CrossRef]
    [Google Scholar]
  18. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65476-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65476-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error